B5EKH7 (SYP_ACIF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Lferr_1791

Organism

Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)) [Complete proteome] [HAMAP]

Taxonomic identifier

380394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	Proline--tRNA ligase HAMAP MF_01569		PRO_1000199339

Sequences

	Sequence		Length	Mass (Da)
	B5EKH7 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 50FFD3E474B93202	FASTA	570	63,598
	10 20 30 40 50 60 MRASQIFIPT LKETPAEAEL VSHQLLLRGG FIRRLASGLY TWMPLGLRVL RKVERVVREE 70 80 90 100 110 120 MDRSGAQELL MPVVQPAELW QESGRWEQYG PELLRLRDRH DREFCLGPTH EEVISDLARR 130 140 150 160 170 180 EIHSYRQLPV NFYQIQTKFR DEIRPRFGLM RGREFIMKDA YSFHMDHSSL EITYQAMYEA 190 200 210 220 230 240 YQRVFTRLGL SFRAVEADNG AIGGKRSHEF HVLADSGEDA IVSCHHCDYA ANMEKAASRP 250 260 270 280 290 300 DLAETEIPLA AERVRTPGIR TVAEQAEHLG IPTAKIVKTV LVVADGKTVM LLLRGDDELN 310 320 330 340 350 360 LVKAGHALNA QDVQMARPEE AISATGAPLG FIGPKEPLLS IPILADHRAL GVANFSTGAN 370 380 390 400 410 420 AADLHWINLN WDRDLPRPAA ADLRNVRAGD ACPHCAEGTL SIRRGIEVGH VFQLGERYSE 430 440 450 460 470 480 SMGITVLDET GRDATVTMGC YGIGVSRVVA AAVEQHFDDR GIIWPVALAP FEVGIVAINA 490 500 510 520 530 540 RKSPDVAAAA QALHDRLEAE GYSVLLDDRD ERPGVQFATM DLVGLPHRIV VSDTVLAQGV 550 560 570 WEYRARRTTE NVLLDETQLM ERLRKEHARG

References

[1]

"Complete sequence of Acidithiobacillus ferrooxidans ATCC 53993." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Borole A.P. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 53993.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001132 Genomic DNA. Translation: ACH84012.1.
RefSeq	YP_002220219.1. NC_011206.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B5EKH7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6877774.
GenomeReviews	Gene locus Lferr_1791 in contig CP001132_GR.
KEGG	afe:Lferr_1791.
PATRIC	20661190. VBIAciFer6930_1791.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG403504.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.90.960.10. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ACIF5

Accession

Primary (citable) accession number: B5EKH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program