Reviewed, UniProtKB/Swiss-Prot B5EJL3 (SYD_ACIF5)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	Lferr_0057

Organism

Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)) [Complete proteome] [HAMAP]

Taxonomic identifier

380394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_1000090951

Sequences

	Sequence		Length	Mass (Da)
	B5EJL3-1 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 5A98C8F4FFD51C6B	FASTA	596	66,372
	10 20 30 40 50 60 MRTHYCNGIH EGLIGQTVTL CGWAQRRRDH GGVIFIDLRD REGLVQIVAD PDQVDAFAAA 70 80 90 100 110 120 NECRSEFVLQ VEGVLRARPA GTENPHMPSG KVELAAARIR ILNRSEPVPF PLDEEDIAEN 130 140 150 160 170 180 LRLKYRYLDL RRPEMLHRLR LRHQVTRFVR GYLDNAGFID VETPVLTRST PEGARDYLVP 190 200 210 220 230 240 SRTQPGHFFA LPQSPQLFKQ LLMVAGLDRY YQITKCFRDE DLRADRQPEF TQIDIEASFV 250 260 270 280 290 300 DEEAVMGTAE PMIRGLFAEV LGVQLPDPFP RMTYRDAMHR FGVDRPDLRN PLELTELTDL 310 320 330 340 350 360 MRAVDFKVFR EAAERPHGRV ACLRVPGGAQ LSRAQIDAYT QFTAIYGARG LAWIKVNALD 370 380 390 400 410 420 QGNEGLQSPI VKFLPEMVLS EILRRSGAAA GDILFFGADT AKIVNEALGN LRNRVAADLG 430 440 450 460 470 480 LLEGEWCPVW ITDFPMFDYD DKEDRWTSTH HPFTAPQTEH LETLGQDPGN ALARAYDLVL 490 500 510 520 530 540 NGNEIGGGSI RIHQEAVQEK VFAALGIGAE EARDKFGFLL DALHYGAPPH GGLAFGLDRL 550 560 570 580 590 VMLLCGAETI RDVIAFPKTQ KAGCLLTEAP GTVADKQLQE LGIRLRPGVG GGVPNP

References

[1]

"Complete sequence of Acidithiobacillus ferrooxidans ATCC 53993." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Borole A.P. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001132 Genomic DNA. Translation: ACH82319.1.
RefSeq	YP_002218526.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6876006.
GenomeReviews	Gene locus Lferr_0057 in contig CP001132_GR.
KEGG	afe:Lferr_0057.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VDRRRDH.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_ACIF5

Accession

Primary (citable) accession number: B5EJL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 14, 2008
Last modified:	November 3, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)