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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi483 – 4831MagnesiumUniRule annotation
Metal bindingi489 – 4891MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciGBEM404380:GHFR-1344-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Gbem_1308
OrganismiGeobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622)
Taxonomic identifieri404380 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
ProteomesiUP000008825 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 696696Polyribonucleotide nucleotidyltransferasePRO_0000381898Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi404380.Gbem_1308.

Structurei

3D structure databases

ProteinModelPortaliB5EI63.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini550 – 60960KHUniRule annotationAdd
BLAST
Domaini619 – 68769S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5EI63-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHTVQAECC GKTITIETGK IAKQASGAVM IKSGDTMVLV TAVAMKSAKE
60 70 80 90 100
GQGFFPLTVN YQEKAYAGGR IPGSFFKREG RPSDNETLTC RLIDRPIRPL
110 120 130 140 150
FPENFLNDTQ IMATVVSADK DNDPGILSMI GASAALMVSD VPFAGPIAGV
160 170 180 190 200
KVGRVDGQFI ANPTAEQEEK SDLEIVIAAS QDAILMVEGS ACEVSEDDLL
210 220 230 240 250
EAIFFGHKAV QPVLAAQLEL AKKVGTSKRE IPAPVVNEAL KARVSALAKE
260 270 280 290 300
GMKQAVRIKT KVERHLAIDA IADETVAALS AEFEGSEKEI KGFIEDLEYD
310 320 330 340 350
LVREHIIKDG QRIDGRDTKT IRAISTEVSL LPRAHGSALF TRGETQSIVA
360 370 380 390 400
ATLGTSVDEQ RIDSLYGDSR KKFMLHYNFP PYSVGETSFR LAPGRREIGH
410 420 430 440 450
GMLAERALQQ VLPKHDDFPY TIRIVSDITE SNGSSSMATV CGGSLSMMDA
460 470 480 490 500
GIPIKAPVAG IAMGLIKEGD DFAILSDILG DEDHLGDMDF KVAGTAEGVT
510 520 530 540 550
ALQMDIKIGG VTREIMSAAL AQAKAGRIHI LGEMAKTIGA SRGDLSAFAP
560 570 580 590 600
RITTIWVKVD KIRDVIGSGG KNIRSVTEAT GVSIDIDDTG KINIASTNKE
610 620 630 640 650
ACDLAIKMIR NLTAEAEEGK LYMGTVKKIM EFGAFVEIFP GTDGLVHVSE
660 670 680 690
LDTERVKNVS DILKEGDKVL VKCIGIDKQG KIKLSRKEAL GLTFTE
Length:696
Mass (Da):74,701
Last modified:October 14, 2008 - v1
Checksum:i14A55B42966E28D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001124 Genomic DNA. Translation: ACH38327.1.
RefSeqiWP_012529738.1. NC_011146.1.

Genome annotation databases

EnsemblBacteriaiACH38327; ACH38327; Gbem_1308.
KEGGigbm:Gbem_1308.
PATRICi21986342. VBIGeoBem56306_1299.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001124 Genomic DNA. Translation: ACH38327.1.
RefSeqiWP_012529738.1. NC_011146.1.

3D structure databases

ProteinModelPortaliB5EI63.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi404380.Gbem_1308.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACH38327; ACH38327; Gbem_1308.
KEGGigbm:Gbem_1308.
PATRICi21986342. VBIGeoBem56306_1299.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciGBEM404380:GHFR-1344-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bem / ATCC BAA-1014 / DSM 16622.

Entry informationi

Entry nameiPNP_GEOBB
AccessioniPrimary (citable) accession number: B5EI63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: October 14, 2008
Last modified: July 22, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.