B5EHW3 (B5EHW3_GEOBB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase HAMAP MF_00435
EC=1.1.1.86 HAMAP MF_00435

Alternative name(s):
Acetohydroxy-acid isomeroreductase HAMAP MF_00435
Alpha-keto-beta-hydroxylacil reductoisomerase HAMAP MF_00435

Gene names

Name:	ilvC HAMAP MF_00435
Ordered Locus Names:	Gbem_2758

Organism

Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) [Complete proteome] [HAMAP]

Taxonomic identifier

404380 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435 SAAS SAAS014359 (R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 SAAS SAAS014359
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 SAAS SAAS014359 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435 SAAS SAAS014359
Sequence similarities	Belongs to the ketol-acid reductoisomerase family. HAMAP MF_00435

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00435 SAAS SAAS014359
Ligand	NADP HAMAP MF_00435 SAAS SAAS014359
Molecular function	Isomerase EMBL ACH39762.1 Oxidoreductase HAMAP MF_00435 SAAS SAAS014359
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	133	1	By similarity HAMAP MF_00435

Sequences

	Sequence		Length	Mass (Da)
	B5EHW3 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 931AF811D781D5DE	FASTA	490	53,871
	10 20 30 40 50 60 MGVNYFNTLP LRRQLQELGT CRFMDASEFA NGCEYVKGKK IVIVGCGAQG LNQGLNMRDS 70 80 90 100 110 120 GLDVSYTLRK EAIEQKRQSY LNASENGFKV GSYEELLPGA DIVMNLAPDK QHSNVVDTVI 130 140 150 160 170 180 PFMKQGAVFS YAHGFNIVEE GTQIRKDLTV VMVAPKCPGS EVRAEYKRGF GVPTLIAVHG 190 200 210 220 230 240 ENDPNGDGLE IAKALASAQG GDRAGVLESS FVAEVKSDLM GEQTILCGML QAGALLCFDK 250 260 270 280 290 300 MTQNGIAAPY AVKLIQYGWE TITEALKHGG ITNMMDRLSN PAKLIAFKLA DELKDIMRPL 310 320 330 340 350 360 FQKHMDDIMS GEFSRTMMED WANDDVKLLT WREQTGQTAF EKTEAAGEIS EQEYFDKAIL 370 380 390 400 410 420 MVAMVKAGVE LAFETMVQTG IEPESAYYES LHETPLIANT IARKKLYEMN RVISDTAEYG 430 440 450 460 470 480 CYLFSHACVP LLKDFMSKVG TDVIGKGLAA KDNSVDNQLL VAVNAEIRSH LVEEVGAELR 490 AAMKGMQKIV

References

[1]

"Complete sequence of Geobacter bemidjiensis BEM." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Lovley D., Richardson P. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bem / ATCC BAA-1014 / DSM 16622.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001124 Genomic DNA. Translation: ACH39762.1.
RefSeq	YP_002139558.1. NC_011146.1.
3D structure databases
ProteinModelPortal	B5EHW3.
ModBase	Search...
Protein-protein interaction databases
STRING	B5EHW3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6782756.
GenomeReviews	Gene locus Gbem_2758 in contig CP001124_GR.
KEGG	gbm:Gbem_2758.
PATRIC	21989200. VBIGeoBem56306_2715.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG297205.
OMA	EKHMDDI.
ProtClustDB	PRK05225.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. IPR014359. KetolA_reductoisomerase_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit. PTHR21371:SF2. PTHR21371:SF2. 1 hit.
Pfam	PF01450. IlvC. 2 hits. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000116. IlvC_gammaproteo. 1 hit.
SUPFAM	SSF48179. 6DGDH_C_like. 2 hits.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B5EHW3_GEOBB

Accession

Primary (citable) accession number: B5EHW3

Entry history

Integrated into UniProtKB/TrEMBL:	October 14, 2008
Last sequence update:	October 14, 2008
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)