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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciGBEM404380:GHFR-2764-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:Gbem_2710
OrganismiGeobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622)
Taxonomic identifieri404380 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
Proteomesi
  • UP000008825 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_1000213382Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi404380.Gbem_2710.

Structurei

3D structure databases

ProteinModelPortaliB5EHR5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091H00B3.

Family and domain databases

Gene3Di3.50.50.60. 4 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5EHR5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQEITVIGG GLAGCEAAWQ AAKRGVKVRL FEMKPNCYSE AHHLPGLSEL
60 70 80 90 100
VCSNSLRGDS LENAVGLLKE ELRRLQSLFM EGAEATKVPA GGALAVDRDL
110 120 130 140 150
FSQYITSRIE SHPLIEVVRE EVTRIPEEGI VVLASGPLTS GLLAEEIGRL
160 170 180 190 200
AGSYLYFYDA IAPIVAADSI DYDKAFRASR YGKGDGDDYL NCPMDEEQYQ
210 220 230 240 250
AFVREIVAAE KVEPKSFEKV VHFEGCMPIE EMASRGPETL RFGPMKPVGL
260 270 280 290 300
VDPRVGVEPH AVIQLRQENR EATMYNLVGF QTKLTWPEQK RIFRMIPGLE
310 320 330 340 350
NAQFLRLGSM HRNTFINAPE LLMATCQLKS DQRIFFAGQI TGVEGYVESA
360 370 380 390 400
SSGFVAGVNA ARLAQGEGLV VPPAETAIGA LARHITNTEA GHFQPMNVNY
410 420 430
GLFPSLPGRV KKKEKRGLLA QRGLETLEKW LPELS
Length:435
Mass (Da):47,840
Last modified:October 14, 2008 - v1
Checksum:iFE9BD7EF1C64BB12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001124 Genomic DNA. Translation: ACH39714.1.
RefSeqiWP_012531140.1. NC_011146.1.

Genome annotation databases

EnsemblBacteriaiACH39714; ACH39714; Gbem_2710.
KEGGigbm:Gbem_2710.
PATRICi21989102. VBIGeoBem56306_2666.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001124 Genomic DNA. Translation: ACH39714.1.
RefSeqiWP_012531140.1. NC_011146.1.

3D structure databases

ProteinModelPortaliB5EHR5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi404380.Gbem_2710.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACH39714; ACH39714; Gbem_2710.
KEGGigbm:Gbem_2710.
PATRICi21989102. VBIGeoBem56306_2666.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091H00B3.

Enzyme and pathway databases

BioCyciGBEM404380:GHFR-2764-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 4 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_GEOBB
AccessioniPrimary (citable) accession number: B5EHR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: October 14, 2008
Last modified: September 7, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.