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B5EFY8 (NADK_GEOBB) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:Gbem_2445
OrganismGeobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) [Complete proteome] [HAMAP]
Taxonomic identifier404380 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 288288NAD kinase HAMAP-Rule MF_00361
PRO_1000120862

Regions

Nucleotide binding70 – 712NAD By similarity
Nucleotide binding144 – 1452NAD By similarity
Nucleotide binding185 – 1906NAD By similarity

Sites

Active site701Proton acceptor By similarity
Binding site1551NAD By similarity
Binding site1721NAD By similarity
Binding site1741NAD By similarity
Binding site2451NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
B5EFY8 [UniParc].

Last modified October 14, 2008. Version 1.
Checksum: AD055C58E09B3301

FASTA28831,349
        10         20         30         40         50         60 
MKKIAIFAKV HDPRALAVAE ELIEWLAARG VTAHVEEHLS KRLRRTTLAE SSESTEIAAD 

        70         80         90        100        110        120 
ADLVVVLGGD GTLIAAARLV GERDVPILAV NLGSLGFLTE ITLNELYPSV ERCLAGDFEV 

       130        140        150        160        170        180 
SERMMLMASV ERSGEVVELH RVLNDVVINK GALARIIDME TSVNGRYLTT FKADGLIVST 

       190        200        210        220        230        240 
PTGSTGYSLS ANGPILHPEL ECISLTPICP HTLTNRPLVM AADAHIAIKL KYAPDESVFL 

       250        260        270        280 
TLDGQVGMKL LSGDVVQITK AAHVTRLIQS RSKDYFEVLR TKLKWGER 

« Hide

References

[1]"Complete sequence of Geobacter bemidjiensis BEM."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Lykidis A., Lovley D., Richardson P.
Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bem / ATCC BAA-1014 / DSM 16622.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001124 Genomic DNA. Translation: ACH39453.1.
RefSeqYP_002139249.1. NC_011146.1.

3D structure databases

ProteinModelPortalB5EFY8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING404380.Gbem_2445.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACH39453; ACH39453; Gbem_2445.
GeneID6782442.
KEGGgbm:Gbem_2445.
PATRIC21988572. VBIGeoBem56306_2403.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227222.
KOK00858.
OMAHPSIPGW.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycGBEM404380:GHFR-2497-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_GEOBB
AccessionPrimary (citable) accession number: B5EFY8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 14, 2008
Last modified: July 9, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families