B5EFG3 (NUOA2_GEOBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit A 2
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit A 2
NDH-1 subunit A 2
NUO1 2

Gene names

Name:	nuoA2
Ordered Locus Names:	Gbem_3926

Organism

Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) [Complete proteome] [HAMAP]

Taxonomic identifier

404380 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01394
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01394.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	NADH-quinone oxidoreductase subunit A 2 HAMAP MF_01394		PRO_0000362690
Regions
	Transmembrane	5 – 25	21	Helical; Potential
	Transmembrane	62 – 82	21	Helical; Potential
	Transmembrane	87 – 107	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	B5EFG3 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: D45B7F74EA2DB5CA	FASTA	118	13,280
	10 20 30 40 50 60 MLGAYLPILV LVVIAVLFGL GSVIFSSLIG QKKPSTVKLA PYECGCEPVG SARERFSIKF 70 80 90 100 110 YLIAMLFILF DIEAVFLYPW AVLFKRLGMF GLIEMGVFIV ILFVGYVYVW KKGALEWE

References

[1]

"Complete sequence of Geobacter bemidjiensis BEM." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Lovley D., Richardson P. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bem / ATCC BAA-1014 / DSM 16622.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001124 Genomic DNA. Translation: ACH40918.1.
RefSeq	YP_002140714.1. NC_011146.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B5EFG3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6780790.
GenomeReviews	Gene locus Gbem_3926 in contig CP001124_GR.
KEGG	gbm:Gbem_3926.
PATRIC	21991470. VBIGeoBem56306_3836.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG605540.
OMA	IGFAYEW.
ProtClustDB	CLSK827761.
Family and domain databases
HAMAP	MF_01394. NDH1_NuoA. [Tree]
InterPro	IPR023043. NAD(P)H_OxRDtase_bac/plastid. IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
KO	K00330.
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA2_GEOBB

Accession

Primary (citable) accession number: B5EFG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program