Reviewed, UniProtKB/Swiss-Prot B5EF56 (CYSD_GEOBB)

Names and origin

Protein names

Recommended name:
    Sulfate adenylyltransferase subunit 2
    EC=2.7.7.4
Alternative name(s):
    Sulfate adenylate transferase
      Short name=SAT
    ATP-sulfurylase small subunit

Gene names

Name:	cysD
Ordered Locus Names:	Gbem_2353

Organism

Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) [Complete proteome] [HAMAP]

Taxonomic identifier

404380 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of cysD, the smaller subunit, and cysN By similarity. HAMAP MF_00064
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate assimilation Inferred from electronic annotation. Source: HAMAP sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_1000092205

Sequences

	Sequence		Length	Mass (Da)
	B5EF56-1 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 35E54D3B78192D28	FASTA	301	34,848
	10 20 30 40 50 60 MTTQLTHLQQ LEAESIHIIR EVVAEFDNPV MLYSIGKDSA VMLHLARKAF YPAPPPFPLL 70 80 90 100 110 120 HVDTTWKFRE MIQFRGRMAK ESGMELLVHV NEDGVKRGVS PFTHGSALYT DVMKTEGLKQ 130 140 150 160 170 180 ALDHYKFDAA FGGARRDEEK SRAKERIFSF RSANHRWDPK NQRPELWSLY NTRIKPGESI 190 200 210 220 230 240 RVFPLSNWTE LDIWQYIHLE QIPIVPLYYA AVRPVVERDG MLIMVDDDRL ELKPGEKVEQ 250 260 270 280 290 300 KSVRFRTLGC YPLTGAVESE ADTLPEIIQE MLLTRTSERQ GRLIDHDQAG SMEKKKQEGY F

References

[1]

"Complete sequence of Geobacter bemidjiensis BEM." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Lovley D., Richardson P. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001124 Genomic DNA. Translation: ACH39365.1.
RefSeq	YP_002139161.1.
3D structure databases
SMR	B5EF56. Positions 1-211, 14-283.
ModBase	Search...
Genome annotation databases
GeneID	6782352.
GenomeReviews	Gene locus Gbem_2353 in contig CP001124_GR.
KEGG	gbm:Gbem_2353.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG758022.
OMA	KTSERQG.
Family and domain databases
HAMAP	MF_00064. Sulf_adenylyltr_sub2. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_GEOBB

Accession

Primary (citable) accession number: B5EF56

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 14, 2008
Last modified:	February 9, 2010
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)