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Protein

Bifunctional protein HldE

Gene

hldE

Organism
Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to selectively form D-glycero-beta-D-manno-heptose-1,7-bisphosphate.UniRule annotation
Catalyzes the ADP transfer from ATP to D-glycero-beta-D-manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno-heptose.UniRule annotation

Catalytic activityi

D-glycero-beta-D-manno-heptose 7-phosphate + ATP = D-glycero-beta-D-manno-heptose 1,7-bisphosphate + ADP.UniRule annotation
D-glycero-beta-D-manno-heptose 1-phosphate + ATP = ADP-D-glycero-beta-D-manno-heptose + diphosphate.UniRule annotation

Pathwayi: ADP-L-glycero-beta-D-manno-heptose biosynthesis

This protein is involved in step 1 and 3 of the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Bifunctional protein HldE (hldE)
  2. no protein annotated in this organism
  3. Bifunctional protein HldE (hldE)
  4. no protein annotated in this organism
This subpathway is part of the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate, the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei275UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi205 – 208ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
Biological processCarbohydrate metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciGBEM404380:G1GCH-874-MONOMER
UniPathwayiUPA00356; UER00437
UPA00356; UER00439

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein HldEUniRule annotation
Including the following 2 domains:
D-beta-D-heptose 7-phosphate kinaseUniRule annotation (EC:2.7.1.167UniRule annotation)
Alternative name(s):
D-beta-D-heptose 7-phosphotransferaseUniRule annotation
D-glycero-beta-D-manno-heptose-7-phosphate kinaseUniRule annotation
D-beta-D-heptose 1-phosphate adenylyltransferaseUniRule annotation (EC:2.7.7.70UniRule annotation)
Alternative name(s):
D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferaseUniRule annotation
Gene namesi
Name:hldEUniRule annotation
Ordered Locus Names:Gbem_0864
OrganismiGeobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622)
Taxonomic identifieri404380 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
Proteomesi
  • UP000008825 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001481251 – 487Bifunctional protein HldEAdd BLAST487

Proteomic databases

PRIDEiB5EEZ1

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi404380.Gbem_0864

Structurei

3D structure databases

ProteinModelPortaliB5EEZ1
SMRiB5EEZ1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 326RibokinaseAdd BLAST326
Regioni356 – 487CytidylyltransferaseAdd BLAST132

Sequence similaritiesi

In the N-terminal section; belongs to the carbohydrate kinase PfkB family.UniRule annotation
In the C-terminal section; belongs to the cytidylyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DW0 Bacteria
COG2870 LUCA
HOGENOMiHOG000237584
KOiK03272
OMAiCDPKGKD
OrthoDBiPOG091H01Y4

Family and domain databases

CDDicd01172 RfaE_like, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_01603 HldE, 1 hit
InterProiView protein in InterPro
IPR023030 Bifunc_HldE
IPR004821 Cyt_trans-like
IPR011611 PfkB_dom
IPR011913 RfaE_dom_I
IPR011914 RfaE_dom_II
IPR029056 Ribokinase-like
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF01467 CTP_transf_like, 1 hit
PF00294 PfkB, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00125 cyt_tran_rel, 1 hit
TIGR02198 rfaE_dom_I, 1 hit
TIGR02199 rfaE_dom_II, 1 hit

Sequencei

Sequence statusi: Complete.

B5EEZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERREVESFF ERATSIRALV VGDLMLDEYL WGRAERISPE APVQVVEVAR
60 70 80 90 100
EDLRLGGAGN VVNNLAALGC QVSVCSVIGS DENGALLRRA LEEKGAGLEG
110 120 130 140 150
LFEEGERRTS KKTRVLAANQ QIVRIDRETK SSIAAASEQG IIDYLAARSG
160 170 180 190 200
DFDVIVVSDY LKGVLTPAVL SAVCRSGREL GIPVVVDPKG NDYGKYRGAT
210 220 230 240 250
ILTPNRKEAE IASGVAITDQ ESLQCAASGL LSCLELDALL ITRSEAGMSL
260 270 280 290 300
FPAAGGAVHI PTVAREVFDV TGAGDTVISV LSLGLACGLT MADAAWIANV
310 320 330 340 350
AAGIAVGKLG TSTVAPQEIV AEVGHGAKDS DSKIKNLDVL AHAIAREHSR
360 370 380 390 400
GKKVVFTNGC FDLLHVGHVK YLQKARGLGD ILVVGLNTDA SVRRLKGEKR
410 420 430 440 450
PLIEETERAH ILAALDCIDY VVLFDEDTPL KVIETLGPDI LVKGGDYSIE
460 470 480
GVVGREVVEA RGGRVELIQF VDGRSTSRII EKILSSY
Length:487
Mass (Da):51,836
Last modified:October 14, 2008 - v1
Checksum:i2D287038CA649396
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001124 Genomic DNA Translation: ACH37887.1
RefSeqiWP_012529298.1, NC_011146.1

Genome annotation databases

EnsemblBacteriaiACH37887; ACH37887; Gbem_0864
KEGGigbm:Gbem_0864

Similar proteinsi

Entry informationi

Entry nameiHLDE_GEOBB
AccessioniPrimary (citable) accession number: B5EEZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 14, 2008
Last modified: May 23, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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