B5EEF0 (SYR_GEOBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	Gbem_2283

Organism

Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) [Complete proteome] [HAMAP]

Taxonomic identifier

404380 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity. HAMAP MF_00123
Subcellular location	Cytoplasm By similarity HAMAP MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Arginine--tRNA ligase HAMAP MF_00123		PRO_1000095367
Regions
	Motif	129 – 139	11	"HIGH" region HAMAP MF_00123

Sequences

	Sequence		Length	Mass (Da)
	B5EEF0 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 3D44CDE9A882E3C5	FASTA	554	61,652
	10 20 30 40 50 60 MKEQLRACIL KGIEGCFADG TLTSGEVPAI NVEKPAHAEH GDFATNVAMQ MAKQQRKAPR 70 80 90 100 110 120 AVAEILVAKL AGASDLIESL EIAGPGFINF FIKDSAWRRT LTEIDRAGDA WGKSGIGRGK 130 140 150 160 170 180 KVQVEFVSAN PTGPLHIGHG RGAATGDAVA SLLSAAGFDV QREYYINDAG NQMNTLGLSG 190 200 210 220 230 240 LLRYKELLGE KIDFPETCYQ GDYMKDIARD AVTKYGDRFL KVSQEDGVAF FSKMGGDLIL 250 260 270 280 290 300 AGIDQDLQDF GIRFDHWFSE QSLFDEGKVK SAIEEMQAKG LIYEQEGALW FRTTDYGDDK 310 320 330 340 350 360 DRVVVRSNGV TTYFASDIAY HRDKFARGFD WVIDVWGADH HGYVPRLKSV VQGLGRDASD 370 380 390 400 410 420 LGIILVQLVS LLRDGVPVAM STRSGEFVTL KEVVDEVGRD AARFFFLMRR SDSQLDFDLE 430 440 450 460 470 480 LAKRQSNDNP VYYVQYAHAR IKSIFDTARE RGVEPRFDSV KLELLQTPED LSLVKKLSVY 490 500 510 520 530 540 PEILEGGAVN FEPHRITYYL QELAGEFHSF YNKSRVITPE EPELTQARLF LLHCVAITLK 550 NALTVLGISA PERM

References

[1]

"Complete sequence of Geobacter bemidjiensis BEM." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Lovley D., Richardson P. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bem / ATCC BAA-1014 / DSM 16622.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001124 Genomic DNA. Translation: ACH39295.1.
RefSeq	YP_002139091.1. NC_011146.1.
3D structure databases
ProteinModelPortal	B5EEF0.
ModBase	Search...
Protein-protein interaction databases
STRING	B5EEF0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6782279.
GenomeReviews	Gene locus Gbem_2283 in contig CP001124_GR.
KEGG	gbm:Gbem_2283.
PATRIC	21988238. VBIGeoBem56306_2239.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG695395.
OMA	FDVYFHE.
ProtClustDB	PRK01611.
Family and domain databases
HAMAP	MF_00123. Arg_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01887.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. ArgS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_GEOBB

Accession

Primary (citable) accession number: B5EEF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program