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B5ECN1 (BIOB_GEOBB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Gbem_2053
OrganismGeobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) [Complete proteome] [HAMAP]
Taxonomic identifier404380 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 328328Biotin synthase HAMAP-Rule MF_01694
PRO_0000381400

Sites

Metal binding661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1421Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2021Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B5ECN1 [UniParc].

Last modified October 14, 2008. Version 1.
Checksum: 87AAD7361985B768

FASTA32835,748
        10         20         30         40         50         60 
MEKMINDIAH RIIAGGSITE AEAIQLTQVQ GTEVYDLFRA ATRVKEHFVG NEVHLCSIIN 

        70         80         90        100        110        120 
AKSGRCAENC AFCAQSAHHK TDAPVYPLVQ EEEMLASARM AETNGSACFG IITSGTTVNG 

       130        140        150        160        170        180 
PELEQILTAL RRIRKETTIL PSCSLGIIDE ETARKLKEAG MDTYHHNLET AASFFPQICT 

       190        200        210        220        230        240 
THDYQDDVNT VRAVKKAGVK VCCGGIFGLG ESAAQRVEMA LTLKDLDVDS VPMNFLNPIE 

       250        260        270        280        290        300 
GTRLEGAANI TAQECLKTIA IYRLILPGKR ITVCGGREKN LRDLQSWIFF AGANGTMIGN 

       310        320 
YLTTLGRNVD TDLTMFSDLG LKTVMCAH 

« Hide

References

[1]"Complete sequence of Geobacter bemidjiensis BEM."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Lykidis A., Lovley D., Richardson P.
Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bem / ATCC BAA-1014 / DSM 16622.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001124 Genomic DNA. Translation: ACH39066.1.
RefSeqYP_002138862.1. NC_011146.1.

3D structure databases

ProteinModelPortalB5ECN1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING404380.Gbem_2053.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACH39066; ACH39066; Gbem_2053.
GeneID6782047.
KEGGgbm:Gbem_2053.
PATRIC21987790. VBIGeoBem56306_2017.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK828421.

Enzyme and pathway databases

BioCycGBEM404380:GHFR-2103-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_GEOBB
AccessionPrimary (citable) accession number: B5ECN1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 14, 2008
Last modified: February 19, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways