B5EBH0 (B5EBH0_GEOBB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Delta-aminolevulinic acid dehydratase RuleBase RU000515
EC=4.2.1.24 RuleBase RU000515

Gene names

Name:	hemB EMBL ACH37439.1
Ordered Locus Names:	Gbem_0410

Organism

Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) [Complete proteome] [HAMAP]

Taxonomic identifier

404380 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O. RuleBase RU000515
Subunit structure	Homooctamer By similarity. RuleBase RU000515 PIRSR PIRSR001415-3
Sequence similarities	Belongs to the ALADH family. RuleBase RU004161

Ontologies

Keywords
Biological process	Porphyrin biosynthesis RuleBase RU000515
Ligand	Magnesium PIRSR PIRSR001415-5 Metal-binding PIRSR PIRSR001415-5 Zinc PIRSR PIRSR001415-3
Molecular function	Lyase RuleBase RU000515
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	195	1	Schiff-base intermediate with substrate By similarity PIRSR PIRSR001415-1
	Active site	248	1	Schiff-base intermediate with substrate By similarity PIRSR PIRSR001415-1
	Metal binding	120	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	122	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	130	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	233	1	Magnesium By similarity PIRSR PIRSR001415-5
	Binding site	205	1	Substrate 1 By similarity PIRSR PIRSR001415-2
	Binding site	217	1	Substrate 1 By similarity PIRSR PIRSR001415-2
	Binding site	274	1	Substrate 2 By similarity PIRSR PIRSR001415-2
	Binding site	313	1	Substrate 2 By similarity PIRSR PIRSR001415-2

Sequences

	Sequence		Length	Mass (Da)
	B5EBH0 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 1C5242C3F49B9024	FASTA	339	37,894
	10 20 30 40 50 60 MFYPLYRARR IRGKEVFRNM VRETSLSAND LIYPMFSAFG KGIKKEISSM PGIYQQSIEN 70 80 90 100 110 120 IVEEAQEVYE LGVPAVILFG IPEQKDAMGT DAYSDTGIIQ ETIRAIKKSV PKLAVITDVC 130 140 150 160 170 180 MCEYTDHGHC GVIKNGDVDN DETLELLAKE ALSHAKAGAD MVAPSDMMDG RVAAIRESLD 190 200 210 220 230 240 NNGFNQIPLM SYAVKYASGY YGPFREAAES TPQFGDRRSY QMDPANRLEA IREARMDVEE 250 260 270 280 290 300 GADILMVKPG LPYLDIVREV RNEFNLPTAV YNVSGEYSMI KSAAKMGWID EDRVIMETMM 310 320 330 SFKRAGADLI LTYHSKEVAK LLRKGYEADI SDRCGCGCR

References

[1]

"Complete sequence of Geobacter bemidjiensis BEM." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Lovley D., Richardson P. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bem / ATCC BAA-1014 / DSM 16622.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001124 Genomic DNA. Translation: ACH37439.1.
RefSeq	YP_002137235.1. NC_011146.1.
3D structure databases
ProteinModelPortal	B5EBH0.
SMR	B5EBH0. Positions 5-323.
ModBase	Search...
Protein-protein interaction databases
STRING	B5EBH0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6783236.
GenomeReviews	Gene locus Gbem_0410 in contig CP001124_GR.
KEGG	gbm:Gbem_0410.
PATRIC	21984483. VBIGeoBem56306_0406.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285270.
OMA	ADLCFCE.
ProtClustDB	PRK09283.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001731. Porphobilinogen_synth. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01698.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
SMART	SM01004. ALAD. 1 hit. [Graphical view]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B5EBH0_GEOBB

Accession

Primary (citable) accession number: B5EBH0

Entry history

Integrated into UniProtKB/TrEMBL:	October 14, 2008
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)