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B5EB79 (GLPK_GEOBB) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glycerol kinase
EC=2.7.1.30
Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK
Gene names
Name:glpK
Ordered Locus Names:Gbem_3376
OrganismGeobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) [Complete proteome] [HAMAP]
Taxonomic identifier404380 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length495 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in the regulation of glycerol uptake and metabolism By similarity. HAMAP MF_00186

Catalytic activity

ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186

Sequence similarities

Belongs to the FGGY kinase family.

Ontologies

Keywords
   Biological processGlycerol metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglycerol-3-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glycerol kinase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 495495Glycerol kinase HAMAP MF_00186
PRO_1000098732

Regions

Nucleotide binding408 – 4125ATP By similarity

Sites

Binding site111Substrate By similarity
Binding site151ATP By similarity
Binding site811Substrate By similarity
Binding site1331Substrate By similarity
Binding site2421Substrate By similarity
Binding site2641ATP By similarity
Binding site3071ATP; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
B5EB79 [UniParc].

Last modified October 14, 2008. Version 1.
Checksum: 5AE139843B130B50

FASTA49553,107
        10         20         30         40         50         60 
MEYLLSIDQG TTSSRATLYA ASGESLATAS RPLAQHYPNP GWVEHDPQEI WEGQLACITE 

        70         80         90        100        110        120 
AVAKAGIAPS QIAGVGITNQ RETTVVWERE TGLPLHRAIV WQDRRTAELT ESLKEQGLEA 

       130        140        150        160        170        180 
MVRERTGLLL DPYFSASKLC WLLDRVDGLR QRAERGEVCF GTVDSWLMFK LSGGKSHLTD 

       190        200        210        220        230        240 
ISNASRTMLF NINTLEWDEE LLRLFRIPRG MLPEVRGSAA GFGHTSGEVA GAEIHIAGVA 

       250        260        270        280        290        300 
GDQQAALFGQ GCFAPGMAKA TFGTGAFVVM NSGARPGVGD GALSTIAWQL PGEAVQYALE 

       310        320        330        340        350        360 
GSIFIAGAAV QWLQEGLGLI GNAREVEVLA ASVPDSGGVY FVPALSGLGT PYWDPYARGV 

       370        380        390        400        410        420 
VAGLTRGSTK AHLARAALEA IAFQTLDAIR AMEQASGIAL KELRVDGGAA ANNLLLQIQA 

       430        440        450        460        470        480 
DLLGVPVLRP RCTESTSLGA AFLAGIGAGV LDTSAIAAQW ALDRRFEPQM ERERREELHR 

       490 
GWQKCVRLSL GWEKN

« Hide

References

[1]"Complete sequence of Geobacter bemidjiensis BEM."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Lykidis A., Lovley D., Richardson P.
Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bem / ATCC BAA-1014 / DSM 16622.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001124 Genomic DNA. Translation: ACH40371.1.
RefSeqYP_002140167.1. NC_011146.1.

3D structure databases

ProteinModelPortalB5EB79.
SMRB5EB79. Positions 3-494.
ModBaseSearch...

Protein-protein interaction databases

STRINGB5EB79.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6780234.
GenomeReviewsGene locus Gbem_3376 in contig CP001124_GR.
KEGGgbm:Gbem_3376.
PATRIC21990382. VBIGeoBem56306_3299.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG511469.
OMACYQTRDL.
ProtClustDBCLSK2544915.

Family and domain databases

HAMAPMF_00186. Glycerol_kin.
[Tree]
InterProIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
KOK00864.
PANTHERPTHR10196. FGGY_kin. 1 hit.
PTHR10196:SF9. Glycerol_kin. 1 hit.
PfamPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01311. Glycerol_kin. 1 hit.
PROSITEPS00933. FGGY_KINASES_1. False negative.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLPK_GEOBB
AccessionPrimary (citable) accession number: B5EB79
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 14, 2008
Last modified: January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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