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B5EAX0

- LIPA_GEOBB

UniProt

B5EAX0 - LIPA_GEOBB

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Protein
Lipoyl synthase
Gene
lipA, Gbem_0400
Organism
Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi35 – 351Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi40 – 401Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi46 – 461Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi61 – 611Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi65 – 651Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi68 – 681Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciGBEM404380:GHFR-402-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Gbem_0400
OrganismiGeobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622)
Taxonomic identifieri404380 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
ProteomesiUP000008825: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Lipoyl synthaseUniRule annotation
PRO_1000099605Add
BLAST

Proteomic databases

PRIDEiB5EAX0.

Interactioni

Protein-protein interaction databases

STRINGi404380.Gbem_0400.

Structurei

3D structure databases

ProteinModelPortaliB5EAX0.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiITRRPEW.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B5EAX0-1 [UniParc]FASTAAdd to Basket

« Hide

MDPIRKPAWL QKKIIPAAHA EMEGLLKELR LNTVCQQARC PNITECFGKR    50
QATFLILGRI CTRLCSFCSV SKETPLPLEP GEAASVAEAV KRLGLSHVVI 100
TSPTRDDLPD GGASVYAETV ARIRSVSPAT KVELLIPDFR GDWAALAAVV 150
ESAPDILGHN LETVPRLYSI RSGADYRRSL DLLAQARRMA PGLNTKSGLM 200
LGLGEEEAEL FAVMEDLLKA GCGYLSLGQY LAPSRMHHPV QRYVEPELFE 250
RYKERALTMG FEHVESAPYV RSSYHAENYL ESKFPPPEGE G 291
Length:291
Mass (Da):32,136
Last modified:October 14, 2008 - v1
Checksum:i3DEAD780E6B54CEC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001124 Genomic DNA. Translation: ACH37429.1.
RefSeqiWP_012528837.1. NC_011146.1.
YP_002137225.1. NC_011146.1.

Genome annotation databases

EnsemblBacteriaiACH37429; ACH37429; Gbem_0400.
GeneIDi6783226.
KEGGigbm:Gbem_0400.
PATRICi21984463. VBIGeoBem56306_0396.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001124 Genomic DNA. Translation: ACH37429.1 .
RefSeqi WP_012528837.1. NC_011146.1.
YP_002137225.1. NC_011146.1.

3D structure databases

ProteinModelPortali B5EAX0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 404380.Gbem_0400.

Proteomic databases

PRIDEi B5EAX0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACH37429 ; ACH37429 ; Gbem_0400 .
GeneIDi 6783226.
KEGGi gbm:Gbem_0400.
PATRICi 21984463. VBIGeoBem56306_0396.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi ITRRPEW.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci GBEM404380:GHFR-402-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bem / ATCC BAA-1014 / DSM 16622.

Entry informationi

Entry nameiLIPA_GEOBB
AccessioniPrimary (citable) accession number: B5EAX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 14, 2008
Last modified: September 3, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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