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B5EAX0

- LIPA_GEOBB

UniProt

B5EAX0 - LIPA_GEOBB

Protein

Lipoyl synthase

Gene

lipA

Organism
Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (14 Oct 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi35 – 351Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi40 – 401Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi46 – 461Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi61 – 611Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi65 – 651Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi68 – 681Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciGBEM404380:GHFR-402-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:Gbem_0400
    OrganismiGeobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622)
    Taxonomic identifieri404380 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
    ProteomesiUP000008825: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 291291Lipoyl synthasePRO_1000099605Add
    BLAST

    Proteomic databases

    PRIDEiB5EAX0.

    Interactioni

    Protein-protein interaction databases

    STRINGi404380.Gbem_0400.

    Structurei

    3D structure databases

    ProteinModelPortaliB5EAX0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiITRRPEW.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B5EAX0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPIRKPAWL QKKIIPAAHA EMEGLLKELR LNTVCQQARC PNITECFGKR    50
    QATFLILGRI CTRLCSFCSV SKETPLPLEP GEAASVAEAV KRLGLSHVVI 100
    TSPTRDDLPD GGASVYAETV ARIRSVSPAT KVELLIPDFR GDWAALAAVV 150
    ESAPDILGHN LETVPRLYSI RSGADYRRSL DLLAQARRMA PGLNTKSGLM 200
    LGLGEEEAEL FAVMEDLLKA GCGYLSLGQY LAPSRMHHPV QRYVEPELFE 250
    RYKERALTMG FEHVESAPYV RSSYHAENYL ESKFPPPEGE G 291
    Length:291
    Mass (Da):32,136
    Last modified:October 14, 2008 - v1
    Checksum:i3DEAD780E6B54CEC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001124 Genomic DNA. Translation: ACH37429.1.
    RefSeqiWP_012528837.1. NC_011146.1.
    YP_002137225.1. NC_011146.1.

    Genome annotation databases

    EnsemblBacteriaiACH37429; ACH37429; Gbem_0400.
    GeneIDi6783226.
    KEGGigbm:Gbem_0400.
    PATRICi21984463. VBIGeoBem56306_0396.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001124 Genomic DNA. Translation: ACH37429.1 .
    RefSeqi WP_012528837.1. NC_011146.1.
    YP_002137225.1. NC_011146.1.

    3D structure databases

    ProteinModelPortali B5EAX0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 404380.Gbem_0400.

    Proteomic databases

    PRIDEi B5EAX0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACH37429 ; ACH37429 ; Gbem_0400 .
    GeneIDi 6783226.
    KEGGi gbm:Gbem_0400.
    PATRICi 21984463. VBIGeoBem56306_0396.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi ITRRPEW.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci GBEM404380:GHFR-402-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bem / ATCC BAA-1014 / DSM 16622.

    Entry informationi

    Entry nameiLIPA_GEOBB
    AccessioniPrimary (citable) accession number: B5EAX0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: October 14, 2008
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3