B5E972 (NUBCD_GEOBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit B/C/D
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit B/C/D
NDH-1 subunit B/C/D

Gene names

Name:	nuoBCD
Synonyms:	nuoB, nuoC, nuoD
Ordered Locus Names:	Gbem_0178

Organism

Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) [Complete proteome] [HAMAP]

Taxonomic identifier

404380 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	794 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01356
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01356
Subunit structure	NDH-1 is composed of about 13 different subunits. Subunits NuoBCD, E, F, and G constitute the peripheral sector of the complex By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_01356.
Sequence similarities	In the N-terminal section; belongs to the complex I 20 kDa subunit family. In the central section; belongs to the complex I 30 kDa subunit family. In the C-terminal section; belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 794	794	NADH-quinone oxidoreductase subunit B/C/D HAMAP MF_01356		PRO_0000358642
Regions
	Region	1 – 158	158	NADH dehydrogenase I subunit B By similarity
	Region	232 – 387	156	NADH dehydrogenase I subunit C By similarity
	Region	414 – 794	381	NADH dehydrogenase I subunit D By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5E972 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 401E3A17C0EBB7C1	FASTA	794	89,984
	10 20 30 40 50 60 MSQGDDDKIP ENVLLASLDD MINWGRANSL WPMFFGLSCC FVEMMTSFTS RYDVSRFGAE 70 80 90 100 110 120 VLRGTPRESD LMVIAGTVFK KMAPSILRLY DQMAEPKWVI SMGSCANSGG MYDVYSVVQG 130 140 150 160 170 180 VNQILPVDLY IPGCPPRPES FLEGLMLLQQ KIRSEERPTR PVLRMQGGTQ GTVAPILVDG 190 200 210 220 230 240 ATKSRDTRGP GMEGIAIRGS AMQPPYFAAP RSDELWRPKQ PRLPYPDFNL QAELQGAFAG 250 260 270 280 290 300 QVVLDETACD MLTYRAPARL VPELLRHLKE RKESPFRRLE DIACVDESCR RDREKYKDFT 310 320 330 340 350 360 VNYHLTCFDT PGRIRIKTEL EGSYPEAPSI TSVFPVANWY EREAYDMFGI RFAGHPNLRR 370 380 390 400 410 420 ILMPPDWDGH PLRKEHPARA TELPPYTAED ARRQKALPAG DFFDRVDDET LILNLGPQHP 430 440 450 460 470 480 GTHGVIRFVL KLSGEEIVDM DSDIGYHHRA AEKTGERQNW HQYIPYTDRV DYLSGVQNNL 490 500 510 520 530 540 AYLNSVETLC GIEIPDRAIY IRVMLCELFR IANHLVWLGT FASDLGAMTP VFYTFTDREK 550 560 570 580 590 600 IFDIVEFITG GRMHPAWFRI GGVAEDLPEG WQEKVHSFLE WFPGRLAEYE KLLSGNPIFV 610 620 630 640 650 660 ARLKGVSAIT VDTALEWGIT GPNLRACDFA WDLRKKMPYG GYDRFEFEVA TAQGGDCYAR 670 680 690 700 710 720 YQVRMEEMRQ SLSIVRQAAA GMPGGRFISP DYRYTLPQKR DALEDIESLI HHFVNSTRGI 730 740 750 760 770 780 SPPKGECYAP IEGSKGEYGY FAVSDGLHTA YRMRIRTATF PHIQSLPVLS RGWLVSDFLA 790 ILGSLDFVLS DLDR

References

[1]

"Complete sequence of Geobacter bemidjiensis BEM." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Lovley D., Richardson P. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bem / ATCC BAA-1014 / DSM 16622.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001124 Genomic DNA. Translation: ACH37209.1.
RefSeq	YP_002137005.1. NC_011146.1.
3D structure databases
ProteinModelPortal	B5E972.
ModBase	Search...
Protein-protein interaction databases
STRING	B5E972.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6783000.
GenomeReviews	Gene locus Gbem_0178 in contig CP001124_GR.
KEGG	gbm:Gbem_0178.
PATRIC	21984017. VBIGeoBem56306_0176.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG459705.
OMA	PHLQQIP.
ProtClustDB	PRK13292.
Family and domain databases
HAMAP	MF_01356. NDH1_NuoB. Fused. [Tree] MF_01359. NDH1_NuoCD_1. Fused. [Tree]
InterPro	IPR010219. NADH_DH_1_suD. IPR010218. NADH_DH_suC. IPR001135. NADH_Q_OxRdtase_suD. IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR020396. NADH_UbQ_OxRdtase_CS. IPR006138. NADH_UQ_OxRdtase_20Kd_su. IPR022885. NDH1_su_D/H. [Graphical view]
Gene3D	G3DSA:3.40.50.700. G3DSA:3.40.50.700. 1 hit.
KO	K13380.
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 1 hit. PF01058. Oxidored_q6. 1 hit. [Graphical view]
ProDom	PD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01957. NuoB_fam. 1 hit. TIGR01962. NuoD. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. False negative. PS00542. COMPLEX1_30K. 1 hit. PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUBCD_GEOBB

Accession

Primary (citable) accession number: B5E972

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program