B5E5S7 (NADE_STRP4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	SPG_1360

Organism

Streptococcus pneumoniae serotype 19F (strain G54) [Complete proteome] [HAMAP]

Taxonomic identifier

512566 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099049
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5E5S7 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 0CE724FAE1A9044C	FASTA	274	30,195
	10 20 30 40 50 60 MSLQETIIQE LGVKPVIDAQ EEIRRSIDFL KRYLKKHPFL KTFVLGISGG QDSTLAGRLA 70 80 90 100 110 120 QLAMEELRAE TGDDSYKFIA VRLPYGVQAD EADAQKALAF IQPDVSLVVN IKESADAMTA 130 140 150 160 170 180 AVEATGSPVS DFNKGNIKAR CRMIAQYALA GFHSGAVIGT DHAAENITGF FTKFGDGGAD 190 200 210 220 230 240 ILPLYRLNKR QGKQLLQKLG AEPALYEKIP TADLEEDKPG LADEVALGVT YAEIDDYLEG 250 260 270 KTISPEAQAT IENWWHKGQH KRHLPITVFD DFWE

References

[1]

"Annotated draft genomic sequence from a Streptococcus pneumoniae type 19F clinical isolate." Dopazo J., Mendoza A., Herrero J., Caldara F., Humbert Y., Friedli L., Guerrier M., Grand-Schenk E., Gandin C., de Francesco M., Polissi A., Buell G., Feger G., Garcia E., Peitsch M., Garcia-Bustos J.F. Microb. Drug Resist. 7:99-125(2001) [PubMed: 11442348] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: G54.

[2]

"Pneumococcal beta glucoside metabolism investigated by whole genome comparison." Mulas L., Trappetti C., Hakenbeck R., Iannelli F., Pozzi G., Davidsen T.M., Tettelin H., Oggioni M. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: G54.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001015 Genomic DNA. Translation: ACF56514.1.
RefSeq	YP_002038067.1. NC_011072.1.
3D structure databases
ProteinModelPortal	B5E5S7.
SMR	B5E5S7. Positions 2-274.
ModBase	Search...
Protein-protein interaction databases
STRING	B5E5S7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000021424; EBSTRP00000020590; EBSTRG00000021424.
GeneID	6479289.
GenomeReviews	Gene locus SPG_1360 in contig CP001015_GR.
KEGG	spx:SPG_1360.
PATRIC	19688343. VBIStrPne77426_1422.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027750.
HOGENOM	HBG351567.
OMA	IAQYEIA.
ProtClustDB	PRK00768.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_STRP4

Accession

Primary (citable) accession number: B5E5S7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program