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Protein

Hydroxyethylthiazole kinase 2

Gene

thiM2

Organism
Streptococcus pneumoniae serotype 19F (strain G54)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase 1 (thiM1), Hydroxyethylthiazole kinase 2 (thiM2)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411Substrate; via amide nitrogenUniRule annotation
Binding sitei116 – 1161ATPUniRule annotation
Binding sitei166 – 1661ATPUniRule annotation
Binding sitei193 – 1931Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPNE512566:GCA3-657-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinase 2UniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase 2UniRule annotation
Short name:
TH kinase 2UniRule annotation
Short name:
Thz kinase 2UniRule annotation
Gene namesi
Name:thiM2UniRule annotation
Ordered Locus Names:SPG_0657
OrganismiStreptococcus pneumoniae serotype 19F (strain G54)
Taxonomic identifieri512566 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Hydroxyethylthiazole kinase 2PRO_0000383906Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB5E327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiAKPIMAE.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

B5E327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEFTNPFPI GSSSLIHCMT NEISCEMLAN GILALGCKPV MADDPREVLD
60 70 80 90 100
FTKQSQALFI NLGHLSAEKE KAIRIAASYA AQVCLPMVVD AVGVTASSIR
110 120 130 140 150
KSLVRDLLDY RPTVLKGNMS EIRSLVGLKH HGVGVDASAK DQETEDLLQV
160 170 180 190 200
LKDWCQTYHG MSFLVTGPKD LVVSKNQVAV LGNGCAELDW ITGTGDLVGA
210 220 230 240 250
LTAVFLSQGK TGFEASCLAV SYLNIAAEKI VVQGMGLEEF RYQVLNQLSL
260
LRRDENWLDT IKGEVYE
Length:267
Mass (Da):29,085
Last modified:October 14, 2008 - v1
Checksum:i4E0622418EBA4C7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001015 Genomic DNA. Translation: ACF56197.1.
RefSeqiWP_001155186.1. NC_011072.1.
YP_002037372.1. NC_011072.1.

Genome annotation databases

EnsemblBacteriaiACF56197; ACF56197; SPG_0657.
KEGGispx:SPG_0657.
PATRICi19686875. VBIStrPne77426_0697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001015 Genomic DNA. Translation: ACF56197.1.
RefSeqiWP_001155186.1. NC_011072.1.
YP_002037372.1. NC_011072.1.

3D structure databases

ProteinModelPortaliB5E327.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACF56197; ACF56197; SPG_0657.
KEGGispx:SPG_0657.
PATRICi19686875. VBIStrPne77426_0697.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiAKPIMAE.
OrthoDBiEOG628F8M.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciSPNE512566:GCA3-657-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: G54.
  2. "Pneumococcal beta glucoside metabolism investigated by whole genome comparison."
    Mulas L., Trappetti C., Hakenbeck R., Iannelli F., Pozzi G., Davidsen T.M., Tettelin H., Oggioni M.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: G54.

Entry informationi

Entry nameiTHIM2_STRP4
AccessioniPrimary (citable) accession number: B5E327
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: October 14, 2008
Last modified: June 24, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.