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B5E261 (TRMB_STRP4) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:SPG_0497
OrganismStreptococcus pneumoniae serotype 19F (strain G54) [Complete proteome] [HAMAP]
Taxonomic identifier512566 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length211 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 211211tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000136369

Regions

Region124 – 1296Interaction with RNA Potential
Region191 – 1944Substrate binding By similarity

Sites

Active site1181 By similarity
Binding site441S-adenosyl-L-methionine By similarity
Binding site691S-adenosyl-L-methionine By similarity
Binding site961S-adenosyl-L-methionine By similarity
Binding site1181S-adenosyl-L-methionine By similarity
Binding site1221Substrate By similarity
Binding site1541Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B5E261 [UniParc].

Last modified October 14, 2008. Version 1.
Checksum: F9078B8D918F4C95

FASTA21124,341
        10         20         30         40         50         60 
MRVRNRKGAT ELLEANPQYV VLNPLEAKAK WRDLFGNDNP IHVEVGSGKG AFVSGMAKQN 

        70         80         90        100        110        120 
PDINYIGIDI QKSVLSYALD KVLEAGVSNI KLLWVDGSDL TDYFEDGEID RLYLNFSDPW 

       130        140        150        160        170        180 
PKKRHEKRRL TYKTFLDTFK RILPENGEIH FKTDNRGLFE YSLVSFSQYG MKLNGVWLDL 

       190        200        210 
HASDFEGNVM TEYEQKFSNK GQVIYRVEAE F 

« Hide

References

[1]"Annotated draft genomic sequence from a Streptococcus pneumoniae type 19F clinical isolate."
Dopazo J., Mendoza A., Herrero J., Caldara F., Humbert Y., Friedli L., Guerrier M., Grand-Schenk E., Gandin C., de Francesco M., Polissi A., Buell G., Feger G., Garcia E., Peitsch M., Garcia-Bustos J.F.
Microb. Drug Resist. 7:99-125(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: G54.
[2]"Pneumococcal beta glucoside metabolism investigated by whole genome comparison."
Mulas L., Trappetti C., Hakenbeck R., Iannelli F., Pozzi G., Davidsen T.M., Tettelin H., Oggioni M.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: G54.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001015 Genomic DNA. Translation: ACF55826.1.
RefSeqYP_002037212.1. NC_011072.1.

3D structure databases

ProteinModelPortalB5E261.
SMRB5E261. Positions 6-211.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING512566.SPG_0497.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACF55826; ACF55826; SPG_0497.
GeneID6479439.
KEGGspx:SPG_0497.
PATRIC19686524. VBIStrPne77426_0520.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMAINYLEAT.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycSPNE512566:GCA3-497-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_STRP4
AccessionPrimary (citable) accession number: B5E261
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 14, 2008
Last modified: June 11, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways