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B5DFI8

- UBE2S_RAT

UniProt

B5DFI8 - UBE2S_RAT

Protein

Ubiquitin-conjugating enzyme E2 S

Gene

Ube2s

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (14 Oct 2008)
      Previous versions | rss
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    Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei95 – 951Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. activation of anaphase-promoting complex activity Source: UniProtKB
    2. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
    3. exit from mitosis Source: UniProtKB
    4. free ubiquitin chain polymerization Source: UniProtKB
    5. protein K11-linked ubiquitination Source: UniProtKB
    6. protein K27-linked ubiquitination Source: UniProtKB
    7. protein K29-linked ubiquitination Source: UniProtKB
    8. protein K63-linked ubiquitination Source: UniProtKB
    9. protein K6-linked ubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 S (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein S
    Ubiquitin-protein ligase S
    Gene namesi
    Name:Ube2s
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi1564746. Ube2s.

    Subcellular locationi

    GO - Cellular componenti

    1. anaphase-promoting complex Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 223223Ubiquitin-conjugating enzyme E2 SPRO_0000390428Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Post-translational modificationi

    Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.By similarity

    Keywords - PTMi

    Acetylation, Ubl conjugation

    PTM databases

    PhosphoSiteiB5DFI8.

    Interactioni

    Subunit structurei

    Component of the APC/C complex. Interacts with CDC20, FZR1/CDH1 and VHL By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000022977.

    Structurei

    3D structure databases

    ProteinModelPortaliB5DFI8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00730000110565.
    HOGENOMiHOG000233455.
    HOVERGENiHBG063308.
    KOiK10583.
    OMAiPDLGIKH.
    OrthoDBiEOG73JKXD.
    PhylomeDBiB5DFI8.
    TreeFamiTF101120.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B5DFI8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSNVENLPP HIIRLVYKEV TTLTADPPDG IKVFPNEEDL TDLQVTIEGP    50
    EGTPYAGGLF RMKLLLGKDF PASPPKGYFL TKIFHPNVGP NGEICVNVLK 100
    RDWTAELGIR HVLLTIKCLL IHPNPESALN EEAGRLLLEN YEEYAARARL 150
    LTEIHGGACS TSSGRAEASR DLASGASASS TDPMTPGVLG GAEGPMAKKH 200
    AGERDKKLAA KKKLDKKRAL RRL 223
    Length:223
    Mass (Da):24,215
    Last modified:October 14, 2008 - v1
    Checksum:iEB3C7AE437689AD5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH474075 Genomic DNA. Translation: EDL75864.1.
    BC169077 mRNA. Translation: AAI69077.1.
    RefSeqiNP_001099694.1. NM_001106224.2.
    UniGeneiRn.120398.

    Genome annotation databases

    EnsembliENSRNOT00000022977; ENSRNOP00000022977; ENSRNOG00000016930.
    GeneIDi292588.
    KEGGirno:292588.
    UCSCiRGD:1564746. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH474075 Genomic DNA. Translation: EDL75864.1 .
    BC169077 mRNA. Translation: AAI69077.1 .
    RefSeqi NP_001099694.1. NM_001106224.2.
    UniGenei Rn.120398.

    3D structure databases

    ProteinModelPortali B5DFI8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000022977.

    PTM databases

    PhosphoSitei B5DFI8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000022977 ; ENSRNOP00000022977 ; ENSRNOG00000016930 .
    GeneIDi 292588.
    KEGGi rno:292588.
    UCSCi RGD:1564746. rat.

    Organism-specific databases

    CTDi 27338.
    RGDi 1564746. Ube2s.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00730000110565.
    HOGENOMi HOG000233455.
    HOVERGENi HBG063308.
    KOi K10583.
    OMAi PDLGIKH.
    OrthoDBi EOG73JKXD.
    PhylomeDBi B5DFI8.
    TreeFami TF101120.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    NextBioi 634366.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.

    Entry informationi

    Entry nameiUBE2S_RAT
    AccessioniPrimary (citable) accession number: B5DFI8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: October 14, 2008
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3