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Protein

AN1-type zinc finger protein 5

Gene

Zfand5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in protein degradation via the ubiquitin-proteasome system. May act by anchoring ubiquitinated proteins to the proteasome. Plays a role in ubiquitin-mediated protein degradation during muscle atrophy. Plays a role in the regulation of NF-kappa-B activation and apoptosis. Inhibits NF-kappa-B activation triggered by overexpression of RIPK1 and TRAF6 but not of RELA. Inhibits also tumor necrosis factor (TNF), IL-1 and TLR4-induced NF-kappa-B activation in a dose-dependent manner. Overexpression sensitizes cells to TNF-induced apoptosis. Is a potent inhibitory factor for osteoclast differentiation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri8 – 4235A20-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri151 – 19444AN1-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
AN1-type zinc finger protein 5
Alternative name(s):
Zinc finger protein 216
Gene namesi
Name:Zfand5
Synonyms:Zfp216, Znf216
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1310776. Zfand5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213AN1-type zinc finger protein 5PRO_0000415373Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481PhosphoserineBy similarity
Modified residuei58 – 581PhosphoserineBy similarity
Modified residuei209 – 2091N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiB5DF11.
PeptideAtlasiB5DF11.

PTM databases

PhosphoSiteiB5DF11.

Expressioni

Gene expression databases

BgeeiENSRNOG00000018107.
GenevisibleiB5DF11. RN.

Interactioni

Subunit structurei

Homooligomer and/or heterooligomer. Interacts (via A20-type domain) with IKBKG and RIPK1 and with TRAF6 (via AN1-type domain) (By similarity). Interacts with ubiquitin and polyubiquitinated proteins. Identified in a heterotrimeric complex with ubiquitin and SQSTM1, where ZFAND5 and SQSTM1 both interact with the same ubiquitin molecule.By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024583.

Structurei

Secondary structure

1
213
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi19 – 213Combined sources
Turni24 – 274Combined sources
Helixi31 – 4414Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KZYNMR-A1-60[»]
2L00NMR-A1-60[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiB5DF11.

Family & Domainsi

Domaini

The A20-type zinc finger directly binds polyubiquitin chains and associates with the 26S proteasome. The zinc-finger A20-type domain is essential for inhibition of NF-kappa-B activation (By similarity).By similarity

Sequence similaritiesi

Contains 1 A20-type zinc finger.PROSITE-ProRule annotation
Contains 1 AN1-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri8 – 4235A20-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri151 – 19444AN1-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3173. Eukaryota.
ENOG4111UWC. LUCA.
GeneTreeiENSGT00510000046525.
HOGENOMiHOG000238229.
HOVERGENiHBG053349.
InParanoidiB5DF11.
OMAiSLNNCES.
OrthoDBiEOG091G0M53.
PhylomeDBiB5DF11.
TreeFamiTF313612.

Family and domain databases

Gene3Di4.10.1110.10. 1 hit.
InterProiIPR002653. Znf_A20.
IPR000058. Znf_AN1.
[Graphical view]
PfamiPF01754. zf-A20. 1 hit.
PF01428. zf-AN1. 1 hit.
[Graphical view]
SMARTiSM00259. ZnF_A20. 1 hit.
SM00154. ZnF_AN1. 1 hit.
[Graphical view]
PROSITEiPS51036. ZF_A20. 1 hit.
PS51039. ZF_AN1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5DF11-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQETNQTPG PMLCSTGCGF YGNPRTNGMC SVCYKEHLQR QQNSGRMSPM
60 70 80 90 100
GTASGSNSPT SDSASVQRAD ATLNNCEGAA GSTSEKSRNV PVAALPVTQQ
110 120 130 140 150
MTEMSISRED KITSPKTEVS EPVVTQPSPS VSQPSSSQSE EKAPELPKPK
160 170 180 190 200
KNRCFMCRKK VGLTGFDCRC GNLFCGLHRY SDKHNCPYDY KAEAAAKIRK
210
ENPVVVAEKI QRI
Length:213
Mass (Da):23,088
Last modified:October 14, 2008 - v1
Checksum:iB293DB1A55E79B9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473953 Genomic DNA. Translation: EDM13001.1.
BC168882 mRNA. Translation: AAI68882.1.
RefSeqiXP_008758519.1. XM_008760297.1.
UniGeneiRn.4050.

Genome annotation databases

EnsembliENSRNOT00000024583; ENSRNOP00000024583; ENSRNOG00000018107.
GeneIDi293960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473953 Genomic DNA. Translation: EDM13001.1.
BC168882 mRNA. Translation: AAI68882.1.
RefSeqiXP_008758519.1. XM_008760297.1.
UniGeneiRn.4050.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KZYNMR-A1-60[»]
2L00NMR-A1-60[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024583.

PTM databases

PhosphoSiteiB5DF11.

Proteomic databases

PaxDbiB5DF11.
PeptideAtlasiB5DF11.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024583; ENSRNOP00000024583; ENSRNOG00000018107.
GeneIDi293960.

Organism-specific databases

CTDi7763.
RGDi1310776. Zfand5.

Phylogenomic databases

eggNOGiKOG3173. Eukaryota.
ENOG4111UWC. LUCA.
GeneTreeiENSGT00510000046525.
HOGENOMiHOG000238229.
HOVERGENiHBG053349.
InParanoidiB5DF11.
OMAiSLNNCES.
OrthoDBiEOG091G0M53.
PhylomeDBiB5DF11.
TreeFamiTF313612.

Miscellaneous databases

EvolutionaryTraceiB5DF11.
PROiB5DF11.

Gene expression databases

BgeeiENSRNOG00000018107.
GenevisibleiB5DF11. RN.

Family and domain databases

Gene3Di4.10.1110.10. 1 hit.
InterProiIPR002653. Znf_A20.
IPR000058. Znf_AN1.
[Graphical view]
PfamiPF01754. zf-A20. 1 hit.
PF01428. zf-AN1. 1 hit.
[Graphical view]
SMARTiSM00259. ZnF_A20. 1 hit.
SM00154. ZnF_AN1. 1 hit.
[Graphical view]
PROSITEiPS51036. ZF_A20. 1 hit.
PS51039. ZF_AN1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZFAN5_RAT
AccessioniPrimary (citable) accession number: B5DF11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: October 14, 2008
Last modified: September 7, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.