B5DEQ3 (XPP3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable Xaa-Pro aminopeptidase 3
Short name=X-Pro aminopeptidase 3
EC=3.4.11.9

Alternative name(s):
Aminopeptidase P3
Short name=APP3

Gene names

Name:

Xpnpep3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Mitochondrion By similarity.
Tissue specificity	Expressed in the kidney, specifically in intercalated cells, but not in principal cells, of the distal convoluted tubule and cortical collecting duct (at protein level). Ref.2
Sequence similarities	Belongs to the peptidase M24B family.

Ontologies

Keywords
Cellular component	Mitochondrion
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular process Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Probable Xaa-Pro aminopeptidase 3		PRO_0000401209
Sites
	Metal binding	331	1	Manganese 2 By similarity
	Metal binding	342	1	Manganese 1 By similarity
	Metal binding	342	1	Manganese 2 By similarity
	Metal binding	423	1	Manganese 1 By similarity
	Metal binding	450	1	Manganese 1 By similarity
	Metal binding	474	1	Manganese 1 By similarity
	Metal binding	474	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5DEQ3 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 6B793744D67B09F1	FASTA	506	56,455
	10 20 30 40 50 60 MLSLLSTPRL VPVIARLRGL SGCMSCLQRR YSLQPVPVKE IPNRYLGQPS PVTHPHLLRP 70 80 90 100 110 120 GEVTPGLSQV EYALRRHKLM ALVHKEAQGH SGTDHTVVVL SNPIHYMSND IPYTFHQDNS 130 140 150 160 170 180 FLYLCGFQEP DSILVLQSCS GKQLPSHKAM LFVPRRDPGR ELWDGPRSGT DGAIALTGVD 190 200 210 220 230 240 DAYPLEEFQH LLPKLRAETN MVWYDWMKPS HAQLHSDYMQ PLTEAKATSK NKVRSVQHLI 250 260 270 280 290 300 QHLRLIKSPA EIKRMQIAGK LTSEAFIETM FASKAPVDEA FLYAKFEFEC RARGADILAY 310 320 330 340 350 360 PPVVAGGNRS NTLHYVKNNQ LIKDGEMVLL DGGCESSCYV SDITRTWPVN GRFTAPQAEL 370 380 390 400 410 420 YEAVLEIQKA CLTLCSPGTS LENIYSMMLT LMGQKLKDLG IIKTSKESAF KAARKYCPHH 430 440 450 460 470 480 VGHYLGMDVH DTPDMPRSLP LQPGMVITVE PGIYIPEGDT DAPEKFRGLG VRIEDDVVVT 490 500 QDSPLILSAD CPKEVNDIEQ ICSRTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[2]	"Individuals with mutations in XPNPEP3, which encodes a mitochondrial protein, develop a nephronophthisis-like nephropathy." O'Toole J.F., Liu Y., Davis E.E., Westlake C.J., Attanasio M., Otto E.A., Seelow D., Nurnberg G., Becker C., Nuutinen M., Karppa M., Ignatius J., Uusimaa J., Pakanen S., Jaakkola E., van den Heuvel L.P., Fehrenbach H., Wiggins R. , Goyal M., Zhou W., Wolf M.T., Wise E., Helou J., Allen S.J., Murga-Zamalloa C.A., Ashraf S., Chaki M., Heeringa S., Chernin G., Hoskins B.E., Chaib H., Gleeson J., Kusakabe T., Suzuki T., Isaac R.E., Quarmby L.M., Tennant B., Fujioka H., Tuominen H., Hassinen I., Lohi H., van Houten J.L., Rotig A., Sayer J.A., Rolinski B., Freisinger P., Madhavan S.M., Herzer M., Madignier F., Prokisch H., Nurnberg P., Jackson P.K., Khanna H., Katsanis N., Hildebrandt F. J. Clin. Invest. 120:791-802(2010) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[3]	Erratum O'Toole J.F., Liu Y., Davis E.E., Westlake C.J., Attanasio M., Otto E.A., Seelow D., Nurnberg G., Becker C., Nuutinen M., Karppa M., Ignatius J., Uusimaa J., Pakanen S., Jaakkola E., van den Heuvel L.P., Fehrenbach H., Wiggins R. , Goyal M., Zhou W., Wolf M.T., Wise E., Helou J., Allen S.J., Murga-Zamalloa C.A., Ashraf S., Chaki M., Heeringa S., Chernin G., Hoskins B.E., Chaib H., Gleeson J., Kusakabe T., Suzuki T., Isaac R.E., Quarmby L.M., Tennant B., Fujioka H., Tuominen H., Hassinen I., Lohi H., van Houten J.L., Rotig A., Sayer J.A., Rolinski B., Freisinger P., Madhavan S.M., Herzer M., Madignier F., Prokisch H., Nurnberg P., Jackson P.K., Khanna H., Katsanis N., Hildebrandt F. J. Clin. Invest. 120:1362-1362(2010)

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC168759 mRNA. Translation: AAI68759.1.
IPI	IPI00361438.
RefSeq	NP_001124054.2. NM_001130582.2.
UniGene	Rn.224672.
3D structure databases
ProteinModelPortal	B5DEQ3.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000061709.
Protein family/group databases
MEROPS	M24.026.
PTM databases
PhosphoSite	B5DEQ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	685823.
KEGG	rno:685823.
UCSC	RGD:1589063. rat.
Organism-specific databases
CTD	63929.
RGD	1589063. Xpnpep3.
Phylogenomic databases
eggNOG	COG0006.
HOGENOM	HOG000008762.
HOVERGEN	HBG057305.
KO	K01262.
OrthoDB	EOG4KPT9J.
Gene expression databases
Genevestigator	B5DEQ3.
Family and domain databases
Gene3D	3.90.230.10. 1 hit.
InterPro	IPR007865. Aminopep_P_N. IPR000994. Pept_M24_structural-domain. [Graphical view]
Pfam	PF05195. AMP_N. 1 hit. PF00557. Peptidase_M24. 1 hit. [Graphical view]
SMART	SM01011. AMP_N. 1 hit. [Graphical view]
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
ProtoNet	Search...
Other
NextBio	730541.

Entry information

Entry name

XPP3_RAT

Accession

Primary (citable) accession number: B5DEQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 30, 2010
Last sequence update:	October 14, 2008
Last modified:	April 3, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program