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Protein

Xaa-Pro aminopeptidase 3

Gene

Xpnpep3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Leu-Pro-Ala. Also shows low activity towards peptides with Ala or Ser at the P1 position. Promotes TNFRSF1B-mediated phosphorylation of MAPK8/JNK1 and MAPK9/JNK2, suggesting a function as an adapter protein for TNFRSF1B; the effect is independent of XPNPEP3 peptidase activity. May inhibit apoptotic cell death induced via TNF-TNFRSF1B signaling.By similarity

Catalytic activityi

Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.By similarity

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei300SubstrateBy similarity1
Metal bindingi331Manganese 2By similarity1
Binding sitei331SubstrateBy similarity1
Metal bindingi342Manganese 1By similarity1
Metal bindingi342Manganese 2By similarity1
Binding sitei342SubstrateBy similarity1
Metal bindingi423Manganese 1By similarity1
Binding sitei423SubstrateBy similarity1
Binding sitei430SubstrateBy similarity1
Metal bindingi450Manganese 1By similarity1
Binding sitei450SubstrateBy similarity1
Metal bindingi474Manganese 1By similarity1
Metal bindingi474Manganese 2By similarity1
Binding sitei474SubstrateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
LigandManganese, Metal-binding

Protein family/group databases

MEROPSiM24.026

Names & Taxonomyi

Protein namesi
Recommended name:
Xaa-Pro aminopeptidase 3Imported (EC:3.4.11.9)
Short name:
X-Pro aminopeptidase 3
Alternative name(s):
Aminopeptidase P3
Short name:
APP3
Gene namesi
Name:Xpnpep3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1589063 Xpnpep3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 31MitochondrionSequence analysisAdd BLAST31
ChainiPRO_000040120932 – 506Xaa-Pro aminopeptidase 3Add BLAST475

Proteomic databases

PaxDbiB5DEQ3
PeptideAtlasiB5DEQ3
PRIDEiB5DEQ3

PTM databases

iPTMnetiB5DEQ3
PhosphoSitePlusiB5DEQ3

Expressioni

Tissue specificityi

Expressed in the kidney, specifically in intercalated cells, but not in principal cells, of the distal convoluted tubule and cortical collecting duct (at protein level).1 Publication

Interactioni

Subunit structurei

Homodimer. Interacts with TNFRSF1B/TNFR2 (activated) and TRAF2.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061709

Structurei

3D structure databases

ProteinModelPortaliB5DEQ3
SMRiB5DEQ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 79Interaction with TNFRSF1BBy similarityAdd BLAST26

Sequence similaritiesi

Belongs to the peptidase M24B family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2414 Eukaryota
COG0006 LUCA
HOGENOMiHOG000008762
HOVERGENiHBG057305
InParanoidiB5DEQ3
KOiK01262
PhylomeDBiB5DEQ3

Family and domain databases

Gene3Di3.40.350.10, 1 hit
InterProiView protein in InterPro
IPR007865 Aminopep_P_N
IPR029149 Creatin/AminoP/Spt16_NTD
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
PfamiView protein in Pfam
PF05195 AMP_N, 1 hit
PF00557 Peptidase_M24, 1 hit
SMARTiView protein in SMART
SM01011 AMP_N, 1 hit
SUPFAMiSSF53092 SSF53092, 1 hit
SSF55920 SSF55920, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B5DEQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSLLSTPRL VPVIARLRGL SGCMSCLQRR YSLQPVPVKE IPNRYLGQPS
60 70 80 90 100
PVTHPHLLRP GEVTPGLSQV EYALRRHKLM ALVHKEAQGH SGTDHTVVVL
110 120 130 140 150
SNPIHYMSND IPYTFHQDNS FLYLCGFQEP DSILVLQSCS GKQLPSHKAM
160 170 180 190 200
LFVPRRDPGR ELWDGPRSGT DGAIALTGVD DAYPLEEFQH LLPKLRAETN
210 220 230 240 250
MVWYDWMKPS HAQLHSDYMQ PLTEAKATSK NKVRSVQHLI QHLRLIKSPA
260 270 280 290 300
EIKRMQIAGK LTSEAFIETM FASKAPVDEA FLYAKFEFEC RARGADILAY
310 320 330 340 350
PPVVAGGNRS NTLHYVKNNQ LIKDGEMVLL DGGCESSCYV SDITRTWPVN
360 370 380 390 400
GRFTAPQAEL YEAVLEIQKA CLTLCSPGTS LENIYSMMLT LMGQKLKDLG
410 420 430 440 450
IIKTSKESAF KAARKYCPHH VGHYLGMDVH DTPDMPRSLP LQPGMVITVE
460 470 480 490 500
PGIYIPEGDT DAPEKFRGLG VRIEDDVVVT QDSPLILSAD CPKEVNDIEQ

ICSRTS
Length:506
Mass (Da):56,455
Last modified:October 14, 2008 - v1
Checksum:i6B793744D67B09F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC168759 mRNA Translation: AAI68759.1
RefSeqiNP_001124054.2, NM_001130582.2
UniGeneiRn.224672

Genome annotation databases

GeneIDi685823
KEGGirno:685823
UCSCiRGD:1589063 rat

Similar proteinsi

Entry informationi

Entry nameiXPP3_RAT
AccessioniPrimary (citable) accession number: B5DEQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: October 14, 2008
Last modified: May 23, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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