B5DE93 (CDK12_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 12
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cell division protein kinase 12

Gene names

Name:

cdk12

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	1239 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit rpb1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, possibly by phosphorylating srsf1/sf2 By similarity.
Catalytic activity	ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate. ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Nucleus By similarity. Nucleus speckle By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA splicing Inferred from sequence or structural similarity. Source: UniProtKB cell cycle Inferred from electronic annotation. Source: GOC mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW phosphorylation of RNA polymerase II C-terminal domain Inferred from sequence or structural similarity. Source: UniProtKB regulation of MAP kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nuclear cyclin-dependent protein kinase holoenzyme complex Inferred from sequence or structural similarity. Source: UniProtKB nuclear speck Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from sequence or structural similarity. Source: UniProtKB cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1239	1239	Cyclin-dependent kinase 12		PRO_0000406959
Regions
	Domain	710 – 1003	294	Protein kinase
	Nucleotide binding	716 – 724	9	ATP By similarity
	Compositional bias	163 – 383	221	Ser-rich
	Compositional bias	396 – 402	7	Poly-Ala
	Compositional bias	507 – 690	184	Pro-rich
	Compositional bias	1214 – 1218	5	Poly-Glu
	Compositional bias	1227 – 1231	5	Poly-Glu
Sites
	Active site	842	1	Proton acceptor By similarity
	Binding site	739	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5DE93 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 8ADF1CECFCE9CB12	FASTA	1,239	138,328
	10 20 30 40 50 60 MPNPVRHGNK KDSGGSASTM PESSGSVNNK DRQRISSKHK RHKSKHLRES SVPEPQTLST 70 80 90 100 110 120 LKLVEYDDIS SDSDTFSDVP AKQERRENDE RRPSEKSDKM HKSRHRHQHK RLKEATRTKQ 130 140 150 160 170 180 VEKERKPEKT QEVMVNKVPS SKDKIISSAK RTADEQEEHG RTSKSSSKDS RSKMHKDKSR 190 200 210 220 230 240 REREMKSGHK ERSKSHRKRD SSKRYKTSDS PKRKGRSPRR KYGGSPKQDD SHSEGSCGQD 250 260 270 280 290 300 YDDSPSRVHA SGNFNETYRK NMDSPSYKEP AAYQSSARSP SPYSRKQRSV SPYRRRSSSY 310 320 330 340 350 360 ERGSGSYSGR SPSPYVRRRS SSPFVSRRSL SRSPAPRKSA KSRSRSPLYP RKSSSSSRSK 370 380 390 400 410 420 KQKSQSRSRH SSISPARLPL NSSLGAELSR KKKEIAAAAA AAGRESKGSP LILKKESGDS 430 440 450 460 470 480 RVASALDLKK ATKVLKLEKP VSEPEMVTAT SSETKTISAK KEESEKKPQV QAKDPKISGL 490 500 510 520 530 540 KDCKPAAIKE EVLTPKEGVT DRDKDVPPLP PILSPPPPLP TSPPSNIPPL PPLPPSPAVL 550 560 570 580 590 600 QQPPPPLPPP PTLTFLPLPT TVAASLTSSH YVRQSSLSTQ ANSQLPVPSP AKPQPPLIHM 610 620 630 640 650 660 VPHMKTSTLP PLPLPPLLLG EDDIESPKEI PPPKSVKKEK DKDRPRHLLT DLPLPPELPG 670 680 690 700 710 720 GDPSPPDSPE RKVVAPPQPP LKKRPKICCP RYGERKQTET DWGKRCVDKF DIIGIIGEGT 730 740 750 760 770 780 YGQVYKAKDK DTGELVALKK VRLDNEKEGF PITAIREIKI LRQLIHKSVV NMKEIVTDKQ 790 800 810 820 830 840 DALDFKKDKG AFYLVFEYMD HDLMGLLESG LVQFSEDHIK SFMKQLMEGL EYCHKKNFLH 850 860 870 880 890 900 RDIKCSNILL NNSGQIKLAD FGLARLYSSE ESRPYTNKVI TLWYRPPELL LGEERYTPAI 910 920 930 940 950 960 DVWSCGCILG ELFTKKPIFQ ANQELAQLEL ISRLCGSPCP AVWPDVIKLP YFNTMKPKKQ 970 980 990 1000 1010 1020 YRRRLREEFS FVPTPALDLL DHMLTLDPSK RCTAEQTLQS DFLKDVDVCK MATPDLPHWQ 1030 1040 1050 1060 1070 1080 DCHELWSKKR RRQRQSGIVL EEPPAIKAPR KESVSVPGGD AMKNSSPAPT QPVKTESSVS 1090 1100 1110 1120 1130 1140 DLAVLGDITQ QLNQSELAVL LNLLQSQTDL SVSQISQLLN VQANPDMQLQ LEALNQSITA 1150 1160 1170 1180 1190 1200 LTGAAVPPQE VEPPKETPTL DQVDPLGAGT QGEVQNVLAV LLSQLLKPQE VVDAPGESNG 1210 1220 1230 EQSGSNPTAH ITLEEEEEPD KHHEQNEEEE EDAGNYWSP

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC168577 mRNA. Translation: AAI68577.1.
RefSeq	NP_001128283.1. NM_001134811.1.
UniGene	Str.6962.
3D structure databases
ProteinModelPortal	B5DE93.
ModBase	Search...
Protein-protein interaction databases
STRING	8364.ENSXETP00000051128.
Proteomic databases
PRIDE	B5DE93.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100038049.
KEGG	xtr:100038049.
Organism-specific databases
CTD	51755.
Xenbase	XB-GENE-493308. cdk12.
Phylogenomic databases
eggNOG	COG0515.
HOVERGEN	HBG050852.
KO	K08819.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDK12_XENTR

Accession

Primary (citable) accession number: B5DE93

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 5, 2011
Last sequence update:	October 14, 2008
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program