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Protein

Cyclin-dependent kinase 12

Gene

cdk12

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating srsf1/sf2 (By similarity).By similarity

Catalytic activityi

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei739 – 7391ATPPROSITE-ProRule annotation
Active sitei842 – 8421Proton acceptorPROSITE-ProRule annotation
Binding sitei1023 – 10231ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi716 – 7249ATPPROSITE-ProRule annotation
Nucleotide bindingi797 – 8026ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. phosphorylation of RNA polymerase II C-terminal domain Source: UniProtKB
  3. regulation of MAP kinase activity Source: UniProtKB
  4. RNA splicing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 12 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cell division protein kinase 12
Gene namesi
Name:cdk12
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143 Componenti: Unplaced

Organism-specific databases

XenbaseiXB-GENE-493308. cdk12.

Subcellular locationi

Nucleus By similarity. Nucleus speckle By similarity

GO - Cellular componenti

  1. nuclear cyclin-dependent protein kinase holoenzyme complex Source: UniProtKB
  2. nuclear speck Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12391239Cyclin-dependent kinase 12PRO_0000406959Add
BLAST

Proteomic databases

PRIDEiB5DE93.

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000051128.

Structurei

3D structure databases

ProteinModelPortaliB5DE93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini710 – 1003294Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi163 – 383221Ser-richAdd
BLAST
Compositional biasi396 – 4027Poly-Ala
Compositional biasi507 – 690184Pro-richAdd
BLAST
Compositional biasi1214 – 12185Poly-Glu
Compositional biasi1227 – 12315Poly-Glu

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOVERGENiHBG050852.
InParanoidiB5DE93.
KOiK08819.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5DE93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNPVRHGNK KDSGGSASTM PESSGSVNNK DRQRISSKHK RHKSKHLRES
60 70 80 90 100
SVPEPQTLST LKLVEYDDIS SDSDTFSDVP AKQERRENDE RRPSEKSDKM
110 120 130 140 150
HKSRHRHQHK RLKEATRTKQ VEKERKPEKT QEVMVNKVPS SKDKIISSAK
160 170 180 190 200
RTADEQEEHG RTSKSSSKDS RSKMHKDKSR REREMKSGHK ERSKSHRKRD
210 220 230 240 250
SSKRYKTSDS PKRKGRSPRR KYGGSPKQDD SHSEGSCGQD YDDSPSRVHA
260 270 280 290 300
SGNFNETYRK NMDSPSYKEP AAYQSSARSP SPYSRKQRSV SPYRRRSSSY
310 320 330 340 350
ERGSGSYSGR SPSPYVRRRS SSPFVSRRSL SRSPAPRKSA KSRSRSPLYP
360 370 380 390 400
RKSSSSSRSK KQKSQSRSRH SSISPARLPL NSSLGAELSR KKKEIAAAAA
410 420 430 440 450
AAGRESKGSP LILKKESGDS RVASALDLKK ATKVLKLEKP VSEPEMVTAT
460 470 480 490 500
SSETKTISAK KEESEKKPQV QAKDPKISGL KDCKPAAIKE EVLTPKEGVT
510 520 530 540 550
DRDKDVPPLP PILSPPPPLP TSPPSNIPPL PPLPPSPAVL QQPPPPLPPP
560 570 580 590 600
PTLTFLPLPT TVAASLTSSH YVRQSSLSTQ ANSQLPVPSP AKPQPPLIHM
610 620 630 640 650
VPHMKTSTLP PLPLPPLLLG EDDIESPKEI PPPKSVKKEK DKDRPRHLLT
660 670 680 690 700
DLPLPPELPG GDPSPPDSPE RKVVAPPQPP LKKRPKICCP RYGERKQTET
710 720 730 740 750
DWGKRCVDKF DIIGIIGEGT YGQVYKAKDK DTGELVALKK VRLDNEKEGF
760 770 780 790 800
PITAIREIKI LRQLIHKSVV NMKEIVTDKQ DALDFKKDKG AFYLVFEYMD
810 820 830 840 850
HDLMGLLESG LVQFSEDHIK SFMKQLMEGL EYCHKKNFLH RDIKCSNILL
860 870 880 890 900
NNSGQIKLAD FGLARLYSSE ESRPYTNKVI TLWYRPPELL LGEERYTPAI
910 920 930 940 950
DVWSCGCILG ELFTKKPIFQ ANQELAQLEL ISRLCGSPCP AVWPDVIKLP
960 970 980 990 1000
YFNTMKPKKQ YRRRLREEFS FVPTPALDLL DHMLTLDPSK RCTAEQTLQS
1010 1020 1030 1040 1050
DFLKDVDVCK MATPDLPHWQ DCHELWSKKR RRQRQSGIVL EEPPAIKAPR
1060 1070 1080 1090 1100
KESVSVPGGD AMKNSSPAPT QPVKTESSVS DLAVLGDITQ QLNQSELAVL
1110 1120 1130 1140 1150
LNLLQSQTDL SVSQISQLLN VQANPDMQLQ LEALNQSITA LTGAAVPPQE
1160 1170 1180 1190 1200
VEPPKETPTL DQVDPLGAGT QGEVQNVLAV LLSQLLKPQE VVDAPGESNG
1210 1220 1230
EQSGSNPTAH ITLEEEEEPD KHHEQNEEEE EDAGNYWSP
Length:1,239
Mass (Da):138,328
Last modified:October 13, 2008 - v1
Checksum:i8ADF1CECFCE9CB12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC168577 mRNA. Translation: AAI68577.1.
RefSeqiNP_001128283.1. NM_001134811.1.
UniGeneiStr.6962.

Genome annotation databases

GeneIDi100038049.
KEGGixtr:100038049.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC168577 mRNA. Translation: AAI68577.1.
RefSeqiNP_001128283.1. NM_001134811.1.
UniGeneiStr.6962.

3D structure databases

ProteinModelPortaliB5DE93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8364.ENSXETP00000051128.

Proteomic databases

PRIDEiB5DE93.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100038049.
KEGGixtr:100038049.

Organism-specific databases

CTDi51755.
XenbaseiXB-GENE-493308. cdk12.

Phylogenomic databases

eggNOGiCOG0515.
HOVERGENiHBG050852.
InParanoidiB5DE93.
KOiK08819.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiCDK12_XENTR
AccessioniPrimary (citable) accession number: B5DE93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2011
Last sequence update: October 13, 2008
Last modified: January 6, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.