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B5DE93

- CDK12_XENTR

UniProt

B5DE93 - CDK12_XENTR

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Protein
Cyclin-dependent kinase 12
Gene
cdk12
Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating srsf1/sf2 By similarity.

Catalytic activityi

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei739 – 7391ATP By similarity
Active sitei842 – 8421Proton acceptor By similarity
Binding sitei1023 – 10231ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi716 – 7249ATP By similarity
Nucleotide bindingi797 – 8026ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB
  3. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. RNA splicing Source: UniProtKB
  2. mRNA processing Source: UniProtKB-KW
  3. phosphorylation of RNA polymerase II C-terminal domain Source: UniProtKB
  4. regulation of MAP kinase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 12 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cell division protein kinase 12
Gene namesi
Name:cdk12
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143: Unplaced

Organism-specific databases

XenbaseiXB-GENE-493308. cdk12.

Subcellular locationi

Nucleus By similarity. Nucleus speckle By similarity

GO - Cellular componenti

  1. nuclear cyclin-dependent protein kinase holoenzyme complex Source: UniProtKB
  2. nuclear speck Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12391239Cyclin-dependent kinase 12
PRO_0000406959Add
BLAST

Proteomic databases

PRIDEiB5DE93.

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000051128.

Structurei

3D structure databases

ProteinModelPortaliB5DE93.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini710 – 1003294Protein kinase
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi163 – 383221Ser-rich
Add
BLAST
Compositional biasi396 – 4027Poly-Ala
Compositional biasi507 – 690184Pro-rich
Add
BLAST
Compositional biasi1214 – 12185Poly-Glu
Compositional biasi1227 – 12315Poly-Glu

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOVERGENiHBG050852.
KOiK08819.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5DE93-1 [UniParc]FASTAAdd to Basket

« Hide

MPNPVRHGNK KDSGGSASTM PESSGSVNNK DRQRISSKHK RHKSKHLRES     50
SVPEPQTLST LKLVEYDDIS SDSDTFSDVP AKQERRENDE RRPSEKSDKM 100
HKSRHRHQHK RLKEATRTKQ VEKERKPEKT QEVMVNKVPS SKDKIISSAK 150
RTADEQEEHG RTSKSSSKDS RSKMHKDKSR REREMKSGHK ERSKSHRKRD 200
SSKRYKTSDS PKRKGRSPRR KYGGSPKQDD SHSEGSCGQD YDDSPSRVHA 250
SGNFNETYRK NMDSPSYKEP AAYQSSARSP SPYSRKQRSV SPYRRRSSSY 300
ERGSGSYSGR SPSPYVRRRS SSPFVSRRSL SRSPAPRKSA KSRSRSPLYP 350
RKSSSSSRSK KQKSQSRSRH SSISPARLPL NSSLGAELSR KKKEIAAAAA 400
AAGRESKGSP LILKKESGDS RVASALDLKK ATKVLKLEKP VSEPEMVTAT 450
SSETKTISAK KEESEKKPQV QAKDPKISGL KDCKPAAIKE EVLTPKEGVT 500
DRDKDVPPLP PILSPPPPLP TSPPSNIPPL PPLPPSPAVL QQPPPPLPPP 550
PTLTFLPLPT TVAASLTSSH YVRQSSLSTQ ANSQLPVPSP AKPQPPLIHM 600
VPHMKTSTLP PLPLPPLLLG EDDIESPKEI PPPKSVKKEK DKDRPRHLLT 650
DLPLPPELPG GDPSPPDSPE RKVVAPPQPP LKKRPKICCP RYGERKQTET 700
DWGKRCVDKF DIIGIIGEGT YGQVYKAKDK DTGELVALKK VRLDNEKEGF 750
PITAIREIKI LRQLIHKSVV NMKEIVTDKQ DALDFKKDKG AFYLVFEYMD 800
HDLMGLLESG LVQFSEDHIK SFMKQLMEGL EYCHKKNFLH RDIKCSNILL 850
NNSGQIKLAD FGLARLYSSE ESRPYTNKVI TLWYRPPELL LGEERYTPAI 900
DVWSCGCILG ELFTKKPIFQ ANQELAQLEL ISRLCGSPCP AVWPDVIKLP 950
YFNTMKPKKQ YRRRLREEFS FVPTPALDLL DHMLTLDPSK RCTAEQTLQS 1000
DFLKDVDVCK MATPDLPHWQ DCHELWSKKR RRQRQSGIVL EEPPAIKAPR 1050
KESVSVPGGD AMKNSSPAPT QPVKTESSVS DLAVLGDITQ QLNQSELAVL 1100
LNLLQSQTDL SVSQISQLLN VQANPDMQLQ LEALNQSITA LTGAAVPPQE 1150
VEPPKETPTL DQVDPLGAGT QGEVQNVLAV LLSQLLKPQE VVDAPGESNG 1200
EQSGSNPTAH ITLEEEEEPD KHHEQNEEEE EDAGNYWSP 1239
Length:1,239
Mass (Da):138,328
Last modified:October 14, 2008 - v1
Checksum:i8ADF1CECFCE9CB12
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC168577 mRNA. Translation: AAI68577.1.
RefSeqiNP_001128283.1. NM_001134811.1.
UniGeneiStr.6962.

Genome annotation databases

GeneIDi100038049.
KEGGixtr:100038049.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC168577 mRNA. Translation: AAI68577.1 .
RefSeqi NP_001128283.1. NM_001134811.1.
UniGenei Str.6962.

3D structure databases

ProteinModelPortali B5DE93.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 8364.ENSXETP00000051128.

Proteomic databases

PRIDEi B5DE93.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100038049.
KEGGi xtr:100038049.

Organism-specific databases

CTDi 51755.
Xenbasei XB-GENE-493308. cdk12.

Phylogenomic databases

eggNOGi COG0515.
HOVERGENi HBG050852.
KOi K08819.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiCDK12_XENTR
AccessioniPrimary (citable) accession number: B5DE93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 14, 2008
Last modified: June 11, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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