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Protein

Beta-porphyranase A

Gene

BACPLE_01693

Organism
Bacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves the sulfated polysaccharide porphyran at the (1->4) linkages between beta-D-galactopyranose and alpha-L-galactopyranose-6-sulfate, forming mostly the disaccharide alpha-L-galactopyranose-6-sulfate-(1->3)-beta-D-galactose. Some longer oligosaccharides of even number of residues are also observed. Inactive on the non-sulfated agarose portion of the porphyran backbone.1 Publication

Catalytic activityi

Hydrolysis of beta-D-galactopyranose-(1->4)-alpha-L-galactopyranose-6-sulfate linkages in porphyran.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53Substrate1
Binding sitei87Substrate1
Binding sitei114Substrate1
Binding sitei156Substrate1
Binding sitei237Substrate1
Binding sitei324Substrate1
Binding sitei331Substrate1
Binding sitei342Substrate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH86. Glycoside Hydrolase Family 86.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-porphyranase A (EC:3.2.1.178)
Alternative name(s):
Glycosyl hydrolase 86 family protein A
Short name:
GH86A
Gene namesi
ORF Names:BACPLE_01693
OrganismiBacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
Taxonomic identifieri484018 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000003452 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000042203423 – 728Beta-porphyranase AAdd BLAST706

Interactioni

Protein-protein interaction databases

STRINGi484018.BACPLE_01693.

Structurei

Secondary structure

1728
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 38Combined sources9
Helixi46 – 48Combined sources3
Beta strandi50 – 53Combined sources4
Helixi60 – 69Combined sources10
Beta strandi75 – 77Combined sources3
Helixi80 – 88Combined sources9
Beta strandi105 – 112Combined sources8
Turni117 – 119Combined sources3
Helixi126 – 138Combined sources13
Beta strandi141 – 148Combined sources8
Beta strandi150 – 152Combined sources3
Helixi154 – 156Combined sources3
Helixi166 – 185Combined sources20
Turni188 – 193Combined sources6
Beta strandi195 – 202Combined sources8
Turni206 – 211Combined sources6
Helixi212 – 216Combined sources5
Helixi218 – 225Combined sources8
Helixi226 – 228Combined sources3
Beta strandi230 – 240Combined sources11
Helixi252 – 269Combined sources18
Beta strandi275 – 284Combined sources10
Turni294 – 299Combined sources6
Helixi300 – 312Combined sources13
Helixi313 – 317Combined sources5
Beta strandi318 – 324Combined sources7
Helixi330 – 332Combined sources3
Helixi335 – 337Combined sources3
Beta strandi346 – 350Combined sources5
Turni355 – 357Combined sources3
Beta strandi360 – 362Combined sources3
Helixi366 – 372Combined sources7
Beta strandi377 – 386Combined sources10
Beta strandi389 – 396Combined sources8
Beta strandi399 – 406Combined sources8
Beta strandi408 – 410Combined sources3
Beta strandi412 – 417Combined sources6
Turni419 – 424Combined sources6
Beta strandi425 – 436Combined sources12
Turni437 – 439Combined sources3
Beta strandi440 – 450Combined sources11
Beta strandi453 – 457Combined sources5
Beta strandi461 – 469Combined sources9
Beta strandi476 – 484Combined sources9
Beta strandi489 – 491Combined sources3
Beta strandi498 – 505Combined sources8
Beta strandi509 – 521Combined sources13
Beta strandi529 – 532Combined sources4
Beta strandi535 – 537Combined sources3
Beta strandi551 – 562Combined sources12
Helixi564 – 566Combined sources3
Beta strandi569 – 577Combined sources9
Beta strandi583 – 596Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AW7X-ray1.33A30-598[»]
ProteinModelPortaliB5CY96.
SMRiB5CY96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini599 – 701CBM-cenCAdd BLAST103

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni77 – 78Substrate binding2
Regioni151 – 152Substrate binding2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 86 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108KBQ. Bacteria.
ENOG410XSB9. LUCA.
OrthoDBiPOG091H0A4S.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR003305. CenC_carb-bd.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02018. CBM_4_9. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B5CY96-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYKYIFLLS AFTLGVPPGI YCQGRNEVVV DYNTRRFLSG VSELDRSKYF
60 70 80 90 100
NIHSTSDDDK DVGKFLADYQ VGLGRKFWGP YSYAYNKTHE VGKYPQMKPY
110 120 130 140 150
SGNISVKRYI ATEHPYVQHI QGGIDVQAAG AWSAEYYSNS ELVPEFFEPL
160 170 180 190 200
NEPFVHANDA GFTVQGQAMR ELMVDFYASI GKHIHNNPRL NGKMKVIGYA
210 220 230 240 250
AAYPAWEDGN FNYWNTRMKM FIDRAGAYMD GFSVHLYDGI NVTGTDTKRS
260 270 280 290 300
GSNSEAVLDM VEAYSYIKFG HVKPLAISEF GGIDNSKPDD SYDDISSVRS
310 320 330 340 350
VSSFNHFLFN LMERQDNLFI SIPFVSDKAE WHITAANNYT SYSAALFIPD
360 370 380 390 400
NPQNLKNTTW RLNDKKYFFE LWKNVKGERV DITSSNPDIQ VQAFKDGGRL
410 420 430 440 450
YIALDNLDDN PQTVYLNNKN SWKDVSNVTK RSLYVNYNAG IEYTEQNVPS
460 470 480 490 500
MPESISIVPN QTIVLVADVS SSAFTNSIIR NKYYSSEYLK PISAGSSLSF
510 520 530 540 550
PFTGIESGSG RASLRMSIGR PVSASKKPVV KINGTAVSVP DNWKGYGQSN
560 570 580 590 600
RNIFFGMIEV PFDIQLLKNG DNNVDITFSD GGGHVSSMIL QVEKYTVSTL
610 620 630 640 650
QNGTFSEGLS AWQPLGNYGT VCVQTDNAGN NVACISGHAG LMQRVDMESG
660 670 680 690 700
RTYRFSADVK TEGACKLKVM LQDMSTGTVY TEEFSSPGNY KAVSFDFNST
710 720
VKKVVCAIVC ERQNDAAWID NIVLLPQN
Length:728
Mass (Da):81,281
Last modified:October 14, 2008 - v1
Checksum:i192BD9484B946F0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABQC02000019 Genomic DNA. Translation: EDY95427.1.
RefSeqiWP_007560960.1. NZ_DS990130.1.

Genome annotation databases

EnsemblBacteriaiEDY95427; EDY95427; BACPLE_01693.
PATRICi30481056. VBIBacPle58056_1657.

Cross-referencesi

Web resourcesi

Protein Spotlight

A gut's tale - Issue 158 of March 2014

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABQC02000019 Genomic DNA. Translation: EDY95427.1.
RefSeqiWP_007560960.1. NZ_DS990130.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AW7X-ray1.33A30-598[»]
ProteinModelPortaliB5CY96.
SMRiB5CY96.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi484018.BACPLE_01693.

Protein family/group databases

CAZyiGH86. Glycoside Hydrolase Family 86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEDY95427; EDY95427; BACPLE_01693.
PATRICi30481056. VBIBacPle58056_1657.

Phylogenomic databases

eggNOGiENOG4108KBQ. Bacteria.
ENOG410XSB9. LUCA.
OrthoDBiPOG091H0A4S.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR003305. CenC_carb-bd.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02018. CBM_4_9. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPORA_BACPM
AccessioniPrimary (citable) accession number: B5CY96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: October 14, 2008
Last modified: November 2, 2016
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Gut bacteria supply the human body with energy from dietary polysaccharides through glycosidases that are absent in the human genome. Beta-porphyranases, which are active on sulfated polysaccharides from marine red algae of the genus Porphyra, are present in marine bacteria. They are absent from metagenome data of gut bacteria, except from the genome of the gut bacterium B.plebeius isolated from Japanese individuals. Seaweeds make an important contribution to the diet in Japan and Porphyra (nori) is the most important nutritional seaweed used to prepare sushi, suggesting that seaweeds with associated marine bacteria have been the route by which genes coding for beta-porphyranases have been transferred in human gut B.plebeius genome (PubMed:23150581).1 Publication

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.