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B5CY96

- PORA_BACPM

UniProt

B5CY96 - PORA_BACPM

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Protein
Beta-porphyranase A
Gene
BACPLE_01693
Organism
Bacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Cleaves the sulfated polysaccharide porphyran at the (1->4) linkages between beta-D-galactopyranose and alpha-L-galactopyranose-6-sulfate, forming mostly the disaccharide alpha-L-galactopyranose-6-sulfate-(1->3)-beta-D-galactose. Some longer oligosaccharides of even number of residues are also observed. Inactive on the non-sulfated agarose portion of the porphyran backbone.1 Publication

Catalytic activityi

Hydrolysis of beta-D-galactopyranose-(1->4)-alpha-L-galactopyranose-6-sulfate linkages in porphyran.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531Substrate
Binding sitei87 – 871Substrate
Binding sitei114 – 1141Substrate
Binding sitei156 – 1561Substrate
Binding sitei237 – 2371Substrate
Binding sitei324 – 3241Substrate
Binding sitei331 – 3311Substrate
Binding sitei342 – 3421Substrate

GO - Molecular functioni

  1. hydrolase activity, acting on glycosyl bonds Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-porphyranase A (EC:3.2.1.178)
Alternative name(s):
Glycosyl hydrolase 86 family protein A
Short name:
GH86A
Gene namesi
ORF Names:BACPLE_01693
OrganismiBacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
Taxonomic identifieri484018 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Chaini23 – 728706Beta-porphyranase A
PRO_0000422034Add
BLAST

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi30 – 389
Helixi46 – 483
Beta strandi50 – 534
Helixi60 – 6910
Beta strandi75 – 773
Helixi80 – 889
Beta strandi105 – 1128
Turni117 – 1193
Helixi126 – 13813
Beta strandi141 – 1488
Beta strandi150 – 1523
Helixi154 – 1563
Helixi166 – 18520
Turni188 – 1936
Beta strandi195 – 2028
Turni206 – 2116
Helixi212 – 2165
Helixi218 – 2258
Helixi226 – 2283
Beta strandi230 – 24011
Helixi252 – 26918
Beta strandi275 – 28410
Turni294 – 2996
Helixi300 – 31213
Helixi313 – 3175
Beta strandi318 – 3247
Helixi330 – 3323
Helixi335 – 3373
Beta strandi346 – 3505
Turni355 – 3573
Beta strandi360 – 3623
Helixi366 – 3727
Beta strandi377 – 38610
Beta strandi389 – 3968
Beta strandi399 – 4068
Beta strandi408 – 4103
Beta strandi412 – 4176
Turni419 – 4246
Beta strandi425 – 43612
Turni437 – 4393
Beta strandi440 – 45011
Beta strandi453 – 4575
Beta strandi461 – 4699
Beta strandi476 – 4849
Beta strandi489 – 4913
Beta strandi498 – 5058
Beta strandi509 – 52113
Beta strandi529 – 5324
Beta strandi535 – 5373
Beta strandi551 – 56212
Helixi564 – 5663
Beta strandi569 – 5779
Beta strandi583 – 59614

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AW7X-ray1.33A30-598[»]
ProteinModelPortaliB5CY96.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini599 – 701103CBM-cenC
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni77 – 782Substrate binding
Regioni151 – 1522Substrate binding

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR003305. CenC_carb-bd.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02018. CBM_4_9. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B5CY96-1 [UniParc]FASTAAdd to Basket

« Hide

MSYKYIFLLS AFTLGVPPGI YCQGRNEVVV DYNTRRFLSG VSELDRSKYF    50
NIHSTSDDDK DVGKFLADYQ VGLGRKFWGP YSYAYNKTHE VGKYPQMKPY 100
SGNISVKRYI ATEHPYVQHI QGGIDVQAAG AWSAEYYSNS ELVPEFFEPL 150
NEPFVHANDA GFTVQGQAMR ELMVDFYASI GKHIHNNPRL NGKMKVIGYA 200
AAYPAWEDGN FNYWNTRMKM FIDRAGAYMD GFSVHLYDGI NVTGTDTKRS 250
GSNSEAVLDM VEAYSYIKFG HVKPLAISEF GGIDNSKPDD SYDDISSVRS 300
VSSFNHFLFN LMERQDNLFI SIPFVSDKAE WHITAANNYT SYSAALFIPD 350
NPQNLKNTTW RLNDKKYFFE LWKNVKGERV DITSSNPDIQ VQAFKDGGRL 400
YIALDNLDDN PQTVYLNNKN SWKDVSNVTK RSLYVNYNAG IEYTEQNVPS 450
MPESISIVPN QTIVLVADVS SSAFTNSIIR NKYYSSEYLK PISAGSSLSF 500
PFTGIESGSG RASLRMSIGR PVSASKKPVV KINGTAVSVP DNWKGYGQSN 550
RNIFFGMIEV PFDIQLLKNG DNNVDITFSD GGGHVSSMIL QVEKYTVSTL 600
QNGTFSEGLS AWQPLGNYGT VCVQTDNAGN NVACISGHAG LMQRVDMESG 650
RTYRFSADVK TEGACKLKVM LQDMSTGTVY TEEFSSPGNY KAVSFDFNST 700
VKKVVCAIVC ERQNDAAWID NIVLLPQN 728
Length:728
Mass (Da):81,281
Last modified:October 14, 2008 - v1
Checksum:i192BD9484B946F0E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
ABQC02000019 Genomic DNA. Translation: EDY95427.1.

Genome annotation databases

EnsemblBacteriaiEDY95427; EDY95427; BACPLE_01693.
PATRICi30481056. VBIBacPle58056_1657.

Cross-referencesi

Web resourcesi

Protein Spotlight

A gut's tale - Issue 158 of March 2014

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
ABQC02000019 Genomic DNA. Translation: EDY95427.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4AW7 X-ray 1.33 A 30-598 [» ]
ProteinModelPortali B5CY96.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai EDY95427 ; EDY95427 ; BACPLE_01693 .
PATRICi 30481056. VBIBacPle58056_1657.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
InterProi IPR003305. CenC_carb-bd.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02018. CBM_4_9. 1 hit.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Draft genome sequence of Bacteroides plebeius (DSM 17135)."
    Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D., Gordon J.
    Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17135 / JCM 12973 / M2.
  2. "Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes."
    Hehemann J.H., Kelly A.G., Pudlo N.A., Martens E.C., Boraston A.B.
    Proc. Natl. Acad. Sci. U.S.A. 109:19786-19791(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.33 ANGSTROMS) OF 30-598 IN COMPLEX WITH PORPHYRAN-AGAROSE-OLIGOSACCHARIDE, FUNCTION, CATALYTIC ACTIVITY.
    Strain: DSM 17135 / JCM 12973 / M2.

Entry informationi

Entry nameiPORA_BACPM
AccessioniPrimary (citable) accession number: B5CY96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: October 14, 2008
Last modified: April 16, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Gut bacteria supply the human body with energy from dietary polysaccharides through glycosidases that are absent in the human genome. Beta-porphyranases, which are active on sulfated polysaccharides from marine red algae of the genus Porphyra, are present in marine bacteria. They are absent from metagenome data of gut bacteria, except from the genome of the gut bacterium B.plebeius isolated from Japanese individuals. Seaweeds make an important contribution to the diet in Japan and Porphyra (nori) is the most important nutritional seaweed used to prepare sushi, suggesting that seaweeds with associated marine bacteria have been the route by which genes coding for beta-porphyranases have been transferred in human gut B.plebeius genome (1 Publication).

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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