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B5CY96 (PORA_BACPM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-porphyranase A

EC=3.2.1.178
Alternative name(s):
Glycosyl hydrolase 86 family protein A
Short name=GH86A
Gene names
ORF Names:BACPLE_01693
OrganismBacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
Taxonomic identifier484018 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

Protein attributes

Sequence length728 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves the sulfated polysaccharide porphyran at the (1->4) linkages between beta-D-galactopyranose and alpha-L-galactopyranose-6-sulfate, forming mostly the disaccharide alpha-L-galactopyranose-6-sulfate-(1->3)-beta-D-galactose. Some longer oligosaccharides of even number of residues are also observed. Inactive on the non-sulfated agarose portion of the porphyran backbone. Ref.2

Catalytic activity

Hydrolysis of beta-D-galactopyranose-(1->4)-alpha-L-galactopyranose-6-sulfate linkages in porphyran. Ref.2

Miscellaneous

Gut bacteria supply the human body with energy from dietary polysaccharides through glycosidases that are absent in the human genome. Beta-porphyranases, which are active on sulfated polysaccharides from marine red algae of the genus Porphyra, are present in marine bacteria. They are absent from metagenome data of gut bacteria, except from the genome of the gut bacterium B.plebeius isolated from Japanese individuals. Seaweeds make an important contribution to the diet in Japan and Porphyra (nori) is the most important nutritional seaweed used to prepare sushi, suggesting that seaweeds with associated marine bacteria have been the route by which genes coding for beta-porphyranases have been transferred in human gut B.plebeius genome (Ref.2).

Sequence similarities

Belongs to the glycosyl hydrolase 86 family.

Contains 1 CBM-cenC (cenC-type cellulose-binding) domain.

Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   Technical term3D-structure
Gene Ontology (GO)
   Molecular_functionhydrolase activity, acting on glycosyl bonds

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 728706Beta-porphyranase A
PRO_0000422034

Regions

Domain599 – 701103CBM-cenC
Region77 – 782Substrate binding
Region151 – 1522Substrate binding

Sites

Binding site531Substrate
Binding site871Substrate
Binding site1141Substrate
Binding site1561Substrate
Binding site2371Substrate
Binding site3241Substrate
Binding site3311Substrate
Binding site3421Substrate

Secondary structure

................................................................................................... 728
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
B5CY96 [UniParc].

Last modified October 14, 2008. Version 1.
Checksum: 192BD9484B946F0E

FASTA72881,281
        10         20         30         40         50         60 
MSYKYIFLLS AFTLGVPPGI YCQGRNEVVV DYNTRRFLSG VSELDRSKYF NIHSTSDDDK 

        70         80         90        100        110        120 
DVGKFLADYQ VGLGRKFWGP YSYAYNKTHE VGKYPQMKPY SGNISVKRYI ATEHPYVQHI 

       130        140        150        160        170        180 
QGGIDVQAAG AWSAEYYSNS ELVPEFFEPL NEPFVHANDA GFTVQGQAMR ELMVDFYASI 

       190        200        210        220        230        240 
GKHIHNNPRL NGKMKVIGYA AAYPAWEDGN FNYWNTRMKM FIDRAGAYMD GFSVHLYDGI 

       250        260        270        280        290        300 
NVTGTDTKRS GSNSEAVLDM VEAYSYIKFG HVKPLAISEF GGIDNSKPDD SYDDISSVRS 

       310        320        330        340        350        360 
VSSFNHFLFN LMERQDNLFI SIPFVSDKAE WHITAANNYT SYSAALFIPD NPQNLKNTTW 

       370        380        390        400        410        420 
RLNDKKYFFE LWKNVKGERV DITSSNPDIQ VQAFKDGGRL YIALDNLDDN PQTVYLNNKN 

       430        440        450        460        470        480 
SWKDVSNVTK RSLYVNYNAG IEYTEQNVPS MPESISIVPN QTIVLVADVS SSAFTNSIIR 

       490        500        510        520        530        540 
NKYYSSEYLK PISAGSSLSF PFTGIESGSG RASLRMSIGR PVSASKKPVV KINGTAVSVP 

       550        560        570        580        590        600 
DNWKGYGQSN RNIFFGMIEV PFDIQLLKNG DNNVDITFSD GGGHVSSMIL QVEKYTVSTL 

       610        620        630        640        650        660 
QNGTFSEGLS AWQPLGNYGT VCVQTDNAGN NVACISGHAG LMQRVDMESG RTYRFSADVK 

       670        680        690        700        710        720 
TEGACKLKVM LQDMSTGTVY TEEFSSPGNY KAVSFDFNST VKKVVCAIVC ERQNDAAWID 


NIVLLPQN 

« Hide

References

« Hide 'large scale' references
[1]"Draft genome sequence of Bacteroides plebeius (DSM 17135)."
Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D., Gordon J.
Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17135 / JCM 12973 / M2.
[2]"Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes."
Hehemann J.H., Kelly A.G., Pudlo N.A., Martens E.C., Boraston A.B.
Proc. Natl. Acad. Sci. U.S.A. 109:19786-19791(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.33 ANGSTROMS) OF 30-598 IN COMPLEX WITH PORPHYRAN-AGAROSE-OLIGOSACCHARIDE, FUNCTION, CATALYTIC ACTIVITY.
Strain: DSM 17135 / JCM 12973 / M2.

Web resources

Protein Spotlight

A gut's tale - Issue 158 of March 2014

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
ABQC02000019 Genomic DNA. Translation: EDY95427.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4AW7X-ray1.33A30-598[»]
ProteinModelPortalB5CY96.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEDY95427; EDY95427; BACPLE_01693.
PATRIC30481056. VBIBacPle58056_1657.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
InterProIPR003305. CenC_carb-bd.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02018. CBM_4_9. 1 hit.
[Graphical view]
SUPFAMSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry namePORA_BACPM
AccessionPrimary (citable) accession number: B5CY96
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: October 14, 2008
Last modified: April 16, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries