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B5CY96

- PORA_BACPM

UniProt

B5CY96 - PORA_BACPM

Protein

Beta-porphyranase A

Gene

BACPLE_01693

Organism
Bacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 21 (01 Oct 2014)
      Sequence version 1 (14 Oct 2008)
      Previous versions | rss
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    Functioni

    Cleaves the sulfated polysaccharide porphyran at the (1->4) linkages between beta-D-galactopyranose and alpha-L-galactopyranose-6-sulfate, forming mostly the disaccharide alpha-L-galactopyranose-6-sulfate-(1->3)-beta-D-galactose. Some longer oligosaccharides of even number of residues are also observed. Inactive on the non-sulfated agarose portion of the porphyran backbone.1 Publication

    Catalytic activityi

    Hydrolysis of beta-D-galactopyranose-(1->4)-alpha-L-galactopyranose-6-sulfate linkages in porphyran.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531Substrate
    Binding sitei87 – 871Substrate
    Binding sitei114 – 1141Substrate
    Binding sitei156 – 1561Substrate
    Binding sitei237 – 2371Substrate
    Binding sitei324 – 3241Substrate
    Binding sitei331 – 3311Substrate
    Binding sitei342 – 3421Substrate

    GO - Molecular functioni

    1. hydrolase activity, acting on glycosyl bonds Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-porphyranase A (EC:3.2.1.178)
    Alternative name(s):
    Glycosyl hydrolase 86 family protein A
    Short name:
    GH86A
    Gene namesi
    ORF Names:BACPLE_01693
    OrganismiBacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
    Taxonomic identifieri484018 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 728706Beta-porphyranase APRO_0000422034Add
    BLAST

    Structurei

    Secondary structure

    1
    728
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi30 – 389
    Helixi46 – 483
    Beta strandi50 – 534
    Helixi60 – 6910
    Beta strandi75 – 773
    Helixi80 – 889
    Beta strandi105 – 1128
    Turni117 – 1193
    Helixi126 – 13813
    Beta strandi141 – 1488
    Beta strandi150 – 1523
    Helixi154 – 1563
    Helixi166 – 18520
    Turni188 – 1936
    Beta strandi195 – 2028
    Turni206 – 2116
    Helixi212 – 2165
    Helixi218 – 2258
    Helixi226 – 2283
    Beta strandi230 – 24011
    Helixi252 – 26918
    Beta strandi275 – 28410
    Turni294 – 2996
    Helixi300 – 31213
    Helixi313 – 3175
    Beta strandi318 – 3247
    Helixi330 – 3323
    Helixi335 – 3373
    Beta strandi346 – 3505
    Turni355 – 3573
    Beta strandi360 – 3623
    Helixi366 – 3727
    Beta strandi377 – 38610
    Beta strandi389 – 3968
    Beta strandi399 – 4068
    Beta strandi408 – 4103
    Beta strandi412 – 4176
    Turni419 – 4246
    Beta strandi425 – 43612
    Turni437 – 4393
    Beta strandi440 – 45011
    Beta strandi453 – 4575
    Beta strandi461 – 4699
    Beta strandi476 – 4849
    Beta strandi489 – 4913
    Beta strandi498 – 5058
    Beta strandi509 – 52113
    Beta strandi529 – 5324
    Beta strandi535 – 5373
    Beta strandi551 – 56212
    Helixi564 – 5663
    Beta strandi569 – 5779
    Beta strandi583 – 59614

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4AW7X-ray1.33A30-598[»]
    ProteinModelPortaliB5CY96.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini599 – 701103CBM-cenCAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni77 – 782Substrate binding
    Regioni151 – 1522Substrate binding

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 86 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    InterProiIPR003305. CenC_carb-bd.
    IPR008979. Galactose-bd-like.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02018. CBM_4_9. 1 hit.
    [Graphical view]
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B5CY96-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYKYIFLLS AFTLGVPPGI YCQGRNEVVV DYNTRRFLSG VSELDRSKYF    50
    NIHSTSDDDK DVGKFLADYQ VGLGRKFWGP YSYAYNKTHE VGKYPQMKPY 100
    SGNISVKRYI ATEHPYVQHI QGGIDVQAAG AWSAEYYSNS ELVPEFFEPL 150
    NEPFVHANDA GFTVQGQAMR ELMVDFYASI GKHIHNNPRL NGKMKVIGYA 200
    AAYPAWEDGN FNYWNTRMKM FIDRAGAYMD GFSVHLYDGI NVTGTDTKRS 250
    GSNSEAVLDM VEAYSYIKFG HVKPLAISEF GGIDNSKPDD SYDDISSVRS 300
    VSSFNHFLFN LMERQDNLFI SIPFVSDKAE WHITAANNYT SYSAALFIPD 350
    NPQNLKNTTW RLNDKKYFFE LWKNVKGERV DITSSNPDIQ VQAFKDGGRL 400
    YIALDNLDDN PQTVYLNNKN SWKDVSNVTK RSLYVNYNAG IEYTEQNVPS 450
    MPESISIVPN QTIVLVADVS SSAFTNSIIR NKYYSSEYLK PISAGSSLSF 500
    PFTGIESGSG RASLRMSIGR PVSASKKPVV KINGTAVSVP DNWKGYGQSN 550
    RNIFFGMIEV PFDIQLLKNG DNNVDITFSD GGGHVSSMIL QVEKYTVSTL 600
    QNGTFSEGLS AWQPLGNYGT VCVQTDNAGN NVACISGHAG LMQRVDMESG 650
    RTYRFSADVK TEGACKLKVM LQDMSTGTVY TEEFSSPGNY KAVSFDFNST 700
    VKKVVCAIVC ERQNDAAWID NIVLLPQN 728
    Length:728
    Mass (Da):81,281
    Last modified:October 14, 2008 - v1
    Checksum:i192BD9484B946F0E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    ABQC02000019 Genomic DNA. Translation: EDY95427.1.

    Genome annotation databases

    EnsemblBacteriaiEDY95427; EDY95427; BACPLE_01693.
    PATRICi30481056. VBIBacPle58056_1657.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    A gut's tale - Issue 158 of March 2014

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    ABQC02000019 Genomic DNA. Translation: EDY95427.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4AW7 X-ray 1.33 A 30-598 [» ]
    ProteinModelPortali B5CY96.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai EDY95427 ; EDY95427 ; BACPLE_01693 .
    PATRICi 30481056. VBIBacPle58056_1657.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    InterProi IPR003305. CenC_carb-bd.
    IPR008979. Galactose-bd-like.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02018. CBM_4_9. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Draft genome sequence of Bacteroides plebeius (DSM 17135)."
      Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D., Gordon J.
      Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 17135 / JCM 12973 / M2.
    2. "Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes."
      Hehemann J.H., Kelly A.G., Pudlo N.A., Martens E.C., Boraston A.B.
      Proc. Natl. Acad. Sci. U.S.A. 109:19786-19791(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.33 ANGSTROMS) OF 30-598 IN COMPLEX WITH PORPHYRAN-AGAROSE-OLIGOSACCHARIDE, FUNCTION, CATALYTIC ACTIVITY.
      Strain: DSM 17135 / JCM 12973 / M2.

    Entry informationi

    Entry nameiPORA_BACPM
    AccessioniPrimary (citable) accession number: B5CY96
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2013
    Last sequence update: October 14, 2008
    Last modified: October 1, 2014
    This is version 21 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Gut bacteria supply the human body with energy from dietary polysaccharides through glycosidases that are absent in the human genome. Beta-porphyranases, which are active on sulfated polysaccharides from marine red algae of the genus Porphyra, are present in marine bacteria. They are absent from metagenome data of gut bacteria, except from the genome of the gut bacterium B.plebeius isolated from Japanese individuals. Seaweeds make an important contribution to the diet in Japan and Porphyra (nori) is the most important nutritional seaweed used to prepare sushi, suggesting that seaweeds with associated marine bacteria have been the route by which genes coding for beta-porphyranases have been transferred in human gut B.plebeius genome (PubMed:23150581).1 Publication

    Keywords - Technical termi

    3D-structure

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3