Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-porphyranase B

Gene

BACPLE_01689

Organism
Bacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves the sulfated polysaccharide porphyran at the (1->4) linkages between beta-D-galactopyranose and alpha-L-galactopyranose-6-sulfate, forming mostly the disaccharide alpha-L-galactopyranose-6-sulfate-(1->3)-beta-D-galactose. Some longer oligosaccharides of even number of residues are also observed. Inactive on the non-sulfated agarose portion of the porphyran backbone.1 Publication

Catalytic activityi

Hydrolysis of beta-D-galactopyranose-(1->4)-alpha-L-galactopyranose-6-sulfate linkages in porphyran.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72SubstrateBy similarity1
Binding sitei76SubstrateBy similarity1
Active sitei173NucleophileBy similarity1
Binding sitei173SubstrateBy similarity1
Active sitei178Proton donorBy similarity1
Binding sitei178SubstrateBy similarity1
Binding sitei284SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-porphyranase B (EC:3.2.1.178)
Alternative name(s):
Glycosyl hydrolase 86 family protein B
Short name:
GH16B
Gene namesi
ORF Names:BACPLE_01689
OrganismiBacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
Taxonomic identifieri484018 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000003452 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000042202521 – 321Beta-porphyranase BAdd BLAST301

Structurei

Secondary structure

1321
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi27 – 31Combined sources5
Helixi32 – 35Combined sources4
Beta strandi43 – 47Combined sources5
Helixi49 – 51Combined sources3
Beta strandi57 – 59Combined sources3
Turni62 – 64Combined sources3
Beta strandi65 – 68Combined sources4
Beta strandi70 – 72Combined sources3
Beta strandi79 – 81Combined sources3
Helixi83 – 85Combined sources3
Beta strandi86 – 89Combined sources4
Beta strandi92 – 96Combined sources5
Beta strandi98 – 104Combined sources7
Helixi106 – 108Combined sources3
Beta strandi110 – 118Combined sources9
Beta strandi120 – 124Combined sources5
Beta strandi130 – 138Combined sources9
Beta strandi141 – 144Combined sources4
Beta strandi146 – 151Combined sources6
Beta strandi155 – 163Combined sources9
Beta strandi165 – 179Combined sources15
Turni192 – 195Combined sources4
Helixi196 – 198Combined sources3
Beta strandi201 – 210Combined sources10
Beta strandi216 – 218Combined sources3
Beta strandi222 – 224Combined sources3
Turni233 – 235Combined sources3
Beta strandi238 – 246Combined sources9
Beta strandi249 – 254Combined sources6
Beta strandi257 – 262Combined sources6
Beta strandi277 – 282Combined sources6
Turni295 – 298Combined sources4
Turni301 – 304Combined sources4
Beta strandi305 – 318Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AWDX-ray2.40A/B21-321[»]
ProteinModelPortaliB5CY92.
SMRiB5CY92.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 319GH16PROSITE-ProRule annotationAdd BLAST289

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated
Contains 1 GH16 (glycosyl hydrolase family 16) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108ZIS. Bacteria.
ENOG4111NXQ. LUCA.
KOiK20830.
OrthoDBiPOG091H03M1.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B5CY92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKTVLYLSA ASLFLSSYTL KNDKEYSLAE EHIKNLPEAP EGYKWVVNED
60 70 80 90 100
YTDEFNGKRL NAAKWHAKSP YWTNGRPPAT FKAENVSVKK GCLRIINTVL
110 120 130 140 150
SPTEGLDGKP GDKYRLAGGA VASVKNQAHY GYYETRMKAS LTTMSSTFWL
160 170 180 190 200
SNRPVMKEIM KGGKKIKTWS SQELDIIETM GIIRSVNPDN PWNKTWNMQM
210 220 230 240 250
NSNTHYWYQE QGGKRTDNTA KRSDVVSYMT DPSAEDFHTY GCWWVDANTV
260 270 280 290 300
KFYYDGKYMY TIKPTTKYTD TPFDRPMFIH IVTETYDWEK QVPTAEDLKD
310 320
KDKSTTYYDW VRAYKLVPIE E
Length:321
Mass (Da):37,253
Last modified:October 14, 2008 - v1
Checksum:i56EAC9B6C773F4FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABQC02000019 Genomic DNA. Translation: EDY95423.1.
RefSeqiWP_007560951.1. NZ_DS990130.1.

Genome annotation databases

EnsemblBacteriaiEDY95423; EDY95423; BACPLE_01689.
KEGGiag:EDY95423.
PATRICi30481052. VBIBacPle58056_1655.

Cross-referencesi

Web resourcesi

Protein Spotlight

A gut's tale - Issue 158 of March 2014

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABQC02000019 Genomic DNA. Translation: EDY95423.1.
RefSeqiWP_007560951.1. NZ_DS990130.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AWDX-ray2.40A/B21-321[»]
ProteinModelPortaliB5CY92.
SMRiB5CY92.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEDY95423; EDY95423; BACPLE_01689.
KEGGiag:EDY95423.
PATRICi30481052. VBIBacPle58056_1655.

Phylogenomic databases

eggNOGiENOG4108ZIS. Bacteria.
ENOG4111NXQ. LUCA.
KOiK20830.
OrthoDBiPOG091H03M1.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPORB_BACPM
AccessioniPrimary (citable) accession number: B5CY92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: October 14, 2008
Last modified: November 30, 2016
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Gut bacteria supply the human body with energy from dietary polysaccharides through glycosidases that are absent in the human genome. Beta-porphyranases, which are active on sulfated polysaccharides from marine red algae of the genus Porphyra, are present in marine bacteria. They are absent from metagenome data of gut bacteria, except from the genome of the gut bacterium B.plebeius isolated from Japanese individuals. Seaweeds make an important contribution to the diet in Japan and Porphyra (nori) is the most important nutritional seaweed used to prepare sushi, suggesting that seaweeds with associated marine bacteria have been the route by which genes coding for beta-porphyranases have been transferred in human gut B.plebeius genome (PubMed:20376150 and PubMed:23150581).

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.