Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

B5CY92

- PORB_BACPM

UniProt

B5CY92 - PORB_BACPM

Protein

Beta-porphyranase B

Gene

BACPLE_01689

Organism
Bacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 19 (01 Oct 2014)
      Sequence version 1 (14 Oct 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Cleaves the sulfated polysaccharide porphyran at the (1->4) linkages between beta-D-galactopyranose and alpha-L-galactopyranose-6-sulfate, forming mostly the disaccharide alpha-L-galactopyranose-6-sulfate-(1->3)-beta-D-galactose. Some longer oligosaccharides of even number of residues are also observed. Inactive on the non-sulfated agarose portion of the porphyran backbone.1 Publication

    Catalytic activityi

    Hydrolysis of beta-D-galactopyranose-(1->4)-alpha-L-galactopyranose-6-sulfate linkages in porphyran.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei72 – 721SubstrateBy similarity
    Binding sitei76 – 761SubstrateBy similarity
    Active sitei173 – 1731NucleophileBy similarity
    Binding sitei173 – 1731SubstrateBy similarity
    Active sitei178 – 1781Proton donorBy similarity
    Binding sitei178 – 1781SubstrateBy similarity
    Binding sitei284 – 2841SubstrateBy similarity

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-porphyranase B (EC:3.2.1.178)
    Alternative name(s):
    Glycosyl hydrolase 86 family protein B
    Short name:
    GH16B
    Gene namesi
    ORF Names:BACPLE_01689
    OrganismiBacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
    Taxonomic identifieri484018 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 321301Beta-porphyranase BPRO_0000422025Add
    BLAST

    Structurei

    Secondary structure

    1
    321
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi27 – 315
    Helixi32 – 354
    Beta strandi43 – 475
    Helixi49 – 513
    Beta strandi57 – 593
    Turni62 – 643
    Beta strandi65 – 684
    Beta strandi70 – 723
    Beta strandi79 – 813
    Helixi83 – 853
    Beta strandi86 – 894
    Beta strandi92 – 965
    Beta strandi98 – 1047
    Helixi106 – 1083
    Beta strandi110 – 1189
    Beta strandi120 – 1245
    Beta strandi130 – 1389
    Beta strandi141 – 1444
    Beta strandi146 – 1516
    Beta strandi155 – 1639
    Beta strandi165 – 17915
    Turni192 – 1954
    Helixi196 – 1983
    Beta strandi201 – 21010
    Beta strandi216 – 2183
    Beta strandi222 – 2243
    Turni233 – 2353
    Beta strandi238 – 2469
    Beta strandi249 – 2546
    Beta strandi257 – 2626
    Beta strandi277 – 2826
    Turni295 – 2984
    Turni301 – 3044
    Beta strandi305 – 31814

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4AWDX-ray2.40A/B21-321[»]
    ProteinModelPortaliB5CY92.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B5CY92-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKTVLYLSA ASLFLSSYTL KNDKEYSLAE EHIKNLPEAP EGYKWVVNED    50
    YTDEFNGKRL NAAKWHAKSP YWTNGRPPAT FKAENVSVKK GCLRIINTVL 100
    SPTEGLDGKP GDKYRLAGGA VASVKNQAHY GYYETRMKAS LTTMSSTFWL 150
    SNRPVMKEIM KGGKKIKTWS SQELDIIETM GIIRSVNPDN PWNKTWNMQM 200
    NSNTHYWYQE QGGKRTDNTA KRSDVVSYMT DPSAEDFHTY GCWWVDANTV 250
    KFYYDGKYMY TIKPTTKYTD TPFDRPMFIH IVTETYDWEK QVPTAEDLKD 300
    KDKSTTYYDW VRAYKLVPIE E 321
    Length:321
    Mass (Da):37,253
    Last modified:October 14, 2008 - v1
    Checksum:i56EAC9B6C773F4FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    ABQC02000019 Genomic DNA. Translation: EDY95423.1.

    Genome annotation databases

    EnsemblBacteriaiEDY95423; EDY95423; BACPLE_01689.
    PATRICi30481052. VBIBacPle58056_1655.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    A gut's tale - Issue 158 of March 2014

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    ABQC02000019 Genomic DNA. Translation: EDY95423.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4AWD X-ray 2.40 A/B 21-321 [» ]
    ProteinModelPortali B5CY92.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai EDY95423 ; EDY95423 ; BACPLE_01689 .
    PATRICi 30481052. VBIBacPle58056_1655.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Draft genome sequence of Bacteroides plebeius (DSM 17135)."
      Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D., Gordon J.
      Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 17135 / JCM 12973 / M2.
    2. "Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota."
      Hehemann J.H., Correc G., Barbeyron T., Helbert W., Czjzek M., Michel G.
      Nature 464:908-912(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
      Strain: DSM 17135 / JCM 12973 / M2.
    3. "Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes."
      Hehemann J.H., Kelly A.G., Pudlo N.A., Martens E.C., Boraston A.B.
      Proc. Natl. Acad. Sci. U.S.A. 109:19786-19791(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 21-321, FUNCTION, CATALYTIC ACTIVITY.
      Strain: DSM 17135 / JCM 12973 / M2.

    Entry informationi

    Entry nameiPORB_BACPM
    AccessioniPrimary (citable) accession number: B5CY92
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2013
    Last sequence update: October 14, 2008
    Last modified: October 1, 2014
    This is version 19 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Gut bacteria supply the human body with energy from dietary polysaccharides through glycosidases that are absent in the human genome. Beta-porphyranases, which are active on sulfated polysaccharides from marine red algae of the genus Porphyra, are present in marine bacteria. They are absent from metagenome data of gut bacteria, except from the genome of the gut bacterium B.plebeius isolated from Japanese individuals. Seaweeds make an important contribution to the diet in Japan and Porphyra (nori) is the most important nutritional seaweed used to prepare sushi, suggesting that seaweeds with associated marine bacteria have been the route by which genes coding for beta-porphyranases have been transferred in human gut B.plebeius genome (PubMed:20376150 and PubMed:23150581).

    Keywords - Technical termi

    3D-structure

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3