SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B5CY73

- BAGA_BACPM

UniProt

B5CY73 - BAGA_BACPM

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Beta-agarase

Gene
BACPLE_01670
Organism
Bacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Cleaves the beta-1,4-linkages between beta-D-galactose and alpha-L-3,6-anhydro-galactose residues in agarose. Cleaves agarose in a random manner with retention of the anomeric-bond configuration, producing beta-anomers that give rise progressively to alpha-anomers when mutarotation takes place.1 Publication

Catalytic activityi

Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei164 – 1641Substrate By similarity
Active sitei167 – 1671Nucleophile By similarity
Active sitei172 – 1721Proton donor By similarity
Binding sitei197 – 1971Substrate By similarity

GO - Molecular functioni

  1. beta-agarase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-agarase (EC:3.2.1.81)
Alternative name(s):
Glycosyl hydrolase 16 family protein A
Short name:
GH16A
Gene namesi
ORF Names:BACPLE_01670
OrganismiBacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
Taxonomic identifieri484018 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Reviewed predictionAdd
BLAST
Chaini19 – 316298Beta-agarasePRO_0000422029Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB5CY73.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 9711Substrate binding By similarityAdd
BLAST
Regioni102 – 1043Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B5CY73-1 [UniParc]FASTAAdd to Basket

« Hide

MKRKLFTICL ASLQFACAAE NLNNKSYEWD IYPVPANAGD GMVWKLHPQS    50
DDFNYIADEK DKGKEFYAKW TDFYHNHWTG PAPTIWQRDH VSVSDGFLKI 100
RASRPEDVPL KKVVSGPNTK ELPGTYTGCI TSKTRVKYPV YVEAYAKLSN 150
STMASDVWML SPDDTQEIDI IEAYGGDRDG GGYGADRLHL SHHIFIRQPF 200
KDYQPKDSGS WYKDDKGTLW RDDFHRVGVF WKDPFTLEYY VDGELVRTIS 250
GKDIIDPNNY TGGTGLVKDM DIIINMEDQS WRAVKGLSPT DEELKNVEDH 300
TFLVDWIRVY TLVPEE 316
Length:316
Mass (Da):36,274
Last modified:October 14, 2008 - v1
Checksum:i390E5D966B4018F2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
ABQC02000019 Genomic DNA. Translation: EDY95404.1.

Genome annotation databases

EnsemblBacteriaiEDY95404; EDY95404; BACPLE_01670.
PATRICi30481018. VBIBacPle58056_1638.

Cross-referencesi

Web resourcesi

Protein Spotlight

A gut's tale - Issue 158 of March 2014

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
ABQC02000019 Genomic DNA. Translation: EDY95404.1 .

3D structure databases

ProteinModelPortali B5CY73.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai EDY95404 ; EDY95404 ; BACPLE_01670 .
PATRICi 30481018. VBIBacPle58056_1638.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Draft genome sequence of Bacteroides plebeius (DSM 17135)."
    Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D., Gordon J.
    Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17135 / JCM 12973 / M2.
  2. "Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota."
    Hehemann J.H., Correc G., Barbeyron T., Helbert W., Czjzek M., Michel G.
    Nature 464:908-912(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
    Strain: DSM 17135 / JCM 12973 / M2.
  3. "Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes."
    Hehemann J.H., Kelly A.G., Pudlo N.A., Martens E.C., Boraston A.B.
    Proc. Natl. Acad. Sci. U.S.A. 109:19786-19791(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
    Strain: DSM 17135 / JCM 12973 / M2.

Entry informationi

Entry nameiBAGA_BACPM
AccessioniPrimary (citable) accession number: B5CY73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: October 14, 2008
Last modified: April 16, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Gut bacteria supply the human body with energy from dietary polysaccharides through glycosidases that are absent in the human genome. Beta-agarases, which are active on agarose from marine red algae of the genus Porphyra, are present in marine bacteria. They are absent from metagenome data of gut bacteria, except from the genome of the gut bacterium B.plebeius isolated from Japanese individuals. Seaweeds make an important contribution to the diet in Japan and Porphyra (nori) is the most important nutritional seaweed used to prepare sushi, suggesting that seaweeds with associated marine bacteria have been the route by which genes coding for beta-agarases have been transferred in human gut B.plebeius genome (1 Publication and 1 Publication).

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi