Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B5CY73 (BAGA_BACPM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-agarase

EC=3.2.1.81
Alternative name(s):
Glycosyl hydrolase 16 family protein A
Short name=GH16A
Gene names
ORF Names:BACPLE_01670
OrganismBacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
Taxonomic identifier484018 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves the beta-1,4-linkages between beta-D-galactose and alpha-L-3,6-anhydro-galactose residues in agarose. Cleaves agarose in a random manner with retention of the anomeric-bond configuration, producing beta-anomers that give rise progressively to alpha-anomers when mutarotation takes place. Ref.3

Catalytic activity

Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product. Ref.3

Miscellaneous

Gut bacteria supply the human body with energy from dietary polysaccharides through glycosidases that are absent in the human genome. Beta-agarases, which are active on agarose from marine red algae of the genus Porphyra, are present in marine bacteria. They are absent from metagenome data of gut bacteria, except from the genome of the gut bacterium B.plebeius isolated from Japanese individuals. Seaweeds make an important contribution to the diet in Japan and Porphyra (nori) is the most important nutritional seaweed used to prepare sushi, suggesting that seaweeds with associated marine bacteria have been the route by which genes coding for beta-agarases have been transferred in human gut B.plebeius genome (Ref.2 and Ref.3).

Sequence similarities

Belongs to the glycosyl hydrolase 16 family.

Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-agarase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 316298Beta-agarase
PRO_0000422029

Regions

Region87 – 9711Substrate binding By similarity
Region102 – 1043Substrate binding By similarity

Sites

Active site1671Nucleophile By similarity
Active site1721Proton donor By similarity
Binding site1641Substrate By similarity
Binding site1971Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B5CY73 [UniParc].

Last modified October 14, 2008. Version 1.
Checksum: 390E5D966B4018F2

FASTA31636,274
        10         20         30         40         50         60 
MKRKLFTICL ASLQFACAAE NLNNKSYEWD IYPVPANAGD GMVWKLHPQS DDFNYIADEK 

        70         80         90        100        110        120 
DKGKEFYAKW TDFYHNHWTG PAPTIWQRDH VSVSDGFLKI RASRPEDVPL KKVVSGPNTK 

       130        140        150        160        170        180 
ELPGTYTGCI TSKTRVKYPV YVEAYAKLSN STMASDVWML SPDDTQEIDI IEAYGGDRDG 

       190        200        210        220        230        240 
GGYGADRLHL SHHIFIRQPF KDYQPKDSGS WYKDDKGTLW RDDFHRVGVF WKDPFTLEYY 

       250        260        270        280        290        300 
VDGELVRTIS GKDIIDPNNY TGGTGLVKDM DIIINMEDQS WRAVKGLSPT DEELKNVEDH 

       310 
TFLVDWIRVY TLVPEE 

« Hide

References

« Hide 'large scale' references
[1]"Draft genome sequence of Bacteroides plebeius (DSM 17135)."
Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D., Gordon J.
Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17135 / JCM 12973 / M2.
[2]"Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota."
Hehemann J.H., Correc G., Barbeyron T., Helbert W., Czjzek M., Michel G.
Nature 464:908-912(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
Strain: DSM 17135 / JCM 12973 / M2.
[3]"Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes."
Hehemann J.H., Kelly A.G., Pudlo N.A., Martens E.C., Boraston A.B.
Proc. Natl. Acad. Sci. U.S.A. 109:19786-19791(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY.
Strain: DSM 17135 / JCM 12973 / M2.

Web resources

Protein Spotlight

A gut's tale - Issue 158 of March 2014

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
ABQC02000019 Genomic DNA. Translation: EDY95404.1.

3D structure databases

ProteinModelPortalB5CY73.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEDY95404; EDY95404; BACPLE_01670.
PATRIC30481018. VBIBacPle58056_1638.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBAGA_BACPM
AccessionPrimary (citable) accession number: B5CY73
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: October 14, 2008
Last modified: April 16, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries