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B5CY73

- BAGA_BACPM

UniProt

B5CY73 - BAGA_BACPM

Protein

Beta-agarase

Gene

BACPLE_01670

Organism
Bacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 18 (01 Oct 2014)
      Sequence version 1 (14 Oct 2008)
      Previous versions | rss
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    Functioni

    Cleaves the beta-1,4-linkages between beta-D-galactose and alpha-L-3,6-anhydro-galactose residues in agarose. Cleaves agarose in a random manner with retention of the anomeric-bond configuration, producing beta-anomers that give rise progressively to alpha-anomers when mutarotation takes place.1 Publication

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei164 – 1641SubstrateBy similarity
    Active sitei167 – 1671NucleophileBy similarity
    Active sitei172 – 1721Proton donorBy similarity
    Binding sitei197 – 1971SubstrateBy similarity

    GO - Molecular functioni

    1. beta-agarase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-agarase (EC:3.2.1.81)
    Alternative name(s):
    Glycosyl hydrolase 16 family protein A
    Short name:
    GH16A
    Gene namesi
    ORF Names:BACPLE_01670
    OrganismiBacteroides plebeius (strain DSM 17135 / JCM 12973 / M2)
    Taxonomic identifieri484018 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 316298Beta-agarasePRO_0000422029Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliB5CY73.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni87 – 9711Substrate bindingBy similarityAdd
    BLAST
    Regioni102 – 1043Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B5CY73-1 [UniParc]FASTAAdd to Basket

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    MKRKLFTICL ASLQFACAAE NLNNKSYEWD IYPVPANAGD GMVWKLHPQS    50
    DDFNYIADEK DKGKEFYAKW TDFYHNHWTG PAPTIWQRDH VSVSDGFLKI 100
    RASRPEDVPL KKVVSGPNTK ELPGTYTGCI TSKTRVKYPV YVEAYAKLSN 150
    STMASDVWML SPDDTQEIDI IEAYGGDRDG GGYGADRLHL SHHIFIRQPF 200
    KDYQPKDSGS WYKDDKGTLW RDDFHRVGVF WKDPFTLEYY VDGELVRTIS 250
    GKDIIDPNNY TGGTGLVKDM DIIINMEDQS WRAVKGLSPT DEELKNVEDH 300
    TFLVDWIRVY TLVPEE 316
    Length:316
    Mass (Da):36,274
    Last modified:October 14, 2008 - v1
    Checksum:i390E5D966B4018F2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    ABQC02000019 Genomic DNA. Translation: EDY95404.1.

    Genome annotation databases

    EnsemblBacteriaiEDY95404; EDY95404; BACPLE_01670.
    PATRICi30481018. VBIBacPle58056_1638.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    A gut's tale - Issue 158 of March 2014

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    ABQC02000019 Genomic DNA. Translation: EDY95404.1 .

    3D structure databases

    ProteinModelPortali B5CY73.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai EDY95404 ; EDY95404 ; BACPLE_01670 .
    PATRICi 30481018. VBIBacPle58056_1638.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Draft genome sequence of Bacteroides plebeius (DSM 17135)."
      Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D., Gordon J.
      Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 17135 / JCM 12973 / M2.
    2. "Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota."
      Hehemann J.H., Correc G., Barbeyron T., Helbert W., Czjzek M., Michel G.
      Nature 464:908-912(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
      Strain: DSM 17135 / JCM 12973 / M2.
    3. "Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes."
      Hehemann J.H., Kelly A.G., Pudlo N.A., Martens E.C., Boraston A.B.
      Proc. Natl. Acad. Sci. U.S.A. 109:19786-19791(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.
      Strain: DSM 17135 / JCM 12973 / M2.

    Entry informationi

    Entry nameiBAGA_BACPM
    AccessioniPrimary (citable) accession number: B5CY73
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2013
    Last sequence update: October 14, 2008
    Last modified: October 1, 2014
    This is version 18 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Gut bacteria supply the human body with energy from dietary polysaccharides through glycosidases that are absent in the human genome. Beta-agarases, which are active on agarose from marine red algae of the genus Porphyra, are present in marine bacteria. They are absent from metagenome data of gut bacteria, except from the genome of the gut bacterium B.plebeius isolated from Japanese individuals. Seaweeds make an important contribution to the diet in Japan and Porphyra (nori) is the most important nutritional seaweed used to prepare sushi, suggesting that seaweeds with associated marine bacteria have been the route by which genes coding for beta-agarases have been transferred in human gut B.plebeius genome (PubMed:20376150 and PubMed:23150581).

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3