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Protein

TATA-binding protein-associated factor BTAF1

Gene

BTAF1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in meristem development. Acts as positive regulator of the CUC-STM pathway in shoot apical meristem (SAM) neo-formation.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1476 – 14838ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • positive regulation of shoot apical meristem development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-2087-MONOMER.
ARA:GQT-2726-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
TATA-binding protein-associated factor BTAF1 (EC:3.6.4.-)
Short name:
AtBTAF1
Alternative name(s):
Protein BTAF1 homolog
Protein ROOT GROWTH DEFECTIVE 3
Gene namesi
Name:BTAF1
Synonyms:RGD3
Ordered Locus Names:At3g54280
ORF Names:F24B22.240
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G54280.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Impaired in root growth and true leaf development.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi932 – 9321G → E in rgd3-1; impaired in adventitious root formation at the restrictive temperature of 28 degrees Celsius. Impaired in shoot regeneration. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20452045TATA-binding protein-associated factor BTAF1PRO_0000423018Add
BLAST

Proteomic databases

PaxDbiB5BT18.
PRIDEiB5BT18.

PTM databases

iPTMnetiB5BT18.

Expressioni

Gene expression databases

ExpressionAtlasiB5BT18. baseline and differential.
GenevisibleiB5BT18. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G54280.2.

Structurei

3D structure databases

ProteinModelPortaliB5BT18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati3 – 4038HEAT 1Add
BLAST
Repeati41 – 7838HEAT 2Add
BLAST
Repeati584 – 62138HEAT 3Add
BLAST
Repeati702 – 73938HEAT 4Add
BLAST
Repeati1288 – 132538HEAT 5Add
BLAST
Repeati1367 – 140539HEAT 6Add
BLAST
Domaini1463 – 1633171Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini1805 – 1976172Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1584 – 15874DEAH box

Sequence similaritiesi

Belongs to the helicase family.Curated
Contains 6 HEAT repeats.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0392. Eukaryota.
ENOG410XP4Z. LUCA.
HOGENOMiHOG000210415.
KOiK15192.
PhylomeDBiB5BT18.

Family and domain databases

Gene3Di1.25.10.10. 5 hits.
3.40.50.300. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR022707. DUF3535.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF12054. DUF3535. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: B5BT18-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQQQSSRLN RLLTLLDTGS TQATRLTAAK QIGDIAKSHP QDLSSLLRKV
60 70 80 90 100
LHHLRSKKWD TRVAAAHAIG AIVLNVKHPS LSELLNSLAT KLGEAGISDN
110 120 130 140 150
VDEVVAFRNL QSKILANAPF RSFEMNKVLE FGALLASGGQ EYDILNDNSK
160 170 180 190 200
NPRDRVARQK KNLRRRLGLD MCEQFMDVNE MIRDEDLIEQ KSNVPANGVG
210 220 230 240 250
NRLYANCSPH HIQQFVSRMV PRVNSRRPSA RELNLLKRKA KISSKDQAKG
260 270 280 290 300
SCEVADVEMS SSHVASTSKR ILSDSLDSSK ADIGNEDDIE PDGDGKWPFH
310 320 330 340 350
SFVEQLILDM FDPAWEIRHG SVMALREILM LHGGSAGVST EEFSSDNGFE
360 370 380 390 400
LKDVLNKVTR EREIDLNMQV SENELEPLRK RPKIEDPSKS FIDNTVLEVI
410 420 430 440 450
GGDYDINVKD EDAEFLLPPV KVNGQTDCSS TKLEPQSSMD DSTSHSEINH
460 470 480 490 500
VAEVNNHFED KSFIEEPVIP KQQEENLEVL DLVKQARHSW IKNFEFLQDC
510 520 530 540 550
TIRFLCVLSL DRFGDYISDQ VVAPVREACA QALGATFKYM NPSLIYETLN
560 570 580 590 600
ILLQMQRRPE WEIRHGSLLG IKYLVAVRQE MLQDLLGYIL PACKAGLEDS
610 620 630 640 650
DDDVRAVAAD ALIPAAAAIV SLRGQTLLSI VMLLWDILLE LDDLSPSTSS
660 670 680 690 700
IMNLLAEIYS QDDMTLVMHE ELSLGEEQNI ELNEMGHIES IGERRDVKES
710 720 730 740 750
PYALSGLAPR LWPFTRHDIT SVRFSAIRTL ERLLEAGCRK NISGQSKSSF
760 770 780 790 800
WPSSILGDTL RIVFQNLLLE STEEILECSE RVWRLLVQCP VDDLEDTAKF
810 820 830 840 850
YMASWIELAA TPYGSTLDAT KMFWPVAPPR KSHFKAAAKM KAVKLENEAS
860 870 880 890 900
SILGFDYARS SASLEKQEDA SARSTKIIVG SDMEMSVTRT RVVTASALGI
910 920 930 940 950
FASRLREGSM QFVVDPLSST LTSMSGVQRQ VGSIVLISWF RETKCKAPSD
960 970 980 990 1000
GSGSLPGFPS PLKKWLLDLL ACADPAFPTK DIFLPYAELS RTYTKMRNEA
1010 1020 1030 1040 1050
SQLLHTVETC HCFDKLLSTN KLNVESVTAD ETIDFASTLD LWNKESAGNE
1060 1070 1080 1090 1100
SLEKQVFEDV ESSRQQLLST AGYLKCVQSN LHITVTSLVA AAVVWMSEFP
1110 1120 1130 1140 1150
ARLNPIILPL MASIKREQEQ ILQQIAAEAL AELIAYCVDR KPSPNDKLIK
1160 1170 1180 1190 1200
NICSLTCMDP SETPQASIIS SMDIVDDMDF LSSRSNTGKQ KAKVVLASGE
1210 1220 1230 1240 1250
DRSKVEGFIT RRGSELALKH LSLKFGGSLF DKLPKLWECL TEVLVPEIPS
1260 1270 1280 1290 1300
DQQKIDLKIE SISDPQVLIN NIQVVRSIAP VMEETLKPRL LSLLPCIFKC
1310 1320 1330 1340 1350
VRHSHVAVRL AASRCVMTMA KSMTTDVMAA VVESAIPMLG DLTCISGRQG
1360 1370 1380 1390 1400
AGMLIGLLVQ GLGVELVPYS PLLVVPLLRC MSDVDSSVRQ SVTRSFAALV
1410 1420 1430 1440 1450
PMLPLARGVP PPVGLSKDLS SNAEDAKFLE QLLDNSHIDD YKLCTELKVQ
1460 1470 1480 1490 1500
LRRYQQEGIN WLGFLKRFKL HGILCDDMGL GKTLQASAIV ASDAAERRGS
1510 1520 1530 1540 1550
TDELDVFPSI IVCPSTLVGH WAFEIEKYID LSLLSVLQYV GSAQDRVSLR
1560 1570 1580 1590 1600
EQFNNHNVII TSYDVVRKDV DYLTQFSWNY CILDEGHIIK NAKSKITAAV
1610 1620 1630 1640 1650
KQLKAQHRLI LSGTPIQNNI MELWSLFDFL MPGFLGTERQ FQASYGKPLL
1660 1670 1680 1690 1700
AARDPKCSAK DAEAGVLAME ALHKQVMPFL LRRTKEEVLS DLPEKIIQDR
1710 1720 1730 1740 1750
YCDLSPVQLK LYEQFSGSSA KQEISSIIKV DGSADSGNAD VAPTKASTHV
1760 1770 1780 1790 1800
FQALQYLLKL CSHPLLVLGD KVTEPVASDL AAMINGCSDI ITELHKVQHS
1810 1820 1830 1840 1850
PKLVALQEIL EECGIGSDAS SSDGTLSVGQ HRVLIFAQHK ALLDIIEKDL
1860 1870 1880 1890 1900
FQAHMKSVTY MRLDGSVVPE KRFEIVKAFN SDPTIDVLLL TTHVGGLGLN
1910 1920 1930 1940 1950
LTSADTLVFM EHDWNPMRDH QAMDRAHRLG QKRVVNVHRL IMRGTLEEKV
1960 1970 1980 1990 2000
MSLQKFKVSV ANTVINAENA SMKTMNTDQL LDLFASAETS KKGGGSSKKG
2010 2020 2030 2040
SEDNDQIAGT GKGMKAILGN LEELWDQSQY TEEYNLSQFL TKLNG
Length:2,045
Mass (Da):227,055
Last modified:October 14, 2008 - v1
Checksum:iD3D8D42603F71DE6
GO

Sequence cautioni

The sequence CAB71002.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB440793 mRNA. Translation: BAG70033.1.
AL132957 Genomic DNA. Translation: CAB71002.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79207.1.
PIRiT47587.
RefSeqiNP_190996.3. NM_115288.7. [B5BT18-1]
UniGeneiAt.35125.

Genome annotation databases

EnsemblPlantsiAT3G54280.1; AT3G54280.1; AT3G54280. [B5BT18-1]
GeneIDi824595.
KEGGiath:AT3G54280.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB440793 mRNA. Translation: BAG70033.1.
AL132957 Genomic DNA. Translation: CAB71002.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79207.1.
PIRiT47587.
RefSeqiNP_190996.3. NM_115288.7. [B5BT18-1]
UniGeneiAt.35125.

3D structure databases

ProteinModelPortaliB5BT18.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G54280.2.

PTM databases

iPTMnetiB5BT18.

Proteomic databases

PaxDbiB5BT18.
PRIDEiB5BT18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G54280.1; AT3G54280.1; AT3G54280. [B5BT18-1]
GeneIDi824595.
KEGGiath:AT3G54280.

Organism-specific databases

TAIRiAT3G54280.

Phylogenomic databases

eggNOGiKOG0392. Eukaryota.
ENOG410XP4Z. LUCA.
HOGENOMiHOG000210415.
KOiK15192.
PhylomeDBiB5BT18.

Enzyme and pathway databases

BioCyciARA:GQT-2087-MONOMER.
ARA:GQT-2726-MONOMER.

Miscellaneous databases

PROiB5BT18.

Gene expression databases

ExpressionAtlasiB5BT18. baseline and differential.
GenevisibleiB5BT18. AT.

Family and domain databases

Gene3Di1.25.10.10. 5 hits.
3.40.50.300. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR022707. DUF3535.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF12054. DUF3535. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of novel meristem factors involved in shoot regeneration through the analysis of temperature-sensitive mutants of Arabidopsis."
    Tamaki H., Konishi M., Daimon Y., Aida M., Tasaka M., Sugiyama M.
    Plant J. 57:1027-1039(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-932.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Genetic analysis of adventitious root formation with a novel series of temperature-sensitive mutants of Arabidopsis thaliana."
    Konishi M., Sugiyama M.
    Development 130:5637-5647(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTANT RGD3-1.
  5. "Genome-wide comparative in silico analysis of the RNA helicase gene family in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza sativa."
    Xu R., Zhang S., Huang J., Zheng C.
    PLoS ONE 8:E78982-E78982(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.

Entry informationi

Entry nameiBTAF1_ARATH
AccessioniPrimary (citable) accession number: B5BT18
Secondary accession number(s): Q9M378
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: October 14, 2008
Last modified: June 8, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.