Skip Header

Contribute Send feedback
Read comments (?) or add your own

B5BLL3 (ISPH_SALPK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2
Gene names
Name:ispH
Ordered Locus Names:SSPA0046
OrganismSalmonella paratyphi A (strain AKU_12601) [Complete proteome] [HAMAP]
Taxonomic identifier554290 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella ›

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP-Rule MF_00191

Catalytic activity

Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP-Rule MF_00191

Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP-Rule MF_00191

Cofactor

Binds 1 3Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_00191

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP-Rule MF_00191

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP-Rule MF_00191

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00191

Sequence similarities

Belongs to the IspH family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3163164-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP-Rule MF_00191
PRO_1000098975

Regions

Region225 – 2273Substrate binding By similarity

Sites

Metal binding121Iron-sulfur (3Fe-4S) By similarity
Metal binding961Iron-sulfur (3Fe-4S) By similarity
Metal binding1971Iron-sulfur (3Fe-4S) By similarity
Binding site411Substrate By similarity
Binding site741Substrate By similarity
Binding site1241Substrate By similarity
Binding site1671Substrate By similarity
Binding site2691Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B5BLL3 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 9084F204D6F197A0

FASTA31634,497
        10         20         30         40         50         60 
MQILLANPRG FCAGVDRAIS IVENALAIYG APIYVRHEVV HNRYVVDSLR KRGAIFIEQI 

        70         80         90        100        110        120 
SEVPDGAILI FSAHGVSQAV RNEAKSRDLT VFDATCPLVT KVHMEVARAS RRGEESILIG 

       130        140        150        160        170        180 
HAGHPEVEGT MGQYSNPEGG MFLVESPEDV WTLNVKNEGK LSFMTQTTLS VDDTSDVIDA 

       190        200        210        220        230        240 
LRKRFPKIVG PRKDDICYAT TNRQEAVRAL AEQADVVLVV GSKNSSNSNR LAELAQRMGR 

       250        260        270        280        290        300 
TAFLIDDAAD IQEAWVKDAA CVGVTAGASA PDILVQNVIA RLREFGGGEA VTLEGREENI 

       310 
VFEVPKELRV DVREVE

« Hide

References

[1]"Complete genome of a clinical isolate of Salmonella enterica serovar Paratyphi A."
Holt K.E., Thomson N.R., Wain J., Langridge G.C., Hassan R., Bhutta Z., Quail M., Norbertczak H., Danielle W., Simmonds M., White B., Bason N., Mungall K., Dougan G., Parkhill J.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AKU_12601.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FM200053 Genomic DNA. Translation: CAR58157.1.
RefSeqYP_002140894.1. NC_011147.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING554290.SSPA0046.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAR58157; CAR58157; SSPA0046.
GeneID6809326.
KEGGsek:SSPA0046.
PATRIC32339566. VBISalEnt134303_0052.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0761.
HOGENOMHOG000220192.
KOK03527.
OMAQDAMFSL.
ProtClustDBPRK01045.

Enzyme and pathway databases

BioCycSENT554290:GJDA-50-MONOMER.
UniPathwayUPA00056; UER00097.
UPA00059; UER00105.

Family and domain databases

HAMAPMF_00191. IspH.
InterProIPR003451. LytB.
[Graphical view]
PfamPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00216. ispH_lytB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPH_SALPK
AccessionPrimary (citable) accession number: B5BLL3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents