B5BI17 (RNPH_SALPK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	SSPA3349

Organism

Salmonella paratyphi A (strain AKU_12601) [Complete proteome] [HAMAP]

Taxonomic identifier

554290 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella paratyphi A

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP-Rule MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular_function	tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Ribonuclease PH HAMAP-Rule MF_00564		PRO_1000129371

Sequences

	Sequence		Length	Mass (Da)
	B5BI17 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 19008C0C39A840E5	FASTA	238	25,269
	10 20 30 40 50 60 MRPAGRSANQ VRPVTLTRNY TKHAEGSVLV EFGDTKVLCT ASIEEGVPRF LKGQGQGWIT 70 80 90 100 110 120 AEYGMLPRAT HTRNAREAAK GKQGGRTMEI QRLIARALRA AVDLKTLGEF TITLDCDVIQ 130 140 150 160 170 180 ADGGTRTASI TGACVALADA LNKLVANGKL KTNPMKGMVA AVSVGIVNGE AICDLEYVED 190 200 210 220 230 SAAETDMNVV MTEDGRIIEV QGTAEGEPFS HEELLTLLAL ARGGIESIVA TQKAALEN

References

[1]

"Complete genome of a clinical isolate of Salmonella enterica serovar Paratyphi A." Holt K.E., Thomson N.R., Wain J., Langridge G.C., Hassan R., Bhutta Z., Quail M., Norbertczak H., Danielle W., Simmonds M., White B., Bason N., Mungall K., Dougan G., Parkhill J. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AKU_12601.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM200053 Genomic DNA. Translation: CAR61615.1.
RefSeq	YP_002144188.1. NC_011147.1.
3D structure databases
ProteinModelPortal	B5BI17.
SMR	B5BI17. Positions 2-236.
ModBase	Search...
Protein-protein interaction databases
STRING	554290.SSPA3349.
Proteomic databases
PRIDE	B5BI17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAR61615; CAR61615; SSPA3349.
GeneID	6812698.
KEGG	sek:SSPA3349.
PATRIC	32347263. VBISalEnt134303_3821.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
HOGENOM	HOG000229516.
KO	K00989.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	SENT554290:GJDA-3594-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH.
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_SALPK

Accession

Primary (citable) accession number: B5BI17

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program