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Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene

trmD

Organism
Salmonella paratyphi A (strain AKU_12601)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates guanosine-37 in various tRNAs.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei113 – 1131S-adenosyl-L-methionine; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSENT554290:GJDA-2536-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(1)-)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
Gene namesi
Name:trmDUniRule annotation
Ordered Locus Names:SSPA2358
OrganismiSalmonella paratyphi A (strain AKU_12601)
Taxonomic identifieri554290 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001869 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 255255tRNA (guanine-N(1)-)-methyltransferasePRO_1000130206Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi554290.SSPA2358.

Structurei

3D structure databases

ProteinModelPortaliB5BE92.
SMRiB5BE92. Positions 1-246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 1386S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the RNA methyltransferase TrmD family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0336.
HOGENOMiHOG000016242.
OMAiALCLIDS.
OrthoDBiEOG6J48RZ.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.

Sequencei

Sequence statusi: Complete.

B5BE92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFIGIVSLFP EMFRAITDYG VTGRAVKKGL LNIQSWSPRD FAHDRHRTVD
60 70 80 90 100
DRPYGGGPGM LMMVQPLRDA IHAAKAAAGE GAKVIYLSPQ GRKLDQAGVS
110 120 130 140 150
ELATNQKLIL VCGRYEGVDE RVIQAEIDEE WSIGDYVLSG GELPAMTLID
160 170 180 190 200
SVARFIPGVL GHEASAIEDS FADGLLDCPH YTRPEVLEGM EVPPVLLSGN
210 220 230 240 250
HAEIRRWRLK QSLGRTWLRR PELLENLALT EEQARLLAEF KTEHAQQQHK

HDGMA
Length:255
Mass (Da):28,318
Last modified:September 23, 2008 - v1
Checksum:iB96812A32091495F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM200053 Genomic DNA. Translation: CAR60589.1.
RefSeqiYP_002143203.1. NC_011147.1.

Genome annotation databases

EnsemblBacteriaiCAR60589; CAR60589; SSPA2358.
PATRICi32344976. VBISalEnt134303_2698.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM200053 Genomic DNA. Translation: CAR60589.1.
RefSeqiYP_002143203.1. NC_011147.1.

3D structure databases

ProteinModelPortaliB5BE92.
SMRiB5BE92. Positions 1-246.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi554290.SSPA2358.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR60589; CAR60589; SSPA2358.
PATRICi32344976. VBISalEnt134303_2698.

Phylogenomic databases

eggNOGiCOG0336.
HOGENOMiHOG000016242.
OMAiALCLIDS.
OrthoDBiEOG6J48RZ.

Enzyme and pathway databases

BioCyciSENT554290:GJDA-2536-MONOMER.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Pseudogene accumulation in the evolutionary histories of Salmonella enterica serovars Paratyphi A and Typhi."
    Holt K.E., Thomson N.R., Wain J., Langridge G.C., Hasan R., Bhutta Z.A., Quail M.A., Norbertczak H., Walker D., Simmonds M., White B., Bason N., Mungall K., Dougan G., Parkhill J.
    BMC Genomics 10:36-36(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AKU_12601.

Entry informationi

Entry nameiTRMD_SALPK
AccessioniPrimary (citable) accession number: B5BE92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: January 7, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.