B5BAE8 (PEPT_SALPK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidase T
EC=3.4.11.4

Alternative name(s):
Aminotripeptidase
Short name=Tripeptidase
Tripeptide aminopeptidase

Gene names

Name:	pepT
Ordered Locus Names:	SSPA1508

Organism

Salmonella paratyphi A (strain AKU_12601) [Complete proteome] [HAMAP]

Taxonomic identifier

554290 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella paratyphi A

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves the N-terminal amino acid of tripeptides By similarity. HAMAP-Rule MF_00550
Catalytic activity	Release of the N-terminal residue from a tripeptide. HAMAP-Rule MF_00550
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M20B family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptide metabolic process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW tripeptide aminopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Peptidase T HAMAP-Rule MF_00550		PRO_1000129044
Sites
	Active site	80	1	By similarity
	Active site	173	1	Proton acceptor By similarity
	Metal binding	78	1	Zinc 1 By similarity
	Metal binding	140	1	Zinc 1 By similarity
	Metal binding	140	1	Zinc 2 By similarity
	Metal binding	174	1	Zinc 2 By similarity
	Metal binding	196	1	Zinc 1 By similarity
	Metal binding	379	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5BAE8 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: E2C66902B8C698DB	FASTA	409	44,921
	10 20 30 40 50 60 MDKLLERFLH YVSLDTQSKS GVRQVPSTEG QWKLLRLLKQ QLEEMGLVNI TLSEKGTLMA 70 80 90 100 110 120 TLPANVEGDI PAIGFISHVD TSPDFSGKNV NPQIVENYRG GDIALGIGDE VLSPVMFPVL 130 140 150 160 170 180 HQLLGQTLIT TDGKTLLGAD DKAGVAEIMT ALAVLKGNPI PHGEIKVAFT PDEEVGKGAK 190 200 210 220 230 240 HFDVEEFGAQ WAYTVDGGGV GELEFENFNA ASVNIKIVGN NVHPGTAKGV MVNALSLAAR 250 260 270 280 290 300 IHAEVPADEA PETTEGYEGF YHLASMKGTV DRAEMHYIIR DFDRKQFEAR KRKMMEIAKK 310 320 330 340 350 360 VGKGLHPDCY IELVIEDSYY NMREKVVEHP HILDIAQQAM RDCHITPEMK PIRGGTDGAQ 370 380 390 400 LSFMGLPCPN LFTGGYNYHG KHEFVTLEGM EKAVQVIVRI AELTAKRGQ

References

[1]

"Complete genome of a clinical isolate of Salmonella enterica serovar Paratyphi A." Holt K.E., Thomson N.R., Wain J., Langridge G.C., Hassan R., Bhutta Z., Quail M., Norbertczak H., Danielle W., Simmonds M., White B., Bason N., Mungall K., Dougan G., Parkhill J. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AKU_12601.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM200053 Genomic DNA. Translation: CAR59691.1.
RefSeq	YP_002142347.1. NC_011147.1.
3D structure databases
ProteinModelPortal	B5BAE8.
SMR	B5BAE8. Positions 1-408.
ModBase	Search...
Protein-protein interaction databases
STRING	554290.SSPA1508.
Protein family/group databases
MEROPS	M20.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAR59691; CAR59691; SSPA1508.
GeneID	6810819.
KEGG	sek:SSPA1508.
PATRIC	32342998. VBISalEnt134303_1733.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2195.
HOGENOM	HOG000032390.
KO	K01258.
OMA	YVYATIP.
ProtClustDB	PRK05469.
Enzyme and pathway databases
BioCyc	SENT554290:GJDA-1613-MONOMER.
Family and domain databases
HAMAP	MF_00550. Aminopeptidase_M20.
InterPro	IPR001261. ArgE/DapE_CS. IPR002933. Peptidase_M20. IPR011650. Peptidase_M20_dimer. IPR010161. Peptidase_M20B. [Graphical view]
Pfam	PF07687. M20_dimer. 1 hit. PF01546. Peptidase_M20. 1 hit. [Graphical view]
PIRSF	PIRSF037215. Peptidase_M20B. 1 hit.
SUPFAM	SSF55031. Peptidase_M20_dimer. 1 hit.
TIGRFAMs	TIGR01882. peptidase-T. 1 hit.
PROSITE	PS00758. ARGE_DAPE_CPG2_1. 1 hit. PS00759. ARGE_DAPE_CPG2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEPT_SALPK

Accession

Primary (citable) accession number: B5BAE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program