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B5AR80

- OXLA_BOTPA

UniProt

B5AR80 - OXLA_BOTPA

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Protein

L-amino-acid oxidase

Gene
N/A
Organism
Bothrops pauloensis (Neuwied's lancehead) (Bothrops neuwiedi pauloensis)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids (highly against L-Met, L-Leu, L-Phe and L-Ile), thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as antibacterial on both Gram-positive and Gram-negative bacteria and antiparasitic activities, as well as induction of platelet aggregation. Effects of snake L-amino oxidases on platelets are controversial, since they either induce aggregation or inhibit agonist-induced aggregation. These different effects are probably due to different experimental conditions. This protein may also have activities in hemorrhage, hemolysis, edema, and apoptosis.1 Publication

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

FAD By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891FAD By similarity
Binding sitei108 – 1081Substrate By similarity
Binding sitei241 – 2411Substrate By similarity
Binding sitei279 – 2791FAD; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei390 – 3901Substrate By similarity
Binding sitei475 – 4751FAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi61 – 622FAD By similarity
Nucleotide bindingi81 – 822FAD By similarity
Nucleotide bindingi105 – 1084FAD By similarity
Nucleotide bindingi482 – 4876FAD By similarity
Nucleotide bindingi482 – 4832Substrate By similarity

GO - Molecular functioni

  1. L-amino-acid oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. defense response to bacterium Source: UniProtKB-KW
  3. hemolysis in other organism Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Hemostasis impairing toxin, Oxidoreductase, Platelet aggregation activating toxin, Toxin

Keywords - Biological processi

Apoptosis, Cytolysis, Hemolysis

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
L-amino-acid oxidase (EC:1.4.3.2)
Short name:
Bp-LAAO
Short name:
LAAO
Short name:
LAO
OrganismiBothrops pauloensis (Neuwied's lancehead) (Bothrops neuwiedi pauloensis)
Taxonomic identifieri1042543 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18182 PublicationsAdd
BLAST
Chaini19 – ›503›485L-amino-acid oxidasePRO_0000412598Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 191 By similarity
Glycosylationi190 – 1901N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi349 ↔ 430 By similarity

Post-translational modificationi

N-glycosylated Inferred. The enzymatic activity is not affected by deglycosylation.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Homodimer; non-covalently linked.1 Publication

Structurei

3D structure databases

ProteinModelPortaliB5AR80.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG005729.

Family and domain databases

InterProiIPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
PRINTSiPR00757. AMINEOXDASEF.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

B5AR80-1 [UniParc]FASTAAdd to Basket

« Hide

MNVFFMFSLL FLAALGSCAD DGNPLEECFR ETDYEEFLEI AKNGLSATSN    50
PKHVVIVGAG MSGLSAAYVL ANAGHQVTVL EASKRAGGRV RTYRNDKEGW 100
YANLGPMRLP EKHRIVREYI RKFGLQLNEF SQENENAWYF IKNIRKRVGE 150
VNKDPGVLEY PVKPSEVGKS AGQLYEESLQ KAVEELRRTN CSYMLNKYDT 200
YSTKEYLLKE GNLSPGAVDM IGDLLNEDSG YYVSFIESLK HDDIFAYEKR 250
FDEIVGGMDK LPTSMYQAIQ EKVRLNVRVI KIQQDVKEVT VTYQTSAKET 300
LSVTADYVIV CTTSRAARRI KFEPPLPPKK AHALRSVHYR SGTKIFLTCT 350
KKFWEDDGIH GGKSTTDLPS RFIYYPNHNF PSGVGVIIAY GIGDDANFFQ 400
ALDFKDCGDI VINDLSLIHQ LPKEEIQAFC RPSMIQRWSL DKYAMGGITT 450
FTPYQFQHFS EALTAPVDRI YFAGEYTAQA HGWIDSTIKS GLTAARDVNR 500
ASE 503
Length:503
Mass (Da):56,799
Last modified:September 23, 2008 - v1
Checksum:i800568E8C2166CD0
GO

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei503 – 5031

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EU870608 mRNA. Translation: ACG55578.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EU870608 mRNA. Translation: ACG55578.1 .

3D structure databases

ProteinModelPortali B5AR80.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG005729.

Family and domain databases

InterProi IPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
PRINTSi PR00757. AMINEOXDASEF.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-68, FUNCTION, SUBUNIT, GLYCOSYLATION.
    Tissue: Venom and Venom gland.
  2. "Combined snake venomics and venom gland transcriptomic analysis of Bothropoides pauloensis."
    Rodrigues R.S., Boldrini-Franca J., Fonseca F.P., de la Torre P., Henrique-Silva F., Sanz L., Calvete J.J., Rodrigues V.M.
    J. Proteomics 75:2707-2720(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 19-34, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Venom.

Entry informationi

Entry nameiOXLA_BOTPA
AccessioniPrimary (citable) accession number: B5AR80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 23, 2008
Last modified: February 19, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Has parasiticidal activities against leishmania, as a result of enzyme-catalyzed hydrogen peroxide production (1 Publication).

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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