B5A5T4 (QVR_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein quiver

Alternative name(s):
Protein sleepless

Gene names

Name:	qvr
Synonyms:	sss
ORF Names:	CG33472

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for homeostatic regulation of sleep under normal conditions and after sleep deprivation. Important regulator of the Sh K+ channel, acting as a signaling molecule that connects sleep drive to lowered membrane excitability, possibly by enhancing K+ channel activity and thus reducing neuronal excitability. Ref.1
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Ref.1.
Tissue specificity	Enriched in brain and head. Ref.1
Post-translational modification	N-glycosylated. Ref.1
Disruption phenotype	Both daytime and nighttime sleep are severely reduced in both males and females. A small percentage of flies (around 9%) do not sleep at all. A moderate reduction has minimal effects on baseline sleep but markedly reduces the amount of recovery sleep after sleep deprivation. Mutants have impaired Sh-dependent K+ current. Ref.1
Sequence similarities	Belongs to the quiver family.
Sequence caution	The sequence AAL90233.1 differs from that shown. Reason: Several sequencing errors.

Ontologies

Keywords
Biological process	Biological rhythms
Cellular component	Cell membrane Membrane
Domain	Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of circadian sleep/wake cycle, sleep Inferred from mutant phenotype Ref.1. Source: FlyBase regulation of synaptic transmission, cholinergic Inferred from mutant phenotype PubMed 20010822. Source: FlyBase
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW external side of plasma membrane Inferred from direct assay Ref.1. Source: FlyBase
Molecular_function	GPI anchor binding Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 127	95	Protein quiver		PRO_0000359760
	Propeptide	128 – 158	31	Removed in mature form		PRO_0000359761
Amino acid modifications
	Lipidation	127	1	GPI-anchor amidated asparagine Probable
	Glycosylation	57	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	110 – 117	8	MESSGNGH → VEGSGSGR in ACF58241. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	B5A5T4 [UniParc]. Last modified September 21, 2011. Version 2. Checksum: BF3E8327EC40A58F	FASTA	158	18,124
	10 20 30 40 50 60 MWTQRNAVGN WLLVLTAVIG FLTFIWIPQT SAECQTRSIY CYECDSWTDA RCKDPFNYTA 70 80 90 100 110 120 LPRDQPPLMT CNGCCVKMVR HQRSPYEVVR RMCTSQLQIN LFMVDHVCMM ESSGNGHMCF 130 140 150 CEEDMCNSSK NLHTNGCQLH LIPIAVAVSW LMGQLLSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of SLEEPLESS, a sleep-promoting factor." Koh K., Joiner W.J., Wu M.N., Yue Z., Smith C.J., Sehgal A. Science 321:372-376(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GPI-ANCHOR, GLYCOSYLATION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Sleepless nights - Issue 101 of January 2009

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU816195 mRNA. Translation: ACF58241.1. AE013599 Genomic DNA. Translation: ACL83100.1. AY089495 mRNA. Translation: AAL90233.1. Sequence problems.
RefSeq	NP_001137646.1. NM_001144174.2.
UniGene	Dm.20865.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	7227.FBpp0113067.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0114575; FBpp0113067; FBgn0260499.
GeneID	2768718.
KEGG	dme:Dmel_CG33472.
Organism-specific databases
CTD	2768718.
FlyBase	FBgn0260499. qvr.
Phylogenomic databases
eggNOG	NOG74387.
InParanoid	B5A5T4.
OrthoDB	EOG42RBQW.
PhylomeDB	B5A5T4.
Gene expression databases
Bgee	B5A5T4.
Family and domain databases
ProtoNet	Search...
Other
ChiTaRS	qvr. drosophila.
GenomeRNAi	2768718.
NextBio	848161.

Entry information

Entry name

QVR_DROME

Accession

Primary (citable) accession number: B5A5T4
Secondary accession number(s): A1Z8M0, B7YZF3, Q8SXP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	September 21, 2011
Last modified:	April 3, 2013
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project