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Protein

Endo-1,4-beta-xylanase A

Gene

xynAS9

Organism
Streptomyces sp.
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to hydrolysis of hemicellulose, the major component of plant cell-walls. Hydrolyzes xylan to xylose and xylobiose.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

Cofactori

Note: Does not require any standard metal (Mg2+, Mn2(+), Ca2+).

Enzyme regulationi

Completely inhibited by Hg2+, unaffected by EDTA.1 Publication

Kineticsi

    1. Vmax=772.20 µmol/min/mg enzyme for oat spelt xylan1 Publication
    2. Vmax=490.87 µmol/min/mg enzyme for birchwood xylan1 Publication

    pH dependencei

    Optimum pH is 6.5, more than 80% active between pH 5.0 and 7.0.1 Publication

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius, more than 80% of activity remains after 1 hour at 60 degrees Celsius.1 Publication

    Pathwayi: xylan degradation

    This protein is involved in the pathway xylan degradation, which is part of Glycan degradation.
    View all proteins of this organism that are known to be involved in the pathway xylan degradation and in Glycan degradation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei166 – 1661Proton donorBy similarity
    Active sitei271 – 2711NucleophileBy similarity

    GO - Molecular functioni

    • endo-1,4-beta-xylanase activity Source: UniProtKB
    • polysaccharide binding Source: InterPro

    GO - Biological processi

    • xylan catabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Enzyme and pathway databases

    BRENDAi3.2.1.8. 11186.
    UniPathwayiUPA00114.

    Protein family/group databases

    CAZyiCBM2. Carbohydrate-Binding Module Family 2.
    GH10. Glycoside Hydrolase Family 10.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-1,4-beta-xylanase A (EC:3.2.1.8)
    Short name:
    Xylanase A
    Gene namesi
    Name:xynAS9
    OrganismiStreptomyces sp.
    Taxonomic identifieri1931 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Sequence analysisAdd
    BLAST
    Chaini34 – 464431Endo-1,4-beta-xylanase APRO_0000424416Add
    BLAST

    Structurei

    Secondary structure

    1
    464
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi43 – 486Combined sources
    Turni49 – 513Combined sources
    Beta strandi53 – 586Combined sources
    Helixi60 – 623Combined sources
    Helixi66 – 7510Combined sources
    Beta strandi77 – 837Combined sources
    Helixi87 – 904Combined sources
    Helixi100 – 11112Combined sources
    Beta strandi115 – 1228Combined sources
    Beta strandi124 – 1263Combined sources
    Helixi130 – 1334Combined sources
    Helixi136 – 1383Combined sources
    Helixi139 – 15315Combined sources
    Turni154 – 1574Combined sources
    Beta strandi159 – 1657Combined sources
    Beta strandi172 – 1743Combined sources
    Helixi178 – 1836Combined sources
    Helixi187 – 19812Combined sources
    Beta strandi200 – 21011Combined sources
    Beta strandi212 – 2154Combined sources
    Helixi216 – 23116Combined sources
    Beta strandi237 – 2404Combined sources
    Beta strandi243 – 2453Combined sources
    Helixi253 – 26210Combined sources
    Beta strandi266 – 27914Combined sources
    Helixi282 – 30120Combined sources
    Beta strandi305 – 31410Combined sources
    Helixi315 – 3173Combined sources
    Helixi320 – 3234Combined sources
    Beta strandi331 – 3333Combined sources
    Helixi341 – 35010Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3WUBX-ray2.08A39-351[»]
    3WUEX-ray2.15A39-351[»]
    3WUFX-ray2.04A39-351[»]
    3WUGX-ray1.88A39-351[»]
    ProteinModelPortaliB4XVN1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 349310GH10PROSITE-ProRule annotationAdd
    BLAST
    Domaini354 – 457104CBM2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CBM2 (carbohydrate binding type-2) domain.PROSITE-ProRule annotation
    Contains 1 GH10 (glycosyl hydrolase family 10) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.40.290. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR008965. Carb-bd_dom.
    IPR001919. CBD2.
    IPR012291. CBD_carb-bd_dom.
    IPR001000. GH10.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00553. CBM_2. 1 hit.
    PF00331. Glyco_hydro_10. 1 hit.
    [Graphical view]
    PRINTSiPR00134. GLHYDRLASE10.
    SMARTiSM00637. CBD_II. 1 hit.
    SM00633. Glyco_10. 1 hit.
    [Graphical view]
    SUPFAMiSSF49384. SSF49384. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS51173. CBM2. 1 hit.
    PS51760. GH10_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B4XVN1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MFRHHPTRGR RTAGLLAAAL ATLSAGLTAV APAHPARADT ATLGELAEAK
    60 70 80 90 100
    GRYFGSATDN PELPDTQYTQ ILGSEFSQIT VGNTMKWQYT EPSRGRFDYT
    110 120 130 140 150
    AAEEIVDLAE SNGQSVRGHT LVWHNQLPSW VDDVPAGELL GVMRDHITHE
    160 170 180 190 200
    VDHFKGRLIH WDVVNEAFEE DGSRRQSVFQ QKIGDSYIAE AFKAARAADP
    210 220 230 240 250
    DVKLYYNDYN IEGIGPKSDA VYEMVKSFKA QGIPIDGVGM QAHLIAGQVP
    260 270 280 290 300
    ASLQENIRRF ADLGVDVALT ELDIRMTLPR TAAKDAQQAT DYGAVVEACL
    310 320 330 340 350
    VVSRCVGITV WDYTDKYSWV PSVFPGQGAA LPWDEDFAKK PAYHAIAAAL
    360 370 380 390 400
    NGGSPAPGGN CTATYRVTSQ WQGGFTAEIT VGNDHTAPIT GWTVTWTLSS
    410 420 430 440 450
    GQSISHMWNG NLTVNGQDVT VRDVGYNGTL GGNGSTTFGF QGEGVADTPA
    460
    DVTCTPGRPS GTSA
    Length:464
    Mass (Da):49,868
    Last modified:September 23, 2008 - v1
    Checksum:i4856950BC604B95C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    EU153378 Genomic DNA. Translation: ABX71815.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    EU153378 Genomic DNA. Translation: ABX71815.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3WUBX-ray2.08A39-351[»]
    3WUEX-ray2.15A39-351[»]
    3WUFX-ray2.04A39-351[»]
    3WUGX-ray1.88A39-351[»]
    ProteinModelPortaliB4XVN1.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    CAZyiCBM2. Carbohydrate-Binding Module Family 2.
    GH10. Glycoside Hydrolase Family 10.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    UniPathwayiUPA00114.
    BRENDAi3.2.1.8. 11186.

    Family and domain databases

    Gene3Di2.60.40.290. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR008965. Carb-bd_dom.
    IPR001919. CBD2.
    IPR012291. CBD_carb-bd_dom.
    IPR001000. GH10.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00553. CBM_2. 1 hit.
    PF00331. Glyco_hydro_10. 1 hit.
    [Graphical view]
    PRINTSiPR00134. GLHYDRLASE10.
    SMARTiSM00637. CBD_II. 1 hit.
    SM00633. Glyco_10. 1 hit.
    [Graphical view]
    SUPFAMiSSF49384. SSF49384. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS51173. CBM2. 1 hit.
    PS51760. GH10_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. "Cloning, expression, and characterization of a new xylanase with broad temperature adaptability from Streptomyces sp. S9."
      Li N., Meng K., Wang Y., Shi P., Luo H., Bai Y., Yang P., Yao B.
      Appl. Microbiol. Biotechnol. 80:231-240(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A XYLANASE, LACK OF COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: S9.

    Entry informationi

    Entry nameiXYNA_STRSQ
    AccessioniPrimary (citable) accession number: B4XVN1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2013
    Last sequence update: September 23, 2008
    Last modified: June 8, 2016
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.