Reviewed, UniProtKB/Swiss-Prot B4ULC6 (ISPE_ANASK)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	AnaeK_0130

Organism

Anaeromyxobacter sp. (strain K) [Complete proteome] [HAMAP]

Taxonomic identifier

447217 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity.
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000092060
Regions
	Nucleotide binding	95 – 105	11	ATP Potential
Sites
	Active site	10	1	By similarity
	Active site	136	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4ULC6-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: F946B0458983D5CA	FASTA	313	32,878
	10 20 30 40 50 60 MRLVTLAPAK VNLVLRVGPV RADGYHDLRT LMVPLDLGDR VDVRVSARPG PVRCTVPGRP 70 80 90 100 110 120 ELDGPENLAA RAAEAFRRRF GVDRAVSIRI EKRTPVTAGL GGGSSDAAAV LRCLARALRV 130 140 150 160 170 180 RDGAALAALA LEIGSDVPFF LGPGPAWAAG RGERLSRAEV PPLDLVLVYP ADPSLAIRAG 190 200 210 220 230 240 DAYRWLDEAR ASGSQAPRRL GRPGRWRPTL LGNDLQAPCV ARKPALQALL GLLVGAGATA 250 260 270 280 290 300 AIMSGSGPTV FGIFPGRGAA RGAALAIQGR AKGGAAGVQV LLARTVRRHP RVSPWRSPRS 310 ASSRSTRRSS RPT

References

[1]

"Complete sequence of Anaeromyxobacter sp. K." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikiva G., Beliaev A. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001131 Genomic DNA. Translation: ACG71373.1.
RefSeq	YP_002132502.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6786719.
GenomeReviews	Gene locus AnaeK_0130 in contig CP001131_GR.
KEGG	ank:AnaeK_0130.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PAVMVNP.
Family and domain databases
HAMAP	MF_00061. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_ANASK

Accession

Primary (citable) accession number: B4ULC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)