B4UKM8 (BIOD_ANASK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	AnaeK_0076

Organism

Anaeromyxobacter sp. (strain K) [Complete proteome] [HAMAP]

Taxonomic identifier

447217 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_1000119854
Regions
	Nucleotide binding	111 – 114	4	ATP By similarity
	Nucleotide binding	171 – 172	2	ATP By similarity
Sites
	Metal binding	16	1	Magnesium 2 By similarity
	Metal binding	50	1	Magnesium 2 By similarity
	Metal binding	111	1	Magnesium 2 By similarity
	Binding site	41	1	Substrate By similarity
	Binding site	50	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4UKM8 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: F2091B92B5A45C8F	FASTA	223	23,021
	10 20 30 40 50 60 MRGLFVTGTD TGVGKTEVSC ALLRAARAAG LDAVGMKPAQ SGHVPGEPSD AERLREASDC 70 80 90 100 110 120 VEPLEAICPY TFAAPLAPAA AARLEGREVS LARVVEVARA LAARHAAVVV EGAGGLLVPL 130 140 150 160 170 180 TARETHADLA AALGLPVLVV ARAGLGTVNH TALTVEALER RGLAVAGIVL NRTGPEDDPS 190 200 210 220 VPLNPAEIAR LTHREPIALL PWEPDIARRA RSLGSILGAK IQF

References

[1]

"Complete sequence of Anaeromyxobacter sp. K." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikiva G., Beliaev A. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001131 Genomic DNA. Translation: ACG71319.1.
RefSeq	YP_002132448.1. NC_011145.1.
3D structure databases
ProteinModelPortal	B4UKM8.
ModBase	Search...
Protein-protein interaction databases
STRING	B4UKM8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6784958.
GenomeReviews	Gene locus AnaeK_0076 in contig CP001131_GR.
KEGG	ank:AnaeK_0076.
PATRIC	20934716. VBIAnaSp90767_0078.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	IDTGIGK.
ProtClustDB	CLSK944803.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_ANASK

Accession

Primary (citable) accession number: B4UKM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program