B4UIX7 (PURL_ANASK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	AnaeK_4346

Organism

Anaeromyxobacter sp. (strain K) [Complete proteome] [HAMAP]

Taxonomic identifier

447217 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000194816
Regions
	Nucleotide binding	103 – 114	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B4UIX7 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: DA92447D4ADC1BD8	FASTA	759	80,494
	10 20 30 40 50 60 MTEPITPEIV AQHGLKPDEY QRILEHLGRT PTLTELGVFS VMWSEHCSYK SSRVHLKTFP 70 80 90 100 110 120 TSGPRVLQGP GENAGVVDLG DGLAAAFKME SHNHPSYIEP YQGAATGVGG ILRDVFTMGA 130 140 150 160 170 180 RPIASLNALR FGDPSHPRTA YLLEGVVAGI GGYGNCMGVP TVGGEVAFHP SYNGNCLVNA 190 200 210 220 230 240 FTLGILPADK IFRGTAAGVG NPVMYVGAKT GRDGIHGATM ASAEFDASTE EKRPTVQVGD 250 260 270 280 290 300 PFMEKLLLEA CLELFQTDAV VGIQDMGAAG LTSSSVEMAG RGGNGLDLFL DQVPLREEGM 310 320 330 340 350 360 TPYEILLSES QERMLLVAAE GKEELVRSIC EKWDLDVAVI GRVTASGRWR AHWRGAVVAD 370 380 390 400 410 420 LPVDPLTEGA PKYHRPMTPH PALPALHAFD LATLPEPADL GAALVRLLAR PTIASKEWVY 430 440 450 460 470 480 RQYDHMVRLV GAVRPGGDAA VVRLAVSHDA HAHKGIALSV GVNGRFCFLD PYLGAMHAVA 490 500 510 520 530 540 ECARNIACVG GEPIAITDCL NFGNPEKPEI MWQFAECVRG IGDACRAFGT PVVSGNVSLY 550 560 570 580 590 600 NETEGQGILP TPTVGMVGLV EPVERTCHST FRDAGDVIAL VGSLQGEVGG SEYLSAEHGK 610 620 630 640 650 660 EAGRPPALDL AREKAVQETV RRAVRAGLLS SAHDCSEGGL AVALAESCMM HEVPSDGSKP 670 680 690 700 710 720 AWIGCAVRIP FPVRKDFVLF GEDASRILVS LPKENAARFV DLAQQCGAPV IRLGAVGGDR 730 740 750 LEIQGALSVP VEELARAWRD GIPAVLRRDA AHAGATAPV

References

[1]

"Complete sequence of Anaeromyxobacter sp. K." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikiva G., Beliaev A. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001131 Genomic DNA. Translation: ACG75549.1.
RefSeq	YP_002136678.1. NC_011145.1.
3D structure databases
ProteinModelPortal	B4UIX7.
ModBase	Search...
Protein-protein interaction databases
STRING	B4UIX7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6784361.
GenomeReviews	Gene locus AnaeK_4346 in contig CP001131_GR.
KEGG	ank:AnaeK_4346.
PATRIC	20943396. VBIAnaSp90767_4364.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_ANASK

Accession

Primary (citable) accession number: B4UIX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program