Reviewed, UniProtKB/Swiss-Prot B4UI29 (ILVD_ANASK)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	AnaeK_4240

Organism

Anaeromyxobacter sp. (strain K) [Complete proteome] [HAMAP]

Taxonomic identifier

447217 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000089366
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	192	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	B4UI29-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: E4AD0B84E7A6C871	FASTA	560	59,428
	10 20 30 40 50 60 MRSDRIKKGF ERAPHRSLLR ATGLNDGDFE KPFIGIANSH IDIIPGHYYL QEYGRIAKDE 70 80 90 100 110 120 IRKAGGVPFE FNTIGVDDGI AMGHEGMKYS LPSRELIADS IETVMNAHQL DALVCIPNCD 130 140 150 160 170 180 KIVPGMLMGA LRVNVPTVFV SGGPMKAGHL HDGTPIDLNT AFEAVGKRAQ GQLTDAELYE 190 200 210 220 230 240 IECQACPSGG SCSGMFTANS MNVLCEAMGV ALPGNGTVLA LTPEREALVR RAARRAVEIA 250 260 270 280 290 300 ADERFKLRNI ANRDAIHNAM VVDMAMGGSS NTVLHMLAIS REAGAPLSLR DIEEIAGKVA 310 320 330 340 350 360 HIAKIAPSLA TVHMEDIHRA GGVPAVLREA ARRGGSVREG ALTVTGETVG ERIRDARTAD 370 380 390 400 410 420 PELIRPLENA YSPVGGLAVL FGNLAAEGAV VKTAGIQPSM RRFTGAAICF DSQDEAIAGI 430 440 450 460 470 480 MGGKVKPGHF VVIRYEGPKG GPGMQEMLSP TSLIMGMGLG ESVALVTDGR FSGATRGACV 490 500 510 520 530 540 GHVSPEAAEG GVIGLVQDGD RITIDVEARA LTVDVPDAEL ARRREGFRPK RRDPGSSWLR 550 560 RYAHLVTNAA NGAVLRSTDL

References

[1]

"Complete sequence of Anaeromyxobacter sp. K." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikiva G., Beliaev A. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001131 Genomic DNA. Translation: ACG75443.1.
RefSeq	YP_002136572.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6784255.
GenomeReviews	Gene locus AnaeK_4240 in contig CP001131_GR.
KEGG	ank:AnaeK_4240.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SRKVPCL.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_ANASK

Accession

Primary (citable) accession number: B4UI29

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)