B4UHF5 (DXS_ANASK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	AnaeK_1157

Organism

Anaeromyxobacter sp. (strain K) [Complete proteome] [HAMAP]

Taxonomic identifier

447217 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	636 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 636	636	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000115719

Sequences

	Sequence		Length	Mass (Da)
	B4UHF5 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: CE0B195F3349A0EF	FASTA	636	67,798
	10 20 30 40 50 60 MGRLLDTIDS PTDLKKVPVE QLPALCQEIR EQIIQTCARN GGHLGSSLGA VEINVALHHV 70 80 90 100 110 120 FSSPQDKLVW DVGHQAYAHK LLTGRREAFR TIRTEGGLAG FPERHESAHD AFGVGHASTA 130 140 150 160 170 180 ISAALGMIEA KRVTGEPGKV VAVVGDGAMT GGVAFEGLNQ AGYLGRNLLV VLNDNEMSIS 190 200 210 220 230 240 PNVGALSEWF SKKFASRTYN RWRRQVKEFL ESVPKGPEAI EIIRHGINAT KALVTPGILF 250 260 270 280 290 300 EGLGFHYVGP VDGHDVKGLV ETFQKLAIFD GPVLLHAITT KGKGYHPAES DKATRGHGLS 310 320 330 340 350 360 FFDVATGKPV KKSPGAKAYT DLFAEALCEE MEHDPRVVAI TAAMLEGTGL IKAKQRFPDR 370 380 390 400 410 420 TYDVGIAEQH AVTFAAGLAC EGIRPVVAIY STFLQRAYDQ IIHDVALQKL PVTFALDRGG 430 440 450 460 470 480 LVGADGKTHQ GAFDLAYLRC VPGLVVMAPS DENELRHMLH TSLQHEGPAA LRYPRGAGEG 490 500 510 520 530 540 VALEPARVLE IGKGRLVRNV PGKPDVCVVA AGTTLKAALA AAEALAAEGV AATVVDPRFV 550 560 570 580 590 600 KPLDEELICA EAARAKRVVT VEEGCLAGGF GTACLEAFER RGLLEAGLGV RRLGIPDEFI 610 620 630 THAEQAKQRA WVGIDADAIA AACRALVGDR KARGVA

References

[1]

"Complete sequence of Anaeromyxobacter sp. K." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikiva G., Beliaev A. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001131 Genomic DNA. Translation: ACG72390.1.
RefSeq	YP_002133519.1. NC_011145.1.
3D structure databases
ProteinModelPortal	B4UHF5.
ModBase	Search...
Protein-protein interaction databases
STRING	B4UHF5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6787688.
GenomeReviews	Gene locus AnaeK_1157 in contig CP001131_GR.
KEGG	ank:AnaeK_1157.
PATRIC	20936914. VBIAnaSp90767_1170.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	IMEYAYL.
ProtClustDB	CLSK2465121.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_ANASK

Accession

Primary (citable) accession number: B4UHF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program