B4UG87 (SYGB_ANASK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase beta subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name=GlyRS

Gene names

Name:	glyS
Ordered Locus Names:	AnaeK_2554

Organism

Anaeromyxobacter sp. (strain K) [Complete proteome] [HAMAP]

Taxonomic identifier

447217 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	701 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP-Rule MF_00255
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro glycine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 701	701	Glycine--tRNA ligase beta subunit HAMAP-Rule MF_00255		PRO_1000197168

Sequences

	Sequence		Length	Mass (Da)
	B4UG87 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 391A2CA4F2EA58BF	FASTA	701	75,745
	10 20 30 40 50 60 MADLLFEIGA EEIPAGFVPG ALRQLEDDLA KALADARLAH GEVRSVGTPR RLAVWARDVA 70 80 90 100 110 120 PKQTDARTEA FGPPVAQAYD AEGKPTPAAT GFARSQGVEV SALVRAQTPK GERVAVTKVE 130 140 150 160 170 180 KGRRAEQVLP ALLERLVGGL RFRKAMRSRF DEATFARPVR WMVALLGGRP LKVRHGEVTS 190 200 210 220 230 240 GKVTYGHRFL APKAIALKGT PDDYLAKLRR AHVLADPVER RAALLAELAR AGKEAAGKVR 250 260 270 280 290 300 EDPALVEQVL YLVEEPTAVV GEFEKSNLEL PPEVVISEMR NHQRYFAVVD GKGRLKNRFV 310 320 330 340 350 360 AVSATRVKDP AVARHGYERV LRARLADARF FFEEDRKRKL HERIEDLGRR TFQAKLGSEL 370 380 390 400 410 420 DRAQRIGAVA SALARALGKD ALVADLLEAS RLAKVDLNTG MVGEFPELQG TMGAHYARLE 430 440 450 460 470 480 GLKPEIADAI EDHYKPIGAA EELPRSDLGA LVAVADRLHS LVGIIGVGEK ATGAADPFGL 490 500 510 520 530 540 RRAAIGILRI VIARGYHLSL AAAVEQTLDA LSGVKLAAGR ALVAEQVLDF LRGRVRAAWT 550 560 570 580 590 600 ERFDADLVEA VLAAGSDDVV DARRRLEALA DAKARPDFGS LAVAFKRVAN IQEKAGGSGA 610 620 630 640 650 660 AAVDPALLRD AAEKDLLAAL EKVEQEVVAR RAARDYPAVL RTVATLEPAV ARFFDGVLVM 670 680 690 700 AEDPALRANR LGLMRRVAAL FSDLADFRKI QAEAPAQARA G

References

[1]

"Complete sequence of Anaeromyxobacter sp. K." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikiva G., Beliaev A. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001131 Genomic DNA. Translation: ACG73779.1.
RefSeq	YP_002134908.1. NC_011145.1.
3D structure databases
ProteinModelPortal	B4UG87.
ModBase	Search...
Protein-protein interaction databases
STRING	447217.AnaeK_2554.
Proteomic databases
PRIDE	B4UG87.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACG73779; ACG73779; AnaeK_2554.
GeneID	6785705.
KEGG	ank:AnaeK_2554.
PATRIC	20939777. VBIAnaSp90767_2578.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0751.
HOGENOM	HOG000264302.
KO	K01879.
OMA	GMHYARL.
ProtClustDB	PRK01233.
Enzyme and pathway databases
BioCyc	ASP447217:GHB0-2586-MONOMER.
Family and domain databases
HAMAP	MF_00255. Gly_tRNA_synth_beta.
InterPro	IPR008909. DALR_anticod-bd. IPR015944. Gly-tRNA-synth_bsu. IPR006194. Gly-tRNA-synth_heterodimer. [Graphical view]
Pfam	PF05746. DALR_1. 1 hit. PF02092. tRNA_synt_2f. 1 hit. [Graphical view]
PRINTS	PR01045. TRNASYNTHGB.
SMART	SM00836. DALR_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00211. glyS. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGB_ANASK

Accession

Primary (citable) accession number: B4UG87

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program