B4UDZ3 (PANB_ANASK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase
EC=2.1.2.11

Alternative name(s):
Ketopantoate hydroxymethyltransferase
Short name=KPHMT

Gene names

Name:	panB
Ordered Locus Names:	AnaeK_3740

Organism

Anaeromyxobacter sp. (strain K) [Complete proteome] [HAMAP]

Taxonomic identifier

447217 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity. HAMAP MF_00156
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00156
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity. HAMAP MF_00156
Subcellular location	Cytoplasm Potential HAMAP MF_00156.
Sequence similarities	Belongs to the PanB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	3-methyl-2-oxobutanoate hydroxymethyltransferase HAMAP MF_00156		PRO_1000096939
Regions
	Region	53 – 54	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	195	1	Proton acceptor By similarity
	Metal binding	53	1	Magnesium By similarity
	Metal binding	96	1	Magnesium By similarity
	Metal binding	128	1	Magnesium By similarity
	Binding site	96	1	Alpha-ketoisovalerate By similarity
	Binding site	126	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4UDZ3 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: EE4D6C07B3BF21FB	FASTA	306	32,293
	10 20 30 40 50 60 MSSHPPAPRK HVTIHELRRM KESGERIAMV TAYDATAARL VAVAGVDAVL VGDSLGMAVQ 70 80 90 100 110 120 GHESTLPVTL DQMVYHSAMV RRGLARGDGR AHLVTDMSFG SYQASADEAV KAAMRLVAEG 130 140 150 160 170 180 GAEAVKLEGG AEFGEVIRRI VRAGVPVMGH IGLTPQSVHK MGGYVVQGKD SEKAQQILRD 190 200 210 220 230 240 ARALEAAGCY ALVLECIPSE LARIVTSQLR VPTIGIGAGP HCDGQVLVLN DLLGLDASFT 250 260 270 280 290 300 PRFVKRFGEV GAAVQDAVGA YVGEVKARAF PDDAHSFHSS SVRLVPVERH AEAAEEEPPD AIGAPI

References

[1]

"Complete sequence of Anaeromyxobacter sp. K." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikiva G., Beliaev A. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001131 Genomic DNA. Translation: ACG74951.1.
RefSeq	YP_002136080.1. NC_011145.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B4UDZ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6787429.
GenomeReviews	Gene locus AnaeK_3740 in contig CP001131_GR.
KEGG	ank:AnaeK_3740.
PATRIC	20942229. VBIAnaSp90767_3784.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG299908.
OMA	YDATFAH.
ProtClustDB	PRK00311.
Family and domain databases
HAMAP	MF_00156. PanB. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
KO	K00606.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR00222. PanB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB_ANASK

Accession

Primary (citable) accession number: B4UDZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program