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B4UCB2

- BIOB_ANASK

UniProt

B4UCB2 - BIOB_ANASK

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Protein

Biotin synthase

Gene
bioB, AnaeK_3535
Organism
Anaeromyxobacter sp. (strain K)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi110 – 1101Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi143 – 1431Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi203 – 2031Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciASP447217:GHB0-3586-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:AnaeK_3535
OrganismiAnaeromyxobacter sp. (strain K)
Taxonomic identifieri447217 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeAnaeromyxobacter
ProteomesiUP000001871: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Biotin synthaseUniRule annotationPRO_0000381201Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi447217.AnaeK_3535.

Structurei

3D structure databases

ProteinModelPortaliB4UCB2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B4UCB2-1 [UniParc]FASTAAdd to Basket

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MCETASTTLP PGITPISGDE ARRLIHHTSG PELEALLDRA EAVRRAVHGD    50
EVALCGITNA KSGRCPEDCG FCSQSARFEG ADAPVYPMIG AGEIVEQAHK 100
AERAGAREFS IVASGTRLAR EQELATVEEA LRRLRAETAV EPCASLGLMR 150
EPELRRLKDA GLMHYHHNLE TARSHFENVC TTHTFDEQLE TIRAAKGLGL 200
KLCSGGILGM GETPEQRVEF AEEVRDLGVD CVPVNFLNPR AGTPLAHLKA 250
ITPEECLAAL AVFRLMMPAA HIFVMGGREV NLGDRQDLIF RAGANGTMVG 300
NYLTSAGRAP DLTVGMVERQ GLTLRPPDTG KPWAFDGHAP SDADWNRKAA 350
EPRPRPLPVV R 361
Length:361
Mass (Da):39,090
Last modified:September 23, 2008 - v1
Checksum:i4903FCCEBB8285D8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001131 Genomic DNA. Translation: ACG74748.1.
RefSeqiWP_012527517.1. NC_011145.1.
YP_002135877.1. NC_011145.1.

Genome annotation databases

EnsemblBacteriaiACG74748; ACG74748; AnaeK_3535.
GeneIDi6784665.
KEGGiank:AnaeK_3535.
PATRICi20941814. VBIAnaSp90767_3579.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001131 Genomic DNA. Translation: ACG74748.1 .
RefSeqi WP_012527517.1. NC_011145.1.
YP_002135877.1. NC_011145.1.

3D structure databases

ProteinModelPortali B4UCB2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 447217.AnaeK_3535.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACG74748 ; ACG74748 ; AnaeK_3535 .
GeneIDi 6784665.
KEGGi ank:AnaeK_3535.
PATRICi 20941814. VBIAnaSp90767_3579.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci ASP447217:GHB0-3586-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K.

Entry informationi

Entry nameiBIOB_ANASK
AccessioniPrimary (citable) accession number: B4UCB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 23, 2008
Last modified: September 3, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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