Reviewed, UniProtKB/Swiss-Prot B4UAM8 (SYT_ANASK)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	AnaeK_1896

Organism

Anaeromyxobacter sp. (strain K) [Complete proteome] [HAMAP]

Taxonomic identifier

447217 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 650	650	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_1000098540
Regions
	Region	248 – 548	301	Catalytic HAMAP MF_00184
Sites
	Metal binding	349	1	Zinc; catalytic By similarity
	Metal binding	400	1	Zinc; catalytic By similarity
	Metal binding	525	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4UAM8-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 03000F339CE3B14D	FASTA	650	73,012
	10 20 30 40 50 60 MSDLVKVTLP DGSQKEAPRG TPVIDFVKGQ IGPGLAKAAY FARLDGEPVD LSRAIERDAR 70 80 90 100 110 120 LEIVTTRSPE ALEVARHDAA HVMASVVQKL YPGTQVTIGP AIEDGFYYDF ARETPFTPED 130 140 150 160 170 180 LERIEKATNE AIKADLPFVR SEISMEAALA LFEGMGERYK VEIVKDIAAK GAKTLTLYKH 190 200 210 220 230 240 GDWVDFCLGP HGPSTGRIGV VKLLNVAGAY WRGDAKNAML QRIYGTAFFD KKELDAHLAK 250 260 270 280 290 300 LEEVKKRDHR RLGPQLGLFT FHEFAPGAPF WLPAGTVLYN VLEDAMRRLV LKNGYQEVKT 310 320 330 340 350 360 PLLFNKRLWE TSGHWGKYRE NMFLVVDSES DPALPLEDRC SFSLKPMNCP SHHLIYRMDK 370 380 390 400 410 420 RSYRELPVRY FTTDALHRNE ASGSLGGLTR VRQFEQDDAH IYLREEQVTD EVLRIFELMK 430 440 450 460 470 480 VVYGAFGLGF EATFSTRPEQ RIGDDALWDR AEALLRKSLD ATGLKWTLNA GDGAFYGPKI 490 500 510 520 530 540 DMLVTDSLGR KWQTCTIQLD YAAPERFDLT FVGEDNKEHR PVVIHRAIYG SFERFVAILV 550 560 570 580 590 600 EHYAGAFPAW LAPVQARVVT VSDRFEAWAR EAGEALQARG WRVEVDASSD KLGAKIRNAQ 610 620 630 640 650 LAKIPFTLVV GEKEVEAKGV SPRRHGGEDL KTMPLEAFAE LMTREATAPF

References

[1]

"Complete sequence of Anaeromyxobacter sp. K." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikiva G., Beliaev A. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001131 Genomic DNA. Translation: ACG73124.1.
RefSeq	YP_002134253.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6784690.
GenomeReviews	Gene locus AnaeK_1896 in contig CP001131_GR.
KEGG	ank:AnaeK_1896.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QDEAPGM.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_ANASK

Accession

Primary (citable) accession number: B4UAM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)