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Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Hydrogenobaculum sp. (strain Y04AAS1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

Catalytic activityi

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

Cofactori

a divalent metal cationUniRule annotationNote: Binds 1 divalent metal cation per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei129 – 1291SubstrateUniRule annotation
Metal bindingi158 – 1581Divalent metal cation; shared with dimeric partnerUniRule annotation
Metal bindingi202 – 2021Divalent metal cation; shared with dimeric partnerUniRule annotation
Metal bindingi250 – 2501Divalent metal cation; shared with dimeric partnerUniRule annotation
Binding sitei258 – 2581SubstrateUniRule annotation
Binding sitei267 – 2671SubstrateUniRule annotation
Binding sitei276 – 2761SubstrateUniRule annotation

GO - Molecular functioni

  1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD binding Source: InterPro

GO - Biological processi

  1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
  2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridoxine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, NADP

Enzyme and pathway databases

BioCyciHSP380749:GH30-1008-MONOMER.
UniPathwayiUPA00244; UER00312.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
Gene namesi
Name:pdxAUniRule annotation
Ordered Locus Names:HY04AAS1_0974
OrganismiHydrogenobaculum sp. (strain Y04AAS1)
Taxonomic identifieri380749 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeHydrogenobaculum
ProteomesiUP000001872: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3103104-hydroxythreonine-4-phosphate dehydrogenasePRO_1000146489Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi380749.HY04AAS1_0974.

Structurei

3D structure databases

ProteinModelPortaliB4U950.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PdxA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1995.
HOGENOMiHOG000221591.
KOiK00097.
OMAiTHGDING.
OrthoDBiEOG6GN6ZC.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00536. PdxA.
InterProiIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamiPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00557. pdxA. 1 hit.

Sequencei

Sequence statusi: Complete.

B4U950-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIFGITMGD PAGIGPELIL KLQKDMEDEN AYVIYGEEYI LKHASSVLGQ
60 70 80 90 100
NFYYEKVNSV DDVKDKGIYL ISLSLKGALE PSPSSGKLAI AYLARATADA
110 120 130 140 150
ISKKLNGILT MPINKYFAKA SGFSFNGQTE YLAFADNKKD FAMMMYSDAI
160 170 180 190 200
KVVLATIHIP LKDIANAINV ELIKQKIKLI KDYIPKYFRF IPTIKVLGLN
210 220 230 240 250
PHAGEGGLIG DEEAKIIIPA IRDEDVVGPI PPDTAFIDIK KDDIFLCMYH
260 270 280 290 300
DQGLIPFKML AFDKGSNVTI GLSFLRTSPD HGTAYDIAYK GLARVDSAGY
310
SLELLKRYGY
Length:310
Mass (Da):34,242
Last modified:September 23, 2008 - v1
Checksum:i2C1FDF60B25CB139
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001130 Genomic DNA. Translation: ACG57661.1.
RefSeqiWP_012514017.1. NC_011126.1.
YP_002121639.1. NC_011126.1.

Genome annotation databases

EnsemblBacteriaiACG57661; ACG57661; HY04AAS1_0974.
GeneIDi6743786.
KEGGihya:HY04AAS1_0974.
PATRICi22136638. VBIHydSp64203_0982.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001130 Genomic DNA. Translation: ACG57661.1.
RefSeqiWP_012514017.1. NC_011126.1.
YP_002121639.1. NC_011126.1.

3D structure databases

ProteinModelPortaliB4U950.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi380749.HY04AAS1_0974.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACG57661; ACG57661; HY04AAS1_0974.
GeneIDi6743786.
KEGGihya:HY04AAS1_0974.
PATRICi22136638. VBIHydSp64203_0982.

Phylogenomic databases

eggNOGiCOG1995.
HOGENOMiHOG000221591.
KOiK00097.
OMAiTHGDING.
OrthoDBiEOG6GN6ZC.

Enzyme and pathway databases

UniPathwayiUPA00244; UER00312.
BioCyciHSP380749:GH30-1008-MONOMER.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00536. PdxA.
InterProiIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamiPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00557. pdxA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Y04AAS1.

Entry informationi

Entry nameiPDXA_HYDS0
AccessioniPrimary (citable) accession number: B4U950
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 23, 2008
Last modified: January 7, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is located at the dimer interface.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.