B4U8R9 (NTPA_HYDS0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

HY04AAS1_0843

Organism

Hydrogenobaculum sp. (strain Y04AAS1) [Complete proteome] [HAMAP]

Taxonomic identifier

380749 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Hydrogenobaculum

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_1000145491
Regions
	Region	7 – 12	6	Substrate binding By similarity
	Region	72 – 73	2	Substrate binding By similarity
Sites
	Metal binding	43	1	Magnesium or manganese By similarity
	Metal binding	72	1	Magnesium or manganese By similarity
	Binding site	172	1	Substrate By similarity
	Binding site	190	1	Substrate By similarity
	Binding site	196	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4U8R9 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 697C206A2A94FA6B	FASTA	211	24,059
	10 20 30 40 50 60 MKLMICTSNK KKFEEISSIL ESLKKDENLD LEFVKPPKEI EVEEYANTFL SNAYLKAKAY 70 80 90 100 110 120 YNAFGIPALA DDSGLVVEAF SDNLERPGVY SARFYKDSFG SHVLKEEDFK LSKDELNNLK 130 140 150 160 170 180 VLRLLEKEEN RKAKFVSVVA IVLSNNYGIF GEGELKGHIA KEPFGNFGFG YDPIFIPEGY 190 200 210 NTTLANIENK DKISHRRQAL EAVFFMLKKM L

References

[1]

"Complete and draft genome sequences of six members of the Aquificales." Reysenbach A.-L., Hamamura N., Podar M., Griffiths E., Ferreira S., Hochstein R., Heidelberg J., Johnson J., Mead D., Pohorille A., Sarmiento M., Schweighofer K., Seshadri R., Voytek M.A. J. Bacteriol. 191:1992-1993(2009) [PubMed: 19136599] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Y04AAS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001130 Genomic DNA. Translation: ACG57530.1.
RefSeq	YP_002121508.1. NC_011126.1.
3D structure databases
ProteinModelPortal	B4U8R9.
ModBase	Search...
Protein-protein interaction databases
STRING	B4U8R9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6743654.
GenomeReviews	Gene locus HY04AAS1_0843 in contig CP001130_GR.
KEGG	hya:HY04AAS1_0843.
PATRIC	22136374. VBIHydSp64203_0850.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG697237.
OMA	GVYTADW.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_HYDS0

Accession

Primary (citable) accession number: B4U8R9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program