B4U8M6 (GSA_HYDS0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase
Short name=GSA
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name=GSA-AT

Gene names

Name:	hemL
Ordered Locus Names:	HY04AAS1_0800

Organism

Hydrogenobaculum sp. (strain Y04AAS1) [Complete proteome] [HAMAP]

Taxonomic identifier

380749 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Aquificae › Aquificae › Aquificales › Aquificaceae › Hydrogenobaculum

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate. HAMAP-Rule MF_00375
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. HAMAP-Rule MF_00375
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00375.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Glutamate-1-semialdehyde 2,1-aminomutase HAMAP-Rule MF_00375		PRO_1000121897
Amino acid modifications
	Modified residue	264	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4U8M6 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: CF75F55D729E3036	FASTA	425	46,312
	10 20 30 40 50 60 MTNKEAFELA KKYMPGGVSS PVRAFKSVGA EPIVVNRGFG AFVEDIEGKK YIDYMLSFGP 70 80 90 100 110 120 LILGHRSEIV VSKIMEALDK GNSFGITNMY EIELSELIIK ASKVIDKVRF VSSGTEAVMS 130 140 150 160 170 180 AIRLARGITN KPYIIKFDGC YHGFSDSVLV GAGSGVATLG IPGTPGIPKE FAALTIVLDY 190 200 210 220 230 240 NDENALEEAF IKYKNQIAAV LVEPVAGNMG VVLPKESWLK KLRDITKEND ALLIFDEVIT 250 260 270 280 290 300 GFRLALGGAA EYFGIEPDIV CYGKIIGGGM PIGAYGAKNH IMERVAPEGP IYQAGTLSGN 310 320 330 340 350 360 PISVASGLAI LKTLIKDIAI YDRLSELRAY LTYTLSKMLS EKGIPHRINE IASMFTIFFT 370 380 390 400 410 420 DKDVIDFKSA KTSDTALFGK FFRNALKEGV LMPPSQFEAW FLSTAHTKDV VDTTLEKLKK AIDLL

References

[1]

"Complete and draft genome sequences of six members of the Aquificales." Reysenbach A.-L., Hamamura N., Podar M., Griffiths E., Ferreira S., Hochstein R., Heidelberg J., Johnson J., Mead D., Pohorille A., Sarmiento M., Schweighofer K., Seshadri R., Voytek M.A. J. Bacteriol. 191:1992-1993(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Y04AAS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001130 Genomic DNA. Translation: ACG57487.1.
RefSeq	YP_002121465.1. NC_011126.1.
3D structure databases
ProteinModelPortal	B4U8M6.
SMR	B4U8M6. Positions 3-422.
ModBase	Search...
Protein-protein interaction databases
STRING	380749.HY04AAS1_0800.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACG57487; ACG57487; HY04AAS1_0800.
GeneID	6743606.
KEGG	hya:HY04AAS1_0800.
PATRIC	22136276. VBIHydSp64203_0806.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0001.
HOGENOM	HOG000020210.
KO	K01845.
OMA	AYLTDMD.
Enzyme and pathway databases
BioCyc	HSP380749:GH30-828-MONOMER.
UniPathway	UPA00251; UER00317.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
HAMAP	MF_00375. HemL_aminotrans_3.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. hemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_HYDS0

Accession

Primary (citable) accession number: B4U8M6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program