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Protein

Glutamate-1-semialdehyde 2,1-aminomutase

Gene

hemL

Organism
Hydrogenobaculum sp. (strain Y04AAS1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-4-amino-5-oxopentanoate = 5-aminolevulinate.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathway: protoporphyrin-IX biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamyl-tRNA reductase (hemA)
  2. Glutamate-1-semialdehyde 2,1-aminomutase (hemL)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Porphyrin biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciHSP380749:GH30-828-MONOMER.
UniPathwayiUPA00251; UER00317.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutaseUniRule annotation (EC:5.4.3.8UniRule annotation)
Short name:
GSAUniRule annotation
Alternative name(s):
Glutamate-1-semialdehyde aminotransferaseUniRule annotation
Short name:
GSA-ATUniRule annotation
Gene namesi
Name:hemLUniRule annotation
Ordered Locus Names:HY04AAS1_0800
OrganismiHydrogenobaculum sp. (strain Y04AAS1)
Taxonomic identifieri380749 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeHydrogenobaculum
ProteomesiUP000001872 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 425425Glutamate-1-semialdehyde 2,1-aminomutasePRO_1000121897Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei264 – 2641N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi380749.HY04AAS1_0800.

Structurei

3D structure databases

ProteinModelPortaliB4U8M6.
SMRiB4U8M6. Positions 3-422.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0001.
HOGENOMiHOG000020210.
KOiK01845.
OMAiCGHAHPE.
OrthoDBiEOG6QVRHN.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_00375. HemL_aminotrans_3.
InterProiIPR004639. 4pyrrol_synth_GluAld_NH2Trfase.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00713. hemL. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4U8M6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNKEAFELA KKYMPGGVSS PVRAFKSVGA EPIVVNRGFG AFVEDIEGKK
60 70 80 90 100
YIDYMLSFGP LILGHRSEIV VSKIMEALDK GNSFGITNMY EIELSELIIK
110 120 130 140 150
ASKVIDKVRF VSSGTEAVMS AIRLARGITN KPYIIKFDGC YHGFSDSVLV
160 170 180 190 200
GAGSGVATLG IPGTPGIPKE FAALTIVLDY NDENALEEAF IKYKNQIAAV
210 220 230 240 250
LVEPVAGNMG VVLPKESWLK KLRDITKEND ALLIFDEVIT GFRLALGGAA
260 270 280 290 300
EYFGIEPDIV CYGKIIGGGM PIGAYGAKNH IMERVAPEGP IYQAGTLSGN
310 320 330 340 350
PISVASGLAI LKTLIKDIAI YDRLSELRAY LTYTLSKMLS EKGIPHRINE
360 370 380 390 400
IASMFTIFFT DKDVIDFKSA KTSDTALFGK FFRNALKEGV LMPPSQFEAW
410 420
FLSTAHTKDV VDTTLEKLKK AIDLL
Length:425
Mass (Da):46,312
Last modified:September 23, 2008 - v1
Checksum:iCF75F55D729E3036
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001130 Genomic DNA. Translation: ACG57487.1.
RefSeqiWP_012513843.1. NC_011126.1.
YP_002121465.1. NC_011126.1.

Genome annotation databases

EnsemblBacteriaiACG57487; ACG57487; HY04AAS1_0800.
KEGGihya:HY04AAS1_0800.
PATRICi22136276. VBIHydSp64203_0806.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001130 Genomic DNA. Translation: ACG57487.1.
RefSeqiWP_012513843.1. NC_011126.1.
YP_002121465.1. NC_011126.1.

3D structure databases

ProteinModelPortaliB4U8M6.
SMRiB4U8M6. Positions 3-422.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi380749.HY04AAS1_0800.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACG57487; ACG57487; HY04AAS1_0800.
KEGGihya:HY04AAS1_0800.
PATRICi22136276. VBIHydSp64203_0806.

Phylogenomic databases

eggNOGiCOG0001.
HOGENOMiHOG000020210.
KOiK01845.
OMAiCGHAHPE.
OrthoDBiEOG6QVRHN.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00317.
BioCyciHSP380749:GH30-828-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_00375. HemL_aminotrans_3.
InterProiIPR004639. 4pyrrol_synth_GluAld_NH2Trfase.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00713. hemL. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Y04AAS1.

Entry informationi

Entry nameiGSA_HYDS0
AccessioniPrimary (citable) accession number: B4U8M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: April 1, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.