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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Hydrogenobaculum sp. (strain Y04AAS1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei583Aminoacyl-adenylateUniRule annotation1
Binding sitei627ATPUniRule annotation1
Metal bindingi915ZincUniRule annotation1
Metal bindingi918ZincUniRule annotation1
Metal bindingi930ZincUniRule annotation1
Metal bindingi933ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHSP380749:G1GCJ-786-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:HY04AAS1_0775
OrganismiHydrogenobaculum sp. (strain Y04AAS1)
Taxonomic identifieri380749 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeHydrogenobaculum
Proteomesi
  • UP000001872 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002162371 – 937Isoleucine--tRNA ligaseAdd BLAST937

Proteomic databases

PRIDEiB4U8K1

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi380749.HY04AAS1_0775

Structurei

3D structure databases

ProteinModelPortaliB4U8K1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi55 – 65"HIGH" regionAdd BLAST11
Motifi624 – 628"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 3 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B4U8K1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKDTINLP KTDFPMKANL SEKELEILKK WEGLYEKLRA QRKGKQLFVL
60 70 80 90 100
HDGPPYANGN IHIGHALNKI LKDIICKVAL IEGFDVDYKP GWDCHGLPIE
110 120 130 140 150
QQVEKELKAK KINKESMPKD EFRRLCREYA SKFVSIQKEE FLRLGVLGDW
160 170 180 190 200
ENPYLTMDPK YEAQEVREIG RCLQNGVLYK GNKPVYWCIY DKTAEAEAEV
210 220 230 240 250
EYKEKKDISI YVRFELLKQS ENVLRQKLDL PDKPISIVIW TTTPWTLPAN
260 270 280 290 300
LGVMSSEDIE YVVVEEDNYL LLLEKQSQYV KNKEFIASLK GKELTGLEYQ
310 320 330 340 350
HPFIERTGKI YPSEFVEAGT GTGFVHMAPG HGMEDYIVGL RYGLEPFSPV
360 370 380 390 400
DESGRFTEEA PEFIRGLNVF EANTVIIEHL KQTGFLLKEE EILHSYPHCW
410 420 430 440 450
RCKNPVIYRA TPQWFIGVDR AITSHFASCE DEKIDTSDVK KLQNKSIREK
460 470 480 490 500
AIEETYNVKW VPKTGQNRML SMLQNRPDWC ISRQRFWGVP ITIFYCKRCG
510 520 530 540 550
EPLLESHIFE HVAKIFESSP YGADEWFKKD EKELLPPDTV CKKCGSKDFV
560 570 580 590 600
KETDILDVWF DSGSSHASVL RPRGIEKADV YLEGSDQHRG WFQASLLESI
610 620 630 640 650
ASYGEAPFKS VVTHGFTVDE KGHKMSKSQG NVISPLEIIK EYGADILRLW
660 670 680 690 700
VISEDYTEDI KLGKSILKRL AEDYKKIRNT LRYCLGNLYD FKYEFSIAPD
710 720 730 740 750
KLHHFDRYMY AYASKVFEEL FSFYKNYQYH RFYHRLMEFV SIDLSALYFD
760 770 780 790 800
VLKDRLYMYK SGSFERLSAQ TVLYFLLKNL TILLSPVLSF TAEETYSYMK
810 820 830 840 850
SIENHLPESI FMNEYKASDF TDEELLKDYQ KLLLLRKDVL KAIEIERKND
860 870 880 890 900
IIKHPYEARV VIKPNEEFYK LLEKYKDYLH SFFTVSQVEI AENDGEEGEY
910 920 930
TKLRIKVEKA KGEKCPRCWL YVETMINGVC PRCSQNI
Length:937
Mass (Da):109,507
Last modified:September 23, 2008 - v1
Checksum:i833B423E2424492E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001130 Genomic DNA Translation: ACG57462.1
RefSeqiWP_012513818.1, NC_011126.1

Genome annotation databases

EnsemblBacteriaiACG57462; ACG57462; HY04AAS1_0775
KEGGihya:HY04AAS1_0775

Similar proteinsi

Entry informationi

Entry nameiSYI_HYDS0
AccessioniPrimary (citable) accession number: B4U8K1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 23, 2008
Last modified: March 28, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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