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B4U8K1

- SYI_HYDS0

UniProt

B4U8K1 - SYI_HYDS0

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Hydrogenobaculum sp. (strain Y04AAS1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei583 – 5831Aminoacyl-adenylateUniRule annotation
Binding sitei627 – 6271ATPUniRule annotation
Metal bindingi915 – 9151ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation
Metal bindingi930 – 9301ZincUniRule annotation
Metal bindingi933 – 9331ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHSP380749:GH30-802-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:HY04AAS1_0775
OrganismiHydrogenobaculum sp. (strain Y04AAS1)
Taxonomic identifieri380749 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeHydrogenobaculum
ProteomesiUP000001872: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 937937Isoleucine--tRNA ligasePRO_1000216237Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi380749.HY04AAS1_0775.

Structurei

3D structure databases

ProteinModelPortaliB4U8K1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi55 – 6511"HIGH" regionAdd
BLAST
Motifi624 – 6285"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4U8K1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKDTINLP KTDFPMKANL SEKELEILKK WEGLYEKLRA QRKGKQLFVL
60 70 80 90 100
HDGPPYANGN IHIGHALNKI LKDIICKVAL IEGFDVDYKP GWDCHGLPIE
110 120 130 140 150
QQVEKELKAK KINKESMPKD EFRRLCREYA SKFVSIQKEE FLRLGVLGDW
160 170 180 190 200
ENPYLTMDPK YEAQEVREIG RCLQNGVLYK GNKPVYWCIY DKTAEAEAEV
210 220 230 240 250
EYKEKKDISI YVRFELLKQS ENVLRQKLDL PDKPISIVIW TTTPWTLPAN
260 270 280 290 300
LGVMSSEDIE YVVVEEDNYL LLLEKQSQYV KNKEFIASLK GKELTGLEYQ
310 320 330 340 350
HPFIERTGKI YPSEFVEAGT GTGFVHMAPG HGMEDYIVGL RYGLEPFSPV
360 370 380 390 400
DESGRFTEEA PEFIRGLNVF EANTVIIEHL KQTGFLLKEE EILHSYPHCW
410 420 430 440 450
RCKNPVIYRA TPQWFIGVDR AITSHFASCE DEKIDTSDVK KLQNKSIREK
460 470 480 490 500
AIEETYNVKW VPKTGQNRML SMLQNRPDWC ISRQRFWGVP ITIFYCKRCG
510 520 530 540 550
EPLLESHIFE HVAKIFESSP YGADEWFKKD EKELLPPDTV CKKCGSKDFV
560 570 580 590 600
KETDILDVWF DSGSSHASVL RPRGIEKADV YLEGSDQHRG WFQASLLESI
610 620 630 640 650
ASYGEAPFKS VVTHGFTVDE KGHKMSKSQG NVISPLEIIK EYGADILRLW
660 670 680 690 700
VISEDYTEDI KLGKSILKRL AEDYKKIRNT LRYCLGNLYD FKYEFSIAPD
710 720 730 740 750
KLHHFDRYMY AYASKVFEEL FSFYKNYQYH RFYHRLMEFV SIDLSALYFD
760 770 780 790 800
VLKDRLYMYK SGSFERLSAQ TVLYFLLKNL TILLSPVLSF TAEETYSYMK
810 820 830 840 850
SIENHLPESI FMNEYKASDF TDEELLKDYQ KLLLLRKDVL KAIEIERKND
860 870 880 890 900
IIKHPYEARV VIKPNEEFYK LLEKYKDYLH SFFTVSQVEI AENDGEEGEY
910 920 930
TKLRIKVEKA KGEKCPRCWL YVETMINGVC PRCSQNI
Length:937
Mass (Da):109,507
Last modified:September 23, 2008 - v1
Checksum:i833B423E2424492E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001130 Genomic DNA. Translation: ACG57462.1.
RefSeqiWP_012513818.1. NC_011126.1.
YP_002121440.1. NC_011126.1.

Genome annotation databases

EnsemblBacteriaiACG57462; ACG57462; HY04AAS1_0775.
GeneIDi6743580.
KEGGihya:HY04AAS1_0775.
PATRICi22136222. VBIHydSp64203_0780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001130 Genomic DNA. Translation: ACG57462.1 .
RefSeqi WP_012513818.1. NC_011126.1.
YP_002121440.1. NC_011126.1.

3D structure databases

ProteinModelPortali B4U8K1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 380749.HY04AAS1_0775.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACG57462 ; ACG57462 ; HY04AAS1_0775 .
GeneIDi 6743580.
KEGGi hya:HY04AAS1_0775.
PATRICi 22136222. VBIHydSp64203_0780.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HSP380749:GH30-802-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Y04AAS1.

Entry informationi

Entry nameiSYI_HYDS0
AccessioniPrimary (citable) accession number: B4U8K1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 23, 2008
Last modified: October 29, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3