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B4U896

- SYE_HYDS0

UniProt

B4U896 - SYE_HYDS0

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Hydrogenobaculum sp. (strain Y04AAS1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi96 – 961ZincUniRule annotation
    Metal bindingi98 – 981ZincUniRule annotation
    Metal bindingi123 – 1231ZincUniRule annotation
    Metal bindingi125 – 1251ZincUniRule annotation
    Binding sitei243 – 2431ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciHSP380749:GH30-692-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:HY04AAS1_0670
    OrganismiHydrogenobaculum sp. (strain Y04AAS1)
    Taxonomic identifieri380749 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeHydrogenobaculum
    ProteomesiUP000001872: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 464464Glutamate--tRNA ligasePRO_0000367690Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi380749.HY04AAS1_0670.

    Structurei

    3D structure databases

    ProteinModelPortaliB4U896.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi8 – 1811"HIGH" regionAdd
    BLAST
    Motifi240 – 2445"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252720.
    KOiK01885.
    OMAiAMGWEVP.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    1.10.8.70. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B4U896-1 [UniParc]FASTAAdd to Basket

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    MVRTRFAPSP TGYMHLGNAR TGIFSYLFAR HHNGTFVLRI EDTDRERSTK    50
    EFEDSIIEDL KWLGIEWDEF YRQSERFDIY KEYAKKLIDS GHAYYCFCKE 100
    EDIEKQREEA YAQGKAYRYP GTCRHLSKED VEDRLKSGES YVIRFKVPDG 150
    QIVSFEDMIR GNISINVDDF GDFVIVRSDG SSVYNFVAVI DDALMKITHV 200
    IRGEDHISNT PKQILIYEAL GFKPPEFAHL PVILGEDRTK LSKRHGGVSV 250
    RFYKENGYCP EALFNYLCLL GWSSEKVGKE VISKEEAVKY FDIKDINLSP 300
    AVFSHDKLYW LNGVYIREIL PEDRLLEDLL SFLEKAYGTV DIEYLKKIVK 350
    ATRKEYNTYL EAVEKLRPFF KEKELDEVAK EELSKIDRKV FELLKQEIES 400
    LEEITPENLK GVVKNIQKST SLKPKDVWHA LRIALTGSLE GIAIDVIASI 450
    LPKEEVLKRL SRYT 464
    Length:464
    Mass (Da):53,877
    Last modified:September 23, 2008 - v1
    Checksum:iE320C4BEF14DE1BE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001130 Genomic DNA. Translation: ACG57357.1.
    RefSeqiWP_012513713.1. NC_011126.1.
    YP_002121335.1. NC_011126.1.

    Genome annotation databases

    EnsemblBacteriaiACG57357; ACG57357; HY04AAS1_0670.
    GeneIDi6743470.
    KEGGihya:HY04AAS1_0670.
    PATRICi22135998. VBIHydSp64203_0673.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001130 Genomic DNA. Translation: ACG57357.1 .
    RefSeqi WP_012513713.1. NC_011126.1.
    YP_002121335.1. NC_011126.1.

    3D structure databases

    ProteinModelPortali B4U896.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 380749.HY04AAS1_0670.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACG57357 ; ACG57357 ; HY04AAS1_0670 .
    GeneIDi 6743470.
    KEGGi hya:HY04AAS1_0670.
    PATRICi 22135998. VBIHydSp64203_0673.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252720.
    KOi K01885.
    OMAi AMGWEVP.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci HSP380749:GH30-692-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    1.10.8.70. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Y04AAS1.

    Entry informationi

    Entry nameiSYE_HYDS0
    AccessioniPrimary (citable) accession number: B4U896
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3