B4U838 (B4U838_HYDS0) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685
Ordered Locus Names:	HY04AAS1_0612

Organism

Hydrogenobaculum sp. (strain Y04AAS1) [Complete proteome] [HAMAP]

Taxonomic identifier

380749 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Hydrogenobaculum

Protein attributes

Sequence length	631 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	312	1	Nucleophile By similarity HAMAP MF_00685
	Active site	365	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	B4U838 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 330730F751E42C83	FASTA	631	74,115
	10 20 30 40 50 60 MAYYKYNYDI SLLTDEDLYL FNEGTHYHLY EKLGAHVLEG GVYFAVWAPN AKEVYVEGSF 70 80 90 100 110 120 NFWNKKEYPL RPKGSSGIWE IFIEGLKSYE LYKYYIVSNF GYEVEKRDPF AFYTEVPPKS 130 140 150 160 170 180 AGIIYDISGY QWKDDLWLSK RRQSNHYKEP ISIYEVHLGS WRRTLENEFL SYKDIAHQLV 190 200 210 220 230 240 EYVKDMGFTH VEFLPIMEHP FYGSWGYQTL SYFAPTSRHG EPKDFMYLVE LLHQNDIGVI 250 260 270 280 290 300 LDWVPSHFAT DDYGLSFFDG THLYEHMDKR KGYHPDWGSY IFNYGRHEVR AFLISSANFW 310 320 330 340 350 360 FNKYHVDGIR VDAVASMLYL DYSRKEGEWE PNIYGGKENL EAIDCLRKLN EAVYIFNPGI 370 380 390 400 410 420 FTVAEESTAW PMVTKPTYIG GLGFGFKWDM GWMHDTLNYF SKDPVHRKYH QQDITFRMLY 430 440 450 460 470 480 AFNENFILPL SHDEVVHGKG SLIGKMPGDY WQKFANLRLL LSYMYGSPGK KLLFMGSEFG 490 500 510 520 530 540 QFDEWNHDKS LDWHLLEFDS HKGIQNLVKD LNRLYHQEAA LYELDHDPEG FEWIDFQDVE 550 560 570 580 590 600 QSIFSFLRKA KNGDFVICIF NATPVPRHDY KIGVPKPGFY KEILNSDSMY YWGSNVGNAG 610 620 630 LIEAHAEPWH KFNFSISLTL PPLGALFLKL A

References

[1]

"Complete and draft genome sequences of six members of the Aquificales." Reysenbach A.-L., Hamamura N., Podar M., Griffiths E., Ferreira S., Hochstein R., Heidelberg J., Johnson J., Mead D., Pohorille A., Sarmiento M., Schweighofer K., Seshadri R., Voytek M.A. J. Bacteriol. 191:1992-1993(2009) [PubMed: 19136599] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001130 Genomic DNA. Translation: ACG57299.1.
RefSeq	YP_002121277.1. NC_011126.1.
3D structure databases
ProteinModelPortal	B4U838.
ModBase	Search...
Protein-protein interaction databases
STRING	B4U838.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6743409.
GenomeReviews	Gene locus HY04AAS1_0612 in contig CP001130_GR.
KEGG	hya:HY04AAS1_0612.
PATRIC	22135878. VBIHydSp64203_0616.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	RVYHQNG.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B4U838_HYDS0

Accession

Primary (citable) accession number: B4U838

Entry history

Integrated into UniProtKB/TrEMBL:	September 23, 2008
Last sequence update:	September 23, 2008
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)