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B4U7D7 (DAPA_HYDS0) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:HY04AAS1_0358
OrganismHydrogenobaculum sp. (strain Y04AAS1) [Complete proteome] [HAMAP]
Taxonomic identifier380749 [NCBI]
Taxonomic lineageBacteria › Aquificae › Aquificales › Aquificaceae › Hydrogenobaculum

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subunit structure

Homotetramer By similarity. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 290290Dihydrodipicolinate synthase HAMAP MF_00418
PRO_1000124043

Regions

Region47 – 482Pyruvate binding By similarity

Sites

Active site1611Schiff-base intermediate with substrate By similarity
Binding site1051Pyruvate By similarity
Site1321Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
B4U7D7 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 756642A8F3E35D29

FASTA29032,510
        10         20         30         40         50         60 
MLTGSITAIV TPFKNGEVDY GAFERLIEFQ IQNGTDGILV CGTSGESPTL SYEEHEAVIE 

        70         80         90        100        110        120 
FAVKSAKKRI HIMAGTGANS TEEALRFTTF AKAVGADSAL LVVPYYNKPT QEGLYRHFSK 

       130        140        150        160        170        180 
IAKEVDIDIY IYNIPSRTGI EISVDTLERL AKDHKNIKGS KESTPNMDRI SEILKRIPNF 

       190        200        210        220        230        240 
TVFSGDDSLT LPMMSLGAKG VVSVISNVMP KEIKEFTSYA LKGDFEKARD MHYYLLEIFK 

       250        260        270        280        290 
IMFIETNPIP VKTALSLMGM VKKEFRLPLC EMLPQNEEKL KEVLKKYNLI

« Hide

References

[1]"Complete and draft genome sequences of six members of the Aquificales."
Reysenbach A.-L., Hamamura N., Podar M., Griffiths E., Ferreira S., Hochstein R., Heidelberg J., Johnson J., Mead D., Pohorille A., Sarmiento M., Schweighofer K., Seshadri R., Voytek M.A.
J. Bacteriol. 191:1992-1993(2009) [PubMed: 19136599] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Y04AAS1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001130 Genomic DNA. Translation: ACG57048.1.
RefSeqYP_002121026.1. NC_011126.1.

3D structure databases

ProteinModelPortalB4U7D7.
SMRB4U7D7. Positions 1-289.
ModBaseSearch...

Protein-protein interaction databases

STRINGB4U7D7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6743152.
GenomeReviewsGene locus HY04AAS1_0358 in contig CP001130_GR.
KEGGhya:HY04AAS1_0358.
PATRIC22135356. VBIHydSp64203_0357.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG358848.
OMAFMLCGGH.
ProtClustDBPRK03170.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. False negative.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_HYDS0
AccessionPrimary (citable) accession number: B4U7D7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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