B4U6Z8 (ILVD_HYDS0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	HY04AAS1_0217

Organism

Hydrogenobaculum sp. (strain Y04AAS1) [Complete proteome] [HAMAP]

Taxonomic identifier

380749 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Hydrogenobaculum

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000089390
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	191	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	B4U6Z8 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: E84678393AB64EF3	FASTA	554	59,771
	10 20 30 40 50 60 MRSDIIQKGY DKAPHRSLLR ACGFKDEDFG KPIIGVANSY IDIVPGHVHL REFVEPIKEE 70 80 90 100 110 120 IRKAGAIPVE FNTIGVDDGI AMGHYGMHYS LPSRELIADA IETVVEAHQL DGLICIPNCD 130 140 150 160 170 180 KIVPGMLMGA LRVNVPTVFI SGGPMAAGKI GDKKVDLISV FEGVGKLNKG EITEKDLLVI 190 200 210 220 230 240 EQNACPSCGS CSGLFTANSM NCLTEVLGLG LPGNGTTLAI DPRREMLARQ AARQVVELVK 250 260 270 280 290 300 IDLKPRDIVT KASFDNAFRV DIAMGGSSNT VLHLLAIARE AGIEYKMEDI DKISRETPTL 310 320 330 340 350 360 CKISPSSDYH MDDLDRAGGI SAIMKELLRN GLFDGKQRTV TTKTIEEIVK DVEIMDENVI 370 380 390 400 410 420 RRIDNAYSKD GGLAILFGNL APKGSVVKTA GVAKEMLQFK GKAICFDSEE EAIEGIRGGK 430 440 450 460 470 480 VKPGHVVVIR YEGPKGGPGM REMLSPTSTI MGMGLGSSVA LITDGRFSGG TRGACIGHIS 490 500 510 520 530 540 PEAAAGGPIG IVQDGDEILI DIPNRKLELL ISQEEFDARL KAFKPKEKLI KSSWLKRYRK 550 FVKDASEGAI LSAD

References

[1]

"Complete and draft genome sequences of six members of the Aquificales." Reysenbach A.-L., Hamamura N., Podar M., Griffiths E., Ferreira S., Hochstein R., Heidelberg J., Johnson J., Mead D., Pohorille A., Sarmiento M., Schweighofer K., Seshadri R., Voytek M.A. J. Bacteriol. 191:1992-1993(2009) [PubMed: 19136599] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Y04AAS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001130 Genomic DNA. Translation: ACG56909.1.
RefSeq	YP_002120887.1. NC_011126.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B4U6Z8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6743006.
GenomeReviews	Gene locus HY04AAS1_0217 in contig CP001130_GR.
KEGG	hya:HY04AAS1_0217.
PATRIC	22135064. VBIHydSp64203_0216.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_HYDS0

Accession

Primary (citable) accession number: B4U6Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program